Conserved sequence elements in K promoters from mice and humans : Implications for transcriptional regulation and repertoire expression
(1998) In Immunogenetics 47(3). p.183-195- Abstract
Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were... (More)
Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V families. We conclude from this study that conservation of additional sequence elements besides the octamer is a common feature in Igk-V promoters but that distinct elements are conserved only within a given subgroup/family. Thus, the conservation appears to have operated at the level of function rather than at the level of recognition sequence for defined transcription factors.
(Less)
- author
- Bemark, Mats LU ; Liberg, David LU and Leanderson, Tomas LU
- organization
- publishing date
- 1998
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- E-box, Immunoglobulin, Octamer, Promoter, Transcription
- in
- Immunogenetics
- volume
- 47
- issue
- 3
- pages
- 183 - 195
- publisher
- Springer
- external identifiers
-
- pmid:9435336
- scopus:0031975839
- ISSN
- 0093-7711
- DOI
- 10.1007/s002510050347
- language
- English
- LU publication?
- yes
- id
- 98692b45-8a9b-4b4c-8249-f8715b050d94
- date added to LUP
- 2023-12-06 16:42:30
- date last changed
- 2024-03-07 14:43:08
@article{98692b45-8a9b-4b4c-8249-f8715b050d94, abstract = {{<p>Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V families. We conclude from this study that conservation of additional sequence elements besides the octamer is a common feature in Igk-V promoters but that distinct elements are conserved only within a given subgroup/family. Thus, the conservation appears to have operated at the level of function rather than at the level of recognition sequence for defined transcription factors.</p>}}, author = {{Bemark, Mats and Liberg, David and Leanderson, Tomas}}, issn = {{0093-7711}}, keywords = {{E-box; Immunoglobulin; Octamer; Promoter; Transcription}}, language = {{eng}}, number = {{3}}, pages = {{183--195}}, publisher = {{Springer}}, series = {{Immunogenetics}}, title = {{Conserved sequence elements in K promoters from mice and humans : Implications for transcriptional regulation and repertoire expression}}, url = {{http://dx.doi.org/10.1007/s002510050347}}, doi = {{10.1007/s002510050347}}, volume = {{47}}, year = {{1998}}, }