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Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa

Avalos Redón, I ; Más Lago, P J ; Sarmiento Pérez, L R LU ; Palomera Puente, R ; Muné Jiménez, M ; Bello Corredor, M and Pérez Santos, L (1998) In Revista cubana de medicina tropical 50(2). p.4-100
Abstract

The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about... (More)

The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.

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author
; ; ; ; ; and
alternative title
The intratypic characterization of the poliovirus by the polymerase chain reaction technic
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Base Sequence, Humans, Molecular Sequence Data, Oligonucleotide Probes, Poliovirus/classification, Polymerase Chain Reaction/methods, RNA, Viral/genetics
in
Revista cubana de medicina tropical
volume
50
issue
2
pages
5 pages
publisher
Editorial Ciencias Medicas
external identifiers
  • pmid:10349426
  • scopus:0032249807
ISSN
0375-0760
language
Spanish
LU publication?
no
id
99d6a68e-2534-422b-895b-4e3d7f8f8077
alternative location
http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601998000200004
date added to LUP
2019-07-08 15:18:47
date last changed
2020-01-13 02:12:31
@article{99d6a68e-2534-422b-895b-4e3d7f8f8077,
  abstract     = {<p>The polymerase chain reaction techniques was introduced for the intratypic characterization of Poliovirus. Primers were used only to promote the amplification of the Sabin vaccine strains proved by electrophoretic run of the amplified DNA products (Sabin 1-97 pb, Sabin 2-71 pb, Sabin 3-44 pb) and whose specificity was satisfactorily verified. 23 Cuban poliovirus strains isolated and identified at the Laboratory of Enterovirus of the "Pedro Kourí" Tropical Medicine Institute from 1993 to 1994 were studied by this technique. All of them were of the vaccine type. It was observed how the Sabin vaccine poliovirus may be the cause of viral meningoencephalitis as a milder neurological complication. This study provided one more evidence about the non circulation of the wild poliovirus in Cuba.</p>},
  author       = {Avalos Redón, I and Más Lago, P J and Sarmiento Pérez, L R and Palomera Puente, R and Muné Jiménez, M and Bello Corredor, M and Pérez Santos, L},
  issn         = {0375-0760},
  language     = {spa},
  number       = {2},
  pages        = {4--100},
  publisher    = {Editorial Ciencias Medicas},
  series       = {Revista cubana de medicina tropical},
  title        = {Caracterización intratípica de Poliovirus por la técnica de reacción en cadena de la polimerasa},
  url          = {http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0375-07601998000200004},
  volume       = {50},
  year         = {1998},
}