A Probing Feeding Strategy for Escherichia coli Cultures
(1999) In Biotechnology Techniques 13. p.523-528- Abstract
- A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8497617
- author
- Axelsson, Jan Peter ; Åkesson, Mats LU and Hagander, Per LU
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Escherichia coli, fed-batch culture, glucose, acetate, feedback control, dissolved oxygen.
- in
- Biotechnology Techniques
- volume
- 13
- pages
- 523 - 528
- publisher
- Springer
- external identifiers
-
- scopus:0032883459
- ISSN
- 0951-208X
- language
- English
- LU publication?
- yes
- id
- 99cc0e2d-5c9d-4926-b8a4-f1653b98082e (old id 8497617)
- date added to LUP
- 2016-04-04 14:34:31
- date last changed
- 2022-01-30 02:11:54
@article{99cc0e2d-5c9d-4926-b8a4-f1653b98082e, abstract = {{A strain-independent feeding strategy for fed-batch cultures of <em>Escherichia coli</em> is presented. The key idea is to exploit a characteristic change in the cell metabolism at the onset of acetate formation. By superimposing short pulses in the glucose feed rate, on-line detection of acetate formation can be made using a standard dissolved oxygen sensor. A simple feedback algorithm is then used to adjust the feed rate to avoid acetate formation. The feasibility of the strategy is demonstrated by both simulation and experiments.}}, author = {{Axelsson, Jan Peter and Åkesson, Mats and Hagander, Per}}, issn = {{0951-208X}}, keywords = {{Escherichia coli; fed-batch culture; glucose; acetate; feedback control; dissolved oxygen.}}, language = {{eng}}, pages = {{523--528}}, publisher = {{Springer}}, series = {{Biotechnology Techniques}}, title = {{A Probing Feeding Strategy for <em>Escherichia coli</em> Cultures}}, volume = {{13}}, year = {{1999}}, }