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Conserved sequence elements in K promoters from mice and humans : Implications for transcriptional regulation and repertoire expression

Bemark, Mats LU orcid ; Liberg, David LU and Leanderson, Tomas LU (1998) In Immunogenetics 47(3). p.183-195
Abstract

Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were... (More)

Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V families. We conclude from this study that conservation of additional sequence elements besides the octamer is a common feature in Igk-V promoters but that distinct elements are conserved only within a given subgroup/family. Thus, the conservation appears to have operated at the level of function rather than at the level of recognition sequence for defined transcription factors.

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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
E-box, Immunoglobulin, Octamer, Promoter, Transcription
in
Immunogenetics
volume
47
issue
3
pages
183 - 195
publisher
Springer
external identifiers
  • pmid:9435336
  • scopus:0031975839
ISSN
0093-7711
DOI
10.1007/s002510050347
language
English
LU publication?
yes
id
98692b45-8a9b-4b4c-8249-f8715b050d94
date added to LUP
2023-12-06 16:42:30
date last changed
2024-03-07 14:43:08
@article{98692b45-8a9b-4b4c-8249-f8715b050d94,
  abstract     = {{<p>Promoter region sequences of human and mouse Igk-V genes were aligned and found to be conserved for about 200-300 base pairs (bp) within subgroups/families. No promoter similarity was found between IGKV promoters from different human subgroups. Related mouse Igk-V gene families were conserved in the promoter region but no similarity was evident when promoters from unrelated Igk-V gene families were compared. Most of the human IGKV promoter subgroups were shown to have mouse counterparts with a similarity region that extended about 150 bp upstream of the translational start codon. All promoters contained an octamer sequence element. The consensus octamer/decamer sequence was favored but only seven residues within the octamer element were strictly conserved. Furthermore, there was also sequence conservation immediately 3' of the octamer where either an A or a G residue was conserved. In addition, other DNA elements were also conserved both within the Igk-V subgroups/families and between mouse and human promoters from related subgroups/families. In several of the subgroups/families an E box of the E2A type was conserved 5' of the octamer and a CCCT element was conserved within the IGKV subgroup II and its related mouse Igk-V families. We conclude from this study that conservation of additional sequence elements besides the octamer is a common feature in Igk-V promoters but that distinct elements are conserved only within a given subgroup/family. Thus, the conservation appears to have operated at the level of function rather than at the level of recognition sequence for defined transcription factors.</p>}},
  author       = {{Bemark, Mats and Liberg, David and Leanderson, Tomas}},
  issn         = {{0093-7711}},
  keywords     = {{E-box; Immunoglobulin; Octamer; Promoter; Transcription}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{183--195}},
  publisher    = {{Springer}},
  series       = {{Immunogenetics}},
  title        = {{Conserved sequence elements in K promoters from mice and humans : Implications for transcriptional regulation and repertoire expression}},
  url          = {{http://dx.doi.org/10.1007/s002510050347}},
  doi          = {{10.1007/s002510050347}},
  volume       = {{47}},
  year         = {{1998}},
}