Advanced

Elucidation of the phenotypic, functional, and molecular topography of a myeloerythroid progenitor cell hierarchy

Pronk, Cornelis J. H.; Rossi, Derrick J.; Månsson, Robert LU ; Attema, Joanne LU ; Norddahl, Gudmundur LU ; Chan, Charles Kwok Fai; Sigvardsson, Mikael LU ; Weissman, Irving L. and Bryder, David LU (2007) In Cell Stem Cell 1(4). p.428-442
Abstract
The major myeloid blood cell lineages are generated from hematopoietic stem cells by differentiation through a series of increasingly committed progenitor cells. Precise characterization of intermediate progenitors is important for understanding fundamental differentiation processes and a variety of disease states, including leukemia. Here, we evaluated the functional in vitro and in vivo potentials of a range of prospectively isolated myeloid precursors with differential expression of CD150, Endoglin, and CD41. Our studies revealed a hierarchy of myeloerythroid progenitors with distinct lineage potentials. The global gene expression signatures of these subsets were consistent with their functional capacities, and hierarchical clustering... (More)
The major myeloid blood cell lineages are generated from hematopoietic stem cells by differentiation through a series of increasingly committed progenitor cells. Precise characterization of intermediate progenitors is important for understanding fundamental differentiation processes and a variety of disease states, including leukemia. Here, we evaluated the functional in vitro and in vivo potentials of a range of prospectively isolated myeloid precursors with differential expression of CD150, Endoglin, and CD41. Our studies revealed a hierarchy of myeloerythroid progenitors with distinct lineage potentials. The global gene expression signatures of these subsets were consistent with their functional capacities, and hierarchical clustering analysis suggested likely lineage relationships. These studies provide valuable tools for understanding myeloid lineage commitment, including isolation of an early erythroid-restricted precursor, and add to existing models of hematopoietic differentiation by suggesting that progenitors of the innate and adaptive immune system can separate late, following the divergence of megakaryocytic/erythroid potential. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Cell Stem Cell
volume
1
issue
4
pages
428 - 442
publisher
Cell Press
external identifiers
  • wos:000251055300012
  • pmid:18371379
  • scopus:34848896359
ISSN
1934-5909
DOI
10.1016/j.stem.2007.07.005
language
English
LU publication?
yes
id
db7d7559-1e91-4498-ad69-ca365fcc85cd (old id 969203)
date added to LUP
2008-01-30 13:44:10
date last changed
2017-10-22 03:47:11
@article{db7d7559-1e91-4498-ad69-ca365fcc85cd,
  abstract     = {The major myeloid blood cell lineages are generated from hematopoietic stem cells by differentiation through a series of increasingly committed progenitor cells. Precise characterization of intermediate progenitors is important for understanding fundamental differentiation processes and a variety of disease states, including leukemia. Here, we evaluated the functional in vitro and in vivo potentials of a range of prospectively isolated myeloid precursors with differential expression of CD150, Endoglin, and CD41. Our studies revealed a hierarchy of myeloerythroid progenitors with distinct lineage potentials. The global gene expression signatures of these subsets were consistent with their functional capacities, and hierarchical clustering analysis suggested likely lineage relationships. These studies provide valuable tools for understanding myeloid lineage commitment, including isolation of an early erythroid-restricted precursor, and add to existing models of hematopoietic differentiation by suggesting that progenitors of the innate and adaptive immune system can separate late, following the divergence of megakaryocytic/erythroid potential.},
  author       = {Pronk, Cornelis J. H. and Rossi, Derrick J. and Månsson, Robert and Attema, Joanne and Norddahl, Gudmundur and Chan, Charles Kwok Fai and Sigvardsson, Mikael and Weissman, Irving L. and Bryder, David},
  issn         = {1934-5909},
  language     = {eng},
  number       = {4},
  pages        = {428--442},
  publisher    = {Cell Press},
  series       = {Cell Stem Cell},
  title        = {Elucidation of the phenotypic, functional, and molecular topography of a myeloerythroid progenitor cell hierarchy},
  url          = {http://dx.doi.org/10.1016/j.stem.2007.07.005},
  volume       = {1},
  year         = {2007},
}