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Implementation of a multiplexed structured illumination method to achieve snapshot multispectral imaging

Dorozynska, Karolina LU and Kristensson, Elias LU (2017) In Optics Express 25(15). p.17211-17226
Abstract

An instantaneous multispectral imaging setup based on frequency recognition algorithm for multiple exposures (FRAME) is presented and demonstrated experimentally. With this implementation of FRAME, each light source is uniquely encoded with a spatial modulation and the corresponding fluorescent responses pertaining to each maintain the same unique encoding. This allows the extraction of each source response from a single captured image by filtering in the Fourier domain. As a result, a multispectral imaging system based on FRAME can perform all the illumination and corresponding fluorescence detection simultaneously, where the latter is recorded in a single exposure and on a single detector and is thus capable of recording true... (More)

An instantaneous multispectral imaging setup based on frequency recognition algorithm for multiple exposures (FRAME) is presented and demonstrated experimentally. With this implementation of FRAME, each light source is uniquely encoded with a spatial modulation and the corresponding fluorescent responses pertaining to each maintain the same unique encoding. This allows the extraction of each source response from a single captured image by filtering in the Fourier domain. As a result, a multispectral imaging system based on FRAME can perform all the illumination and corresponding fluorescence detection simultaneously, where the latter is recorded in a single exposure and on a single detector and is thus capable of recording true ‘snapshot’ multispectral images. The results presented here demonstrate that the technique is capable of distinguishing source responses for well separated and co-localized fluorophores as well as providing z-sectioning capabilities. This implementation of FRAME demonstrates its viability as a tool for multispectral imaging of dynamic samples. Additionally, since all the spectral images are captured simultaneously, the method has potential for studying samples prone to photobleaching. Finally, this application of FRAME makes it possible to discriminate between signals due to infinitely spectrally close sources which, to the best of the authors’ knowledge, has not been possible in snapshot multispectral imaging schemes before.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Optics Express
volume
25
issue
15
pages
16 pages
publisher
OSA
external identifiers
  • scopus:85025839155
  • wos:000408584400028
ISSN
1094-4087
DOI
10.1364/OE.25.017211
language
English
LU publication?
yes
id
993e2b06-c274-4919-92c2-6ebcf4bedbf0
date added to LUP
2017-08-02 07:44:58
date last changed
2017-09-18 11:40:44
@article{993e2b06-c274-4919-92c2-6ebcf4bedbf0,
  abstract     = {<p>An instantaneous multispectral imaging setup based on frequency recognition algorithm for multiple exposures (FRAME) is presented and demonstrated experimentally. With this implementation of FRAME, each light source is uniquely encoded with a spatial modulation and the corresponding fluorescent responses pertaining to each maintain the same unique encoding. This allows the extraction of each source response from a single captured image by filtering in the Fourier domain. As a result, a multispectral imaging system based on FRAME can perform all the illumination and corresponding fluorescence detection simultaneously, where the latter is recorded in a single exposure and on a single detector and is thus capable of recording true ‘snapshot’ multispectral images. The results presented here demonstrate that the technique is capable of distinguishing source responses for well separated and co-localized fluorophores as well as providing z-sectioning capabilities. This implementation of FRAME demonstrates its viability as a tool for multispectral imaging of dynamic samples. Additionally, since all the spectral images are captured simultaneously, the method has potential for studying samples prone to photobleaching. Finally, this application of FRAME makes it possible to discriminate between signals due to infinitely spectrally close sources which, to the best of the authors’ knowledge, has not been possible in snapshot multispectral imaging schemes before.</p>},
  author       = {Dorozynska, Karolina and Kristensson, Elias},
  issn         = {1094-4087},
  language     = {eng},
  month        = {07},
  number       = {15},
  pages        = {17211--17226},
  publisher    = {OSA},
  series       = {Optics Express},
  title        = {Implementation of a multiplexed structured illumination method to achieve snapshot multispectral imaging},
  url          = {http://dx.doi.org/10.1364/OE.25.017211},
  volume       = {25},
  year         = {2017},
}