Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria
(1996) In Plant Physiology 112(1). p.75-78- Abstract
The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was... (More)
The addition of ubiquinone-1 (UQ-1) induced Ca2+-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD+-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.
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- author
- Møller, Ian M. ; Roberts, Thomas H. and Rasmusson, Allan G. LU
- organization
- publishing date
- 1996-09
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Plant Physiology
- volume
- 112
- issue
- 1
- pages
- 4 pages
- publisher
- American Society of Plant Biologists
- external identifiers
-
- scopus:0029911102
- ISSN
- 0032-0889
- language
- English
- LU publication?
- yes
- id
- 97f7d801-5855-42d4-9c7b-65619637cc08
- alternative location
- http://www.plantphysiol.org/content/112/1/75.full.pdf+html
- date added to LUP
- 2016-05-31 21:33:23
- date last changed
- 2024-01-04 07:46:39
@article{97f7d801-5855-42d4-9c7b-65619637cc08, abstract = {{<p>The addition of ubiquinone-1 (UQ-1) induced Ca<sup>2+</sup>-independent oxidation of deamino-NADH and NADH by intact potato (Solanum tuberosum L. cv Bintje) tuber mitochondria. The induced oxidation was coupled to the generation of a membrane potential. Measurements of NAD<sup>+</sup>-malate dehydrogenase activity indicated that the permeability of the inner mitochondrial membrane to NADH and deamino-NADH was not altered by the addition of UQ-1. We conclude that UQ-1-induced external deamino-NADH oxidation is due to a change in specificity of the external rotenone-insensitive NADH dehydrogenase. The addition of UQ-1 also induced rotenone-insensitive oxidation of deamino-NADH by inside-out submitochondrial particles, but whether this was due to a change in the specificity of the internal rotenone-insensitive NAD(P)H dehydrogenase or to a bypass in complex I could not be determined.</p>}}, author = {{Møller, Ian M. and Roberts, Thomas H. and Rasmusson, Allan G.}}, issn = {{0032-0889}}, language = {{eng}}, number = {{1}}, pages = {{75--78}}, publisher = {{American Society of Plant Biologists}}, series = {{Plant Physiology}}, title = {{Ubiquinone-1 induces external deamino-NADH oxidation in potato tuber mitochondria}}, url = {{http://www.plantphysiol.org/content/112/1/75.full.pdf+html}}, volume = {{112}}, year = {{1996}}, }