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Acanthamoeba-Campylobacter Coculture as a novel method for enrichment of Campylobacter species

Axelsson-Olsson, Diana; Ellstrom, Patrik; Waldenström, Jonas LU ; Haemig, Paul D.; Brudin, Lars and Olsen, Bjorn (2007) In Applied and Environmental Microbiology 73(21). p.6864-6869
Abstract
In this study, we present a novel method to isolate and enrich low concentrations of Campylobacter pathogens. This method, Acanthamoeba-Campylobacter coculture (ACC), is based on the intracellular survival and multiplication of Campylobacter species in the free-living protozoan Acanthamoeba polyphaga. Four of the Campylobacter species relevant to humans and livestock, Campylobacter jejuni, C. coli, C. lari, and C. hyointestinalis, were effectively enriched by the coculture method, with growth rates comparable to those observed in other Campylobacter enrichment media. Studying six strains of C.jejuni isolated from different sources, we found that all of the strains could be enriched from an inoculum of fewer than 10 bacteria. The... (More)
In this study, we present a novel method to isolate and enrich low concentrations of Campylobacter pathogens. This method, Acanthamoeba-Campylobacter coculture (ACC), is based on the intracellular survival and multiplication of Campylobacter species in the free-living protozoan Acanthamoeba polyphaga. Four of the Campylobacter species relevant to humans and livestock, Campylobacter jejuni, C. coli, C. lari, and C. hyointestinalis, were effectively enriched by the coculture method, with growth rates comparable to those observed in other Campylobacter enrichment media. Studying six strains of C.jejuni isolated from different sources, we found that all of the strains could be enriched from an inoculum of fewer than 10 bacteria. The sensitivity of the ACC method was not negatively affected by the use of Campylobacter-selective antibiotics in the culture medium, but these were effective in suppressing the growth of seven different bacterial species added at a concentration of 10(4) CFU/ml of each species as deliberate contamination. The ACC method has advantages over other enrichment methods as it is not dependent on a microaerobic milieu and does not require the use of blood or other oxygen-quenching agents. Our study found the ACC method to be a promising tool for the enrichment of Campylobacter species, particularly from water samples with low bacterial concentrations. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
73
issue
21
pages
6864 - 6869
publisher
American Society for Microbiology
external identifiers
  • wos:000250700600018
  • scopus:35948990875
ISSN
0099-2240
DOI
10.1128/AEM.01305-07
language
English
LU publication?
yes
id
0d59420b-73cd-4b27-8d79-8ad64d139c2a (old id 974435)
date added to LUP
2008-01-29 12:54:01
date last changed
2017-01-01 04:58:18
@article{0d59420b-73cd-4b27-8d79-8ad64d139c2a,
  abstract     = {In this study, we present a novel method to isolate and enrich low concentrations of Campylobacter pathogens. This method, Acanthamoeba-Campylobacter coculture (ACC), is based on the intracellular survival and multiplication of Campylobacter species in the free-living protozoan Acanthamoeba polyphaga. Four of the Campylobacter species relevant to humans and livestock, Campylobacter jejuni, C. coli, C. lari, and C. hyointestinalis, were effectively enriched by the coculture method, with growth rates comparable to those observed in other Campylobacter enrichment media. Studying six strains of C.jejuni isolated from different sources, we found that all of the strains could be enriched from an inoculum of fewer than 10 bacteria. The sensitivity of the ACC method was not negatively affected by the use of Campylobacter-selective antibiotics in the culture medium, but these were effective in suppressing the growth of seven different bacterial species added at a concentration of 10(4) CFU/ml of each species as deliberate contamination. The ACC method has advantages over other enrichment methods as it is not dependent on a microaerobic milieu and does not require the use of blood or other oxygen-quenching agents. Our study found the ACC method to be a promising tool for the enrichment of Campylobacter species, particularly from water samples with low bacterial concentrations.},
  author       = {Axelsson-Olsson, Diana and Ellstrom, Patrik and Waldenström, Jonas and Haemig, Paul D. and Brudin, Lars and Olsen, Bjorn},
  issn         = {0099-2240},
  language     = {eng},
  number       = {21},
  pages        = {6864--6869},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Acanthamoeba-Campylobacter Coculture as a novel method for enrichment of Campylobacter species},
  url          = {http://dx.doi.org/10.1128/AEM.01305-07},
  volume       = {73},
  year         = {2007},
}