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A generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures

Marie, Rodolphe LU ; Dahlin, Andreas LU ; Tegenfeldt, Jonas LU and Höök, Fredrik LU (2007) In Biointerphases 2(1). p.49-55
Abstract
A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the... (More)
A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. (C) 2007 American Vacuum Society. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biointerphases
volume
2
issue
1
pages
49 - 55
publisher
AVS
external identifiers
  • wos:000256466800007
ISSN
1934-8630
DOI
10.1116/1.2717926
language
English
LU publication?
yes
id
bf4f3f76-8184-4e2e-83d9-18b6c3050794 (old id 988404)
date added to LUP
2008-01-29 12:00:13
date last changed
2016-04-15 20:49:35
@article{bf4f3f76-8184-4e2e-83d9-18b6c3050794,
  abstract     = {A generic protocol for the creation of material-mediated self-assembled patterns of streptavidin, defined solely by patterns of gold and SiO2, is presented. Protein-adsorption resistance of selected regions was obtained by material-specific adsorption of thiol-modified poly(ethylene)glycol (thiol-PEG) on gold followed by adsorption of poly-L-lysine (PLL) modified PEG (PLL-g-PEG) on SiO2. Selective streptavidin binding to either gold or SiO2 (or both) was ensured by introducing biotin-modified thiolated (thiol-biotin) and/or biotin-modified PLL-g-PEG (PLL-g-PEGbiotin) compounds. The introduction of biotin did not influence the protein-adsorption resistance. On the macroscopic scale, the protein-adsorption-resistant properties and the streptavidin-binding capacity were optimized using quartz crystal microbalance with dissipation monitoring. The reproduction of micrometer-scale gold patterns on SiO2 into patterns of streptavidin was verified using fluorescence microscopy, while the compatibility of the material-specific surface-modification strategy with nanoscale features was accomplished by modifying a localized surface plasmon resonance (LSPR) active template, defined by randomly distributed nanoapertures in a thin gold film on SiO2. The demonstrated compatibility of the latter substrate with LSPR-based label-free sensing of biorecognition reactions, combined with the fact that all compounds utilized are commercially available, makes the surface-modification protocol attractive as a generic surface modification solution for a broad range of biorecognition-based assays. (C) 2007 American Vacuum Society.},
  author       = {Marie, Rodolphe and Dahlin, Andreas and Tegenfeldt, Jonas and Höök, Fredrik},
  issn         = {1934-8630},
  language     = {eng},
  number       = {1},
  pages        = {49--55},
  publisher    = {AVS},
  series       = {Biointerphases},
  title        = {A generic surface modification strategy for sensing applications based on Au/SiO2 nanostructures},
  url          = {http://dx.doi.org/10.1116/1.2717926},
  volume       = {2},
  year         = {2007},
}