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Lung Mast Cells Have a High Constitutive Expression of Carboxypeptidase A3 mRNA That Is Independent from Granule-Stored CPA3

Siddhuraj, Premkumar LU ; Clausson, Carl Magnus LU ; Sanden, Caroline LU ; Alyamani, Manar LU ; Kadivar, Mohammad LU ; Marsal, Jan LU ; Wallengren, Joanna LU orcid ; Bjermer, Leif LU and Erjefält, Jonas S. LU (2021) In Cells 10(2).
Abstract

The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on mRNA and protein expression at a single-cell level, the present study maps CPA3 mRNA and protein throughout the MCT and MCTC populations in healthy skin, gut and lung tissues. MCTC cells had both a higher frequency of CPA3 protein-containing cells and a higher protein-staining intensity than the MCT population. Among the tissues, skin MCs had highest CPA3 protein intensity. The expression pattern at the mRNA level was reversed. Lung mast cells had... (More)

The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on mRNA and protein expression at a single-cell level, the present study maps CPA3 mRNA and protein throughout the MCT and MCTC populations in healthy skin, gut and lung tissues. MCTC cells had both a higher frequency of CPA3 protein-containing cells and a higher protein-staining intensity than the MCT population. Among the tissues, skin MCs had highest CPA3 protein intensity. The expression pattern at the mRNA level was reversed. Lung mast cells had the highest mean CPA3 mRNA staining. Intriguingly, the large alveolar MCT population, that lack CPA3 protein, had uniquely high CPA3 mRNA intensity. A broader multi-tissue RNA analysis confirmed the uniquely high CPA3 mRNA quantities in the lung and corroborated the dissociation between chymase and CPA3 at the mRNA level. Taken together, our novel data suggest a hitherto underestimated contribution of mucosal-like MCT to baseline CPA3 mRNA production. The functional consequence of this high constitutive expression now reveals an important area for further research.

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author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
carboxypeptidase A3, chymase, gut, lung, mast cells, mRNA, skin, tryptase
in
Cells
volume
10
issue
2
publisher
MDPI AG
external identifiers
  • scopus:85101003035
  • pmid:33546258
ISSN
2073-4409
DOI
10.3390/cells10020309
language
English
LU publication?
yes
id
97673bd6-930b-49c9-9e5a-1b6f6898d87c
date added to LUP
2021-03-01 14:20:39
date last changed
2024-06-13 07:52:53
@article{97673bd6-930b-49c9-9e5a-1b6f6898d87c,
  abstract     = {{<p>The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on mRNA and protein expression at a single-cell level, the present study maps CPA3 mRNA and protein throughout the MCT and MCTC populations in healthy skin, gut and lung tissues. MCTC cells had both a higher frequency of CPA3 protein-containing cells and a higher protein-staining intensity than the MCT population. Among the tissues, skin MCs had highest CPA3 protein intensity. The expression pattern at the mRNA level was reversed. Lung mast cells had the highest mean CPA3 mRNA staining. Intriguingly, the large alveolar MCT population, that lack CPA3 protein, had uniquely high CPA3 mRNA intensity. A broader multi-tissue RNA analysis confirmed the uniquely high CPA3 mRNA quantities in the lung and corroborated the dissociation between chymase and CPA3 at the mRNA level. Taken together, our novel data suggest a hitherto underestimated contribution of mucosal-like MCT to baseline CPA3 mRNA production. The functional consequence of this high constitutive expression now reveals an important area for further research.</p>}},
  author       = {{Siddhuraj, Premkumar and Clausson, Carl Magnus and Sanden, Caroline and Alyamani, Manar and Kadivar, Mohammad and Marsal, Jan and Wallengren, Joanna and Bjermer, Leif and Erjefält, Jonas S.}},
  issn         = {{2073-4409}},
  keywords     = {{carboxypeptidase A3; chymase; gut; lung; mast cells; mRNA; skin; tryptase}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{MDPI AG}},
  series       = {{Cells}},
  title        = {{Lung Mast Cells Have a High Constitutive Expression of Carboxypeptidase A3 mRNA That Is Independent from Granule-Stored CPA3}},
  url          = {{http://dx.doi.org/10.3390/cells10020309}},
  doi          = {{10.3390/cells10020309}},
  volume       = {{10}},
  year         = {{2021}},
}