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Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI-TOF MS

Ekström, Simon LU ; Wallman, Lars LU ; Helldin, Goran; Nilsson, Johan LU ; Marko-Varga, György LU and Laurell, Thomas LU (2007) In Journal of Mass Spectrometry 42(11). p.1445-1452
Abstract
d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide... (More)
d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide capture is also demonstrated. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
polymeric MALDI, microfluidics, SPE, MALDI MS, solid-phase extraction, ISET, target
in
Journal of Mass Spectrometry
volume
42
issue
11
pages
1445 - 1452
publisher
John Wiley & Sons
external identifiers
  • wos:000250987600007
  • scopus:36148999491
ISSN
1076-5174
DOI
10.1002/jms.1278
project
CREATE Health
language
English
LU publication?
yes
id
c67db9b5-2df2-445a-8301-1dd2786008c8 (old id 969020)
date added to LUP
2008-01-29 14:53:14
date last changed
2017-09-10 04:29:18
@article{c67db9b5-2df2-445a-8301-1dd2786008c8,
  abstract     = {d A polymer microfabricated proteomic sample preparation and MALDI MS sample presentation device, the integrated selective enrichment target (ISET), comprising an array of perforated nanovials is reported. Each perforated nanovial can be filled with selective extraction media (microbeads) for purification and concentration of protein/peptides prior to matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS). The main areas covered are the influence of the molding-process-induced surface roughness and how to address the lack of inherent conductivity in the polyetheretherketone (PEEK) material for optimal MALDI MS readout. Application of the disposable polymeric ISET devices for solid-phase extraction and phosphopeptide capture is also demonstrated.},
  author       = {Ekström, Simon and Wallman, Lars and Helldin, Goran and Nilsson, Johan and Marko-Varga, György and Laurell, Thomas},
  issn         = {1076-5174},
  keyword      = {polymeric MALDI,microfluidics,SPE,MALDI MS,solid-phase extraction,ISET,target},
  language     = {eng},
  number       = {11},
  pages        = {1445--1452},
  publisher    = {John Wiley & Sons},
  series       = {Journal of Mass Spectrometry},
  title        = {Polymeric integrated selective enrichment target (ISET) for solid-phase-based sample preparation in MALDI-TOF MS},
  url          = {http://dx.doi.org/10.1002/jms.1278},
  volume       = {42},
  year         = {2007},
}