Advanced

Direct monitoring of calcium-triggered phase transitions in cubosomes using small-angle X-ray scattering combined with microfluidics

Ghazal, Aghiad; Gontsarik, Mark; Kutter, Jörg P.; Lafleur, Josiane P.; Labrador, Ana LU ; Mortensen, Kell and Yaghmur, Anan (2016) In Journal of Applied Crystallography 49(6). p.2005-2014
Abstract

This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The... (More)

This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The resulting SAXS data were resolved in the time frame between 0.5 and 5.5 s, and a calcium-triggered structural transition from an internal inverted-type cubic phase of symmetry Im3m to an internal inverted-type cubic phase of symmetry Pn3m was detected. The combination of microfluidics with X-ray techniques opens the door to the investigation of early dynamic structural transitions, which is not possible with conventional techniques such as glass flow cells. The combination of microfluidics with X-ray techniques can be used for investigating protein unfolding, for monitoring the formation of nanoparticles in real time, and for other biomedical and pharmaceutical investigations. A combination of microfluidics with X-ray techniques has been used to perform dynamic structural studies on nanoparticulate formulations.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
lipidic formulations, microfabrication, microfluidics, SAXS, small-angle X-ray scattering, X-ray techniques
in
Journal of Applied Crystallography
volume
49
issue
6
pages
10 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:85002989140
  • wos:000391195900015
ISSN
0021-8898
DOI
10.1107/S1600576716014199
language
English
LU publication?
yes
id
9794f20b-d9f8-47ee-88f3-30862a7f379d
date added to LUP
2016-12-29 12:03:51
date last changed
2017-10-01 05:28:16
@article{9794f20b-d9f8-47ee-88f3-30862a7f379d,
  abstract     = {<p>This article introduces a simple microfluidic device that can be combined with synchrotron small-angle X-ray scattering (SAXS) for monitoring dynamic structural transitions. The microfluidic device is a thiol-ene-based system equipped with 125 μm-thick polystyrene windows, which are suitable for X-ray experiments. The device was prepared by soft lithography using elastomeric molds followed by a simple UV-initiated curing step to polymerize the chip material and simultaneously seal the device with the polystyrene windows. The microfluidic device was successfully used to explore the dynamics of the structural transitions of phytantriol/dioleoylphosphatidylglycerol-based cubosomes on exposure to a buffer containing calcium ions. The resulting SAXS data were resolved in the time frame between 0.5 and 5.5 s, and a calcium-triggered structural transition from an internal inverted-type cubic phase of symmetry Im3m to an internal inverted-type cubic phase of symmetry Pn3m was detected. The combination of microfluidics with X-ray techniques opens the door to the investigation of early dynamic structural transitions, which is not possible with conventional techniques such as glass flow cells. The combination of microfluidics with X-ray techniques can be used for investigating protein unfolding, for monitoring the formation of nanoparticles in real time, and for other biomedical and pharmaceutical investigations. A combination of microfluidics with X-ray techniques has been used to perform dynamic structural studies on nanoparticulate formulations.</p>},
  author       = {Ghazal, Aghiad and Gontsarik, Mark and Kutter, Jörg P. and Lafleur, Josiane P. and Labrador, Ana and Mortensen, Kell and Yaghmur, Anan},
  issn         = {0021-8898},
  keyword      = {lipidic formulations,microfabrication,microfluidics,SAXS,small-angle X-ray scattering,X-ray techniques},
  language     = {eng},
  month        = {12},
  number       = {6},
  pages        = {2005--2014},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Applied Crystallography},
  title        = {Direct monitoring of calcium-triggered phase transitions in cubosomes using small-angle X-ray scattering combined with microfluidics},
  url          = {http://dx.doi.org/10.1107/S1600576716014199},
  volume       = {49},
  year         = {2016},
}