Direct monitoring of calcium-triggered phase transitions in cubosomes using small-angle X-ray scattering combined with microfluidics
Ghazal, Aghiad ; Gontsarik, Mark ; Kutter, Jörg P. ; Lafleur, Josiane P. ; Labrador, Ana LU
; Mortensen, Kell
and Yaghmur, Anan
(2016)
In Journal of Applied Crystallography
49(6).
p.2005-2014
- Abstract
This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The... (More)
This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The resulting SAXS data were resolved in the time frame between 0.5 and 5.5 s, and a calcium-triggered structural transition from an internal inverted-type cubic phase of symmetry Im3m to an internal inverted-type cubic phase of symmetry Pn3m was detected. The combination of microfluidics with X-ray techniques opens the door to the investigation of early dynamic structural transitions, which is not possible with conventional techniques such as glass flow cells. The combination of microfluidics with X-ray techniques can be used for investigating protein unfolding, for monitoring the formation of nanoparticles in real time, and for other biomedical and pharmaceutical investigations. A combination of microfluidics with X-ray techniques has been used to perform dynamic structural studies on nanoparticulate formulations.
(Less)
- author
- Ghazal, Aghiad
; Gontsarik, Mark
; Kutter, Jörg P.
; Lafleur, Josiane P.
; Labrador, Ana
LU
; Mortensen, Kell
and Yaghmur, Anan
- organization
- publishing date
- 2016-12-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- lipidic formulations, microfabrication, microfluidics, SAXS, small-angle X-ray scattering, X-ray techniques
- in
- Journal of Applied Crystallography
- volume
- 49
- issue
- 6
- pages
- 10 pages
- publisher
- International Union of Crystallography
- external identifiers
-
- wos:000391195900015
- scopus:85002989140
- ISSN
- 0021-8898
- DOI
- 10.1107/S1600576716014199
- language
- English
- LU publication?
- yes
- id
- 9794f20b-d9f8-47ee-88f3-30862a7f379d
- date added to LUP
- 2016-12-29 12:03:51
- date last changed
- 2025-03-08 23:10:28
@article{9794f20b-d9f8-47ee-88f3-30862a7f379d,
abstract = {{<p>This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The resulting SAXS data were resolved in the time frame between 0.5 and 5.5 s, and a calcium-triggered structural transition from an internal inverted-type cubic phase of symmetry Im3m to an internal inverted-type cubic phase of symmetry Pn3m was detected. The combination of microfluidics with X-ray techniques opens the door to the investigation of early dynamic structural transitions, which is not possible with conventional techniques such as glass flow cells. The combination of microfluidics with X-ray techniques can be used for investigating protein unfolding, for monitoring the formation of nanoparticles in real time, and for other biomedical and pharmaceutical investigations. A combination of microfluidics with X-ray techniques has been used to perform dynamic structural studies on nanoparticulate formulations.</p>}},
author = {{Ghazal, Aghiad and Gontsarik, Mark and Kutter, Jörg P. and Lafleur, Josiane P. and Labrador, Ana and Mortensen, Kell and Yaghmur, Anan}},
issn = {{0021-8898}},
keywords = {{lipidic formulations; microfabrication; microfluidics; SAXS; small-angle X-ray scattering; X-ray techniques}},
language = {{eng}},
month = {{12}},
number = {{6}},
pages = {{2005--2014}},
publisher = {{International Union of Crystallography}},
series = {{Journal of Applied Crystallography}},
title = {{Direct monitoring of calcium-triggered phase transitions in cubosomes using small-angle X-ray scattering combined with microfluidics}},
url = {{http://dx.doi.org/10.1107/S1600576716014199}},
doi = {{10.1107/S1600576716014199}},
volume = {{49}},
year = {{2016}},
}