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Regulation of the interferon-inducible p53 target gene TRIM22 (Staf50) in human T lymphocyte activation

Obad, Susanna LU ; Olofsson, Tor LU ; Mechti, Nadir; Gullberg, Urban LU and Drott, Kristina LU (2007) In Journal of Interferon and Cytokine Research 27(10). p.857-864
Abstract
TRIM22 (Staf50) is an interferon (IFN)-inducible protein with unknown function. Recently, we identified TRIM22 as a novel p53 target gene and showed that overexpression of TRIM22 inhibits the clonogenic growth of monoblastic U937 cells. Moreover, expression of TRIM22 is high in lymphoid tissue, and levels decrease during T lymphocyte activation with CD3/CD2/CD28, suggesting that TRIM22 could exert antiproliferative effects. Here, a prominent increase in TRIM22 levels is observed during activation with interleukin-2 (IL-2) or IL-15 in contrast to the decrease observed during CD3/CD2/CD28-induced activation. However, stimulation of cells in these experiments was performed on crude T lymphocytes, allowing indirect regulation between different... (More)
TRIM22 (Staf50) is an interferon (IFN)-inducible protein with unknown function. Recently, we identified TRIM22 as a novel p53 target gene and showed that overexpression of TRIM22 inhibits the clonogenic growth of monoblastic U937 cells. Moreover, expression of TRIM22 is high in lymphoid tissue, and levels decrease during T lymphocyte activation with CD3/CD2/CD28, suggesting that TRIM22 could exert antiproliferative effects. Here, a prominent increase in TRIM22 levels is observed during activation with interleukin-2 (IL-2) or IL-15 in contrast to the decrease observed during CD3/CD2/CD28-induced activation. However, stimulation of cells in these experiments was performed on crude T lymphocytes, allowing indirect regulation between different lymphocyte subtypes to take place. Therefore, to prevent interaction between different lymphocyte subtypes, expression of TRIM22 was examined during activation of sorted T lymphocyte subpopulations. In contrast to the marked changes of TRIM22 during activation of crude T lymphocytes, in isolated subpopulations, TRIM22 expression was not significantly affected in spite of IL-2-induced or CD3/CD2/CD28-induced activation. In addition, in contrast to the TRIM22 mouse ortholog Rpt-1, TRIM22 did not affect levels of CD25 (IL-2R alpha) mRNA. Our data suggest a more complex role for TRIM22 during T lymphocyte activation than merely as an antiproliferative factor. TRIM22 probably has an activation stage-specific role connected to the paracrine crosstalk during T lymphocyte activation. (Less)
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type
Contribution to journal
publication status
published
subject
in
Journal of Interferon and Cytokine Research
volume
27
issue
10
pages
857 - 864
publisher
Mary Ann Liebert, Inc.
external identifiers
  • wos:000250715100004
  • scopus:35648978234
ISSN
1079-9907
DOI
10.1089/jir.2006.0180
language
English
LU publication?
yes
id
9a009a55-15d5-4e3f-9196-7eaf38367eca (old id 974316)
date added to LUP
2008-01-30 07:29:03
date last changed
2017-09-17 07:01:11
@article{9a009a55-15d5-4e3f-9196-7eaf38367eca,
  abstract     = {TRIM22 (Staf50) is an interferon (IFN)-inducible protein with unknown function. Recently, we identified TRIM22 as a novel p53 target gene and showed that overexpression of TRIM22 inhibits the clonogenic growth of monoblastic U937 cells. Moreover, expression of TRIM22 is high in lymphoid tissue, and levels decrease during T lymphocyte activation with CD3/CD2/CD28, suggesting that TRIM22 could exert antiproliferative effects. Here, a prominent increase in TRIM22 levels is observed during activation with interleukin-2 (IL-2) or IL-15 in contrast to the decrease observed during CD3/CD2/CD28-induced activation. However, stimulation of cells in these experiments was performed on crude T lymphocytes, allowing indirect regulation between different lymphocyte subtypes to take place. Therefore, to prevent interaction between different lymphocyte subtypes, expression of TRIM22 was examined during activation of sorted T lymphocyte subpopulations. In contrast to the marked changes of TRIM22 during activation of crude T lymphocytes, in isolated subpopulations, TRIM22 expression was not significantly affected in spite of IL-2-induced or CD3/CD2/CD28-induced activation. In addition, in contrast to the TRIM22 mouse ortholog Rpt-1, TRIM22 did not affect levels of CD25 (IL-2R alpha) mRNA. Our data suggest a more complex role for TRIM22 during T lymphocyte activation than merely as an antiproliferative factor. TRIM22 probably has an activation stage-specific role connected to the paracrine crosstalk during T lymphocyte activation.},
  author       = {Obad, Susanna and Olofsson, Tor and Mechti, Nadir and Gullberg, Urban and Drott, Kristina},
  issn         = {1079-9907},
  language     = {eng},
  number       = {10},
  pages        = {857--864},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {Journal of Interferon and Cytokine Research},
  title        = {Regulation of the interferon-inducible p53 target gene TRIM22 (Staf50) in human T lymphocyte activation},
  url          = {http://dx.doi.org/10.1089/jir.2006.0180},
  volume       = {27},
  year         = {2007},
}