A generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures
Marie, Rodolphe LU ; Dahlin, Andreas LU ; Tegenfeldt, Jonas LU
and Höök, Fredrik
LU
(2007)
In Biointerphases
2(1).
p.49-55
- Abstract
- A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the... (More)
- A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. (C) 2007 American Vacuum Society. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/988404
- author
- Marie, Rodolphe
LU
; Dahlin, Andreas
LU
; Tegenfeldt, Jonas
LU
and Höök, Fredrik
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biointerphases
- volume
- 2
- issue
- 1
- pages
- 49 - 55
- publisher
- American Institute of Physics (AIP)
- external identifiers
-
- wos:000256466800007
- ISSN
- 1934-8630
- DOI
- 10.1116/1.2717926
- language
- English
- LU publication?
- yes
- id
- bf4f3f76-8184-4e2e-83d9-18b6c3050794 (old id 988404)
- date added to LUP
- 2016-04-01 12:38:39
- date last changed
- 2024-08-20 11:37:39
@article{bf4f3f76-8184-4e2e-83d9-18b6c3050794,
abstract = {{A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. (C) 2007 American Vacuum Society.}},
author = {{Marie, Rodolphe and Dahlin, Andreas and Tegenfeldt, Jonas and Höök, Fredrik}},
issn = {{1934-8630}},
language = {{eng}},
number = {{1}},
pages = {{49--55}},
publisher = {{American Institute of Physics (AIP)}},
series = {{Biointerphases}},
title = {{A generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures}},
url = {{http://dx.doi.org/10.1116/1.2717926}},
doi = {{10.1116/1.2717926}},
volume = {{2}},
year = {{2007}},
}