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Integrated on-chip system for DNA purification, labelling and surface stretching

Ström, Oskar E. LU orcid ; Beech, Jason P. LU and Tegenfeldt, Jonas O. LU orcid (2018) 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018 2. p.1177-1180
Abstract

We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
DNA labelling, DNA Purification, Molecular Combing
host publication
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
series title
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
volume
2
pages
4 pages
publisher
Chemical and Biological Microsystems Society
conference name
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018
conference location
Kaohsiung, Taiwan
conference dates
2018-11-11 - 2018-11-15
ISBN
9781510897571
language
English
LU publication?
yes
id
9718ac7e-6b26-4316-b850-06918e1a1663
date added to LUP
2020-03-13 15:53:07
date last changed
2022-04-06 12:40:46
@inproceedings{9718ac7e-6b26-4316-b850-06918e1a1663,
  abstract     = {{<p>We report a simplified, integrated and more efficient way of preparing DNA for analysis. We have developed a microfluidic platform that can purify DNA and subsequently label and stretch it on a surface. We have successfully extracted DNA from a gel-filled microfluidic channel using electrophoresis and subsequently stretched the DNA linearly by absorption to either a hydrophobic or a positively-charged surface. To our knowledge, DNA has not previously been stretched on to a surface while being moved using an electric field in this way. We have also labelled DNA after stretching with intercalating dyes. The intention, following stretching and labelling, is to analyze the DNA with optical mapping.</p>}},
  author       = {{Ström, Oskar E. and Beech, Jason P. and Tegenfeldt, Jonas O.}},
  booktitle    = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}},
  isbn         = {{9781510897571}},
  keywords     = {{DNA labelling; DNA Purification; Molecular Combing}},
  language     = {{eng}},
  pages        = {{1177--1180}},
  publisher    = {{Chemical and Biological Microsystems Society}},
  series       = {{22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2018}},
  title        = {{Integrated on-chip system for DNA purification, labelling and surface stretching}},
  volume       = {{2}},
  year         = {{2018}},
}