Differentially amplified chromosome 12 sequences in low- and high-grade osteosarcoma.
(2002) In Genes, Chromosomes and Cancer 33(2). p.133-140- Abstract
- Most osteosarcomas are highly aggressive malignancies characterized by a complex pattern of chromosome abnormalities. However, a subgroup of low-grade, parosteal tumors exhibits a relatively simple aberration pattern dominated by ring chromosomes carrying amplified material from chromosome 12. To assess whether sequences from this chromosome were differentially amplified in low- and high-grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 regions in 12p and the MDM2 region in 12q were evaluated by interphase or metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. Amplification of MDM2 was detected in all five low-grade and four high-grade osteosarcomas, all of which showed ring chromosomes. An... (More)
- Most osteosarcomas are highly aggressive malignancies characterized by a complex pattern of chromosome abnormalities. However, a subgroup of low-grade, parosteal tumors exhibits a relatively simple aberration pattern dominated by ring chromosomes carrying amplified material from chromosome 12. To assess whether sequences from this chromosome were differentially amplified in low- and high-grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 regions in 12p and the MDM2 region in 12q were evaluated by interphase or metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. Amplification of MDM2 was detected in all five low-grade and four high-grade osteosarcomas, all of which showed ring chromosomes. An overrepresentation of 12p sequences was found in 1/5 low-grade and in 9/19 high-grade tumors. Multicolor single-copy FISH analysis of metaphase cells from six high-grade tumors showed that extra 12p material either occurred together with MDM2 in ring chromosomes or was scattered over the genome as a result of complex structural rearrangements. Most tumors (8/10) not containing amplification of the assessed chromosome 12 loci exhibited a nondiploid pattern at evaluation with probes for centromeric alpha satellite sequences. These findings indicate that gain of sequences from the short arm of chromosome 12 could be a possible genetic pathway in the development of aggressive osteosarcoma. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/106634
- author
- Gisselsson Nord, David LU ; Pålsson, Eva LU ; Höglund, Mattias LU ; Domanski, Henryk LU ; Mertens, Fredrik LU ; Pandis, Nikos ; Sciot, Raf ; Dal Cin, Paola ; Bridge, Julia A and Mandahl, Nils LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Karyotyping, Interphase/genetics, Fluorescence, In Situ Hybridization, Human, Gene Amplification/*genetics, Female, Comparative Study, Pair 12/*genetics, Chromosomes, Chromosome Banding, Child, Bone Neoplasms/*genetics/pathology, Base Sequence, Adolescence, Adult, Male, Metaphase/genetics, Middle Age, Osteosarcoma/*genetics/pathology, Support, Non-U.S. Gov't, Tumor Cells, Cultured
- in
- Genes, Chromosomes and Cancer
- volume
- 33
- issue
- 2
- pages
- 133 - 140
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- wos:000173107000004
- pmid:11793439
- scopus:18244383816
- ISSN
- 1045-2257
- DOI
- 10.1002/gcc.1219
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Division of Clinical Genetics (013022003)
- id
- 97b7c643-cbfc-44fe-aca2-0b15f8e896c5 (old id 106634)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11793439&dopt=Abstract
- date added to LUP
- 2016-04-01 12:01:39
- date last changed
- 2022-03-13 04:11:59
@article{97b7c643-cbfc-44fe-aca2-0b15f8e896c5,
abstract = {{Most osteosarcomas are highly aggressive malignancies characterized by a complex pattern of chromosome abnormalities. However, a subgroup of low-grade, parosteal tumors exhibits a relatively simple aberration pattern dominated by ring chromosomes carrying amplified material from chromosome 12. To assess whether sequences from this chromosome were differentially amplified in low- and high-grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 regions in 12p and the MDM2 region in 12q were evaluated by interphase or metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. Amplification of MDM2 was detected in all five low-grade and four high-grade osteosarcomas, all of which showed ring chromosomes. An overrepresentation of 12p sequences was found in 1/5 low-grade and in 9/19 high-grade tumors. Multicolor single-copy FISH analysis of metaphase cells from six high-grade tumors showed that extra 12p material either occurred together with MDM2 in ring chromosomes or was scattered over the genome as a result of complex structural rearrangements. Most tumors (8/10) not containing amplification of the assessed chromosome 12 loci exhibited a nondiploid pattern at evaluation with probes for centromeric alpha satellite sequences. These findings indicate that gain of sequences from the short arm of chromosome 12 could be a possible genetic pathway in the development of aggressive osteosarcoma.}},
author = {{Gisselsson Nord, David and Pålsson, Eva and Höglund, Mattias and Domanski, Henryk and Mertens, Fredrik and Pandis, Nikos and Sciot, Raf and Dal Cin, Paola and Bridge, Julia A and Mandahl, Nils}},
issn = {{1045-2257}},
keywords = {{Karyotyping; Interphase/genetics; Fluorescence; In Situ Hybridization; Human; Gene Amplification/*genetics; Female; Comparative Study; Pair 12/*genetics; Chromosomes; Chromosome Banding; Child; Bone Neoplasms/*genetics/pathology; Base Sequence; Adolescence; Adult; Male; Metaphase/genetics; Middle Age; Osteosarcoma/*genetics/pathology; Support; Non-U.S. Gov't; Tumor Cells; Cultured}},
language = {{eng}},
number = {{2}},
pages = {{133--140}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Genes, Chromosomes and Cancer}},
title = {{Differentially amplified chromosome 12 sequences in low- and high-grade osteosarcoma.}},
url = {{http://dx.doi.org/10.1002/gcc.1219}},
doi = {{10.1002/gcc.1219}},
volume = {{33}},
year = {{2002}},
}