Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Investigating the Influence of a Tooth Absence on Facial Bone Growth Using a Porcine Model

Szkopek, Dominika ; Wychowański, Piotr ; Zaworski, Kamil LU ; Seklecka, Blanka ; Starzyński, Rafał ; Lipiński, Paweł ; Pierzynowska, Kateryna LU orcid ; Pierzynowski, Stefan G. LU ; Donaldson, Janine and Paczewski, Łukasz , et al. (2024) In International Journal of Molecular Sciences 25(23).
Abstract

With the current state of knowledge regarding disorders of facial bone development, including anodontia, the development of a suitable animal model for preclinical studies is essential. The agenesis of dental buds occurs in about 25% of the human population. Prospects for treatment include the use of growth factors, stem cells, and bioengineering. This study aimed to investigate the influence of a tooth absence on facial bone growth, develop a technique for the application of growth factors to the developing bone, and analyze the comparative effect of the application of selected active proteins on the growth of the maxilla and mandible. Piglets underwent germectomy, followed by computed tomography and X-ray; morphometric and... (More)

With the current state of knowledge regarding disorders of facial bone development, including anodontia, the development of a suitable animal model for preclinical studies is essential. The agenesis of dental buds occurs in about 25% of the human population. Prospects for treatment include the use of growth factors, stem cells, and bioengineering. This study aimed to investigate the influence of a tooth absence on facial bone growth, develop a technique for the application of growth factors to the developing bone, and analyze the comparative effect of the application of selected active proteins on the growth of the maxilla and mandible. Piglets underwent germectomy, followed by computed tomography and X-ray; morphometric and histological analyses of the bones were performed, blood bone morphogenetic protein 2 and platelet-derived growth factor concentrations were determined, and the transcriptomic profile of the dentate ligament was analyzed using DNA microarrays. It was not possible to identify the most effective growth factor application algorithm for achieving normal jaw development. Normal mandibular bone structure and oral mucosa structure were observed in the germectomy groups with growth factor augmentation. The average height of the mandibular alveolar part in the area of the removed dental buds was significantly lower compared with that of the inoperable side, 3 months after surgery. However, no significant differences were found in the serum concentrations of BMP-2 and PDGF between groups. The animal model of bone development disorders (including anodontia) developed in the current study and the scheme for evaluating the efficacy and safety of the application of replacement therapy for craniofacial malformations are important in the development of the discipline and represent an important contribution to the introduction of treatment methods.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; ; and , et al. (More)
; ; ; ; ; ; ; ; ; and (Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
animal model, anodontia, bone growth development disorders, growth factors, tooth buds
in
International Journal of Molecular Sciences
volume
25
issue
23
article number
12509
publisher
MDPI AG
external identifiers
  • pmid:39684221
  • scopus:85211823613
ISSN
1661-6596
DOI
10.3390/ijms252312509
language
English
LU publication?
yes
id
997c3fa6-3e36-4514-a8fc-6ba9e747fcbc
date added to LUP
2025-01-22 11:28:54
date last changed
2025-06-11 22:17:41
@article{997c3fa6-3e36-4514-a8fc-6ba9e747fcbc,
  abstract     = {{<p>With the current state of knowledge regarding disorders of facial bone development, including anodontia, the development of a suitable animal model for preclinical studies is essential. The agenesis of dental buds occurs in about 25% of the human population. Prospects for treatment include the use of growth factors, stem cells, and bioengineering. This study aimed to investigate the influence of a tooth absence on facial bone growth, develop a technique for the application of growth factors to the developing bone, and analyze the comparative effect of the application of selected active proteins on the growth of the maxilla and mandible. Piglets underwent germectomy, followed by computed tomography and X-ray; morphometric and histological analyses of the bones were performed, blood bone morphogenetic protein 2 and platelet-derived growth factor concentrations were determined, and the transcriptomic profile of the dentate ligament was analyzed using DNA microarrays. It was not possible to identify the most effective growth factor application algorithm for achieving normal jaw development. Normal mandibular bone structure and oral mucosa structure were observed in the germectomy groups with growth factor augmentation. The average height of the mandibular alveolar part in the area of the removed dental buds was significantly lower compared with that of the inoperable side, 3 months after surgery. However, no significant differences were found in the serum concentrations of BMP-2 and PDGF between groups. The animal model of bone development disorders (including anodontia) developed in the current study and the scheme for evaluating the efficacy and safety of the application of replacement therapy for craniofacial malformations are important in the development of the discipline and represent an important contribution to the introduction of treatment methods.</p>}},
  author       = {{Szkopek, Dominika and Wychowański, Piotr and Zaworski, Kamil and Seklecka, Blanka and Starzyński, Rafał and Lipiński, Paweł and Pierzynowska, Kateryna and Pierzynowski, Stefan G. and Donaldson, Janine and Paczewski, Łukasz and Woliński, Jarosław}},
  issn         = {{1661-6596}},
  keywords     = {{animal model; anodontia; bone growth development disorders; growth factors; tooth buds}},
  language     = {{eng}},
  number       = {{23}},
  publisher    = {{MDPI AG}},
  series       = {{International Journal of Molecular Sciences}},
  title        = {{Investigating the Influence of a Tooth Absence on Facial Bone Growth Using a Porcine Model}},
  url          = {{http://dx.doi.org/10.3390/ijms252312509}},
  doi          = {{10.3390/ijms252312509}},
  volume       = {{25}},
  year         = {{2024}},
}