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Regulation of skeletal muscle PPAR delta mRNA expression in twins

Nilsson, Emma; Poulsen, Pernille; Sjögren, Marketa LU ; Ling, Charlotte LU ; Ridderstråle, Martin LU ; Groop, Leif LU and Vaag, Allan (2007) In Journal of Physiology 584(3). p.1011-1017
Abstract
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors regulating the expression of genes involved in lipid and glucose metabolism in a complex and to some extent unknown manner. Our aim was to study the impact of different factors on PPAR delta mRNA expression in human skeletal muscle on one side, and the impact of PPAR delta mRNA expression on these factors, including glucose and lipid metabolism, aerobic capacity, fibre type composition and lipid profile, on the other side. PPAR delta mRNA levels were quantified by real-time PCR in muscle biopsies from 176 young and elderly monozygotic and dizygotic twins. Young twins had significantly increased PPAR delta mRNA levels compared with elderly twins. A 2 h... (More)
Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors regulating the expression of genes involved in lipid and glucose metabolism in a complex and to some extent unknown manner. Our aim was to study the impact of different factors on PPAR delta mRNA expression in human skeletal muscle on one side, and the impact of PPAR delta mRNA expression on these factors, including glucose and lipid metabolism, aerobic capacity, fibre type composition and lipid profile, on the other side. PPAR delta mRNA levels were quantified by real-time PCR in muscle biopsies from 176 young and elderly monozygotic and dizygotic twins. Young twins had significantly increased PPAR delta mRNA levels compared with elderly twins. A 2 h hyperinsulinaemic euglycaemic clamp had no significant effect on PPAR delta mRNA levels. Biometric models were calculated for basal PPAR delta mRNA expression to estimate the degree of genetic versus environmental influence. In both young and elderly twins there was a substantial genetic component influencing basal PPAR delta mRNA levels. In a regression model, the muscle PPAR delta mRNA expression was correlated to birth weight, central adiposity and age. The level of PPAR delta mRNA was also positively correlated with markers for oxidative muscle fibres. However, in this apparently healthy study population, we found no correlations between PPAR delta mRNA expression and aerobic capacity, lipid profile or glucose and lipid metabolism. In conclusion, we provide evidence that mRNA expression of PPAR delta in human skeletal muscle is under genetic control but also influenced by factors such as age, birth weight and central adiposity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Physiology
volume
584
issue
3
pages
1011 - 1017
publisher
The Physiological Society
external identifiers
  • wos:000251132100024
  • scopus:35648949329
ISSN
1469-7793
DOI
10.1113/jphysiol.2007.140673
language
English
LU publication?
yes
id
1207e3fb-0771-4de5-97c9-ad4692174556 (old id 969005)
alternative location
http://jp.physoc.org/cgi/content/abstract/584/3/1011
date added to LUP
2008-01-30 07:16:34
date last changed
2017-04-16 04:14:50
@article{1207e3fb-0771-4de5-97c9-ad4692174556,
  abstract     = {Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors regulating the expression of genes involved in lipid and glucose metabolism in a complex and to some extent unknown manner. Our aim was to study the impact of different factors on PPAR delta mRNA expression in human skeletal muscle on one side, and the impact of PPAR delta mRNA expression on these factors, including glucose and lipid metabolism, aerobic capacity, fibre type composition and lipid profile, on the other side. PPAR delta mRNA levels were quantified by real-time PCR in muscle biopsies from 176 young and elderly monozygotic and dizygotic twins. Young twins had significantly increased PPAR delta mRNA levels compared with elderly twins. A 2 h hyperinsulinaemic euglycaemic clamp had no significant effect on PPAR delta mRNA levels. Biometric models were calculated for basal PPAR delta mRNA expression to estimate the degree of genetic versus environmental influence. In both young and elderly twins there was a substantial genetic component influencing basal PPAR delta mRNA levels. In a regression model, the muscle PPAR delta mRNA expression was correlated to birth weight, central adiposity and age. The level of PPAR delta mRNA was also positively correlated with markers for oxidative muscle fibres. However, in this apparently healthy study population, we found no correlations between PPAR delta mRNA expression and aerobic capacity, lipid profile or glucose and lipid metabolism. In conclusion, we provide evidence that mRNA expression of PPAR delta in human skeletal muscle is under genetic control but also influenced by factors such as age, birth weight and central adiposity.},
  author       = {Nilsson, Emma and Poulsen, Pernille and Sjögren, Marketa and Ling, Charlotte and Ridderstråle, Martin and Groop, Leif and Vaag, Allan},
  issn         = {1469-7793},
  language     = {eng},
  number       = {3},
  pages        = {1011--1017},
  publisher    = {The Physiological Society},
  series       = {Journal of Physiology},
  title        = {Regulation of skeletal muscle PPAR delta mRNA expression in twins},
  url          = {http://dx.doi.org/10.1113/jphysiol.2007.140673},
  volume       = {584},
  year         = {2007},
}