Integrated on-chip system for DNA purification, labelling and surface stretching
(2018) 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 2. p.1177-1180- Abstract
We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/9718ac7e-6b26-4316-b850-06918e1a1663
- author
- Ström, Oskar E. LU ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU
- organization
- publishing date
- 2018
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- DNA labelling, DNA Purification, Molecular Combing
- host publication
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- series title
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- volume
- 2
- pages
- 4 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
- conference location
- Kaohsiung, Taiwan
- conference dates
- 2018-11-11 - 2018-11-15
- ISBN
- 9781510897571
- language
- English
- LU publication?
- yes
- id
- 9718ac7e-6b26-4316-b850-06918e1a1663
- date added to LUP
- 2020-03-13 15:53:07
- date last changed
- 2022-04-06 12:40:46
@inproceedings{9718ac7e-6b26-4316-b850-06918e1a1663, abstract = {{<p>We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.</p>}}, author = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}}, booktitle = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}}, isbn = {{9781510897571}}, keywords = {{DNA labelling; DNA Purification; Molecular Combing}}, language = {{eng}}, pages = {{1177--1180}}, publisher = {{Chemical and Biological Microsystems Society}}, series = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}}, title = {{Integrated on-chip system for DNA purification, labelling and surface stretching}}, volume = {{2}}, year = {{2018}}, }