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Electrolyte handling in the isolated perfused rat kidney : demonstration of vasopressin V2-receptor-dependent calcium reabsorption

Bamberg, Krister ; William-Olsson, Lena ; Johansson, Ulrika ; Arner, Anders LU ; Hartleib-Geschwindner, Judith and Sällström, Johan (2020) In Upsala Journal of Medical Sciences 125(4). p.274-280
Abstract

Background: The most profound effect of vasopressin on the kidney is to increase water reabsorption through V2-receptor (V2R) stimulation, but there are also data suggesting effects on calcium transport. To address this issue, we have established an isolated perfused kidney model with accurate pressure control, to directly study the effects of V2R stimulation on kidney function, isolated from systemic effects. Methods: The role of V2R in renal calcium handling was studied in isolated rat kidneys using a new pressure control system that uses a calibration curve to compensate for the internal pressure drop up to the tip of the perfusion cannula. Results: Kidneys subjected to V2R... (More)

Background: The most profound effect of vasopressin on the kidney is to increase water reabsorption through V2-receptor (V2R) stimulation, but there are also data suggesting effects on calcium transport. To address this issue, we have established an isolated perfused kidney model with accurate pressure control, to directly study the effects of V2R stimulation on kidney function, isolated from systemic effects. Methods: The role of V2R in renal calcium handling was studied in isolated rat kidneys using a new pressure control system that uses a calibration curve to compensate for the internal pressure drop up to the tip of the perfusion cannula. Results: Kidneys subjected to V2R stimulation using desmopressin (DDAVP) displayed stable osmolality and calcium reabsorption throughout the experiment, whereas kidneys not administered DDAVP exhibited a simultaneous fall in urine osmolality and calcium reabsorption. Epithelial sodium channel (ENaC) inhibition using amiloride resulted in a marked increase in potassium reabsorption along with decreased sodium reabsorption. Conclusions: A stable isolated perfused kidney model with computer-controlled pressure regulation was developed, which retained key physiological functions. The preparation responds to pharmacological inhibition of ENaC channels and activation of V2R. Using the model, the dynamic effects of V2R stimulation on calcium handling and urine osmolality could be visualised. The study thereby provides evidence for a stimulatory role of V2R in renal calcium reabsorption.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
AVP, ENaC, kidney, vasopressin
in
Upsala Journal of Medical Sciences
volume
125
issue
4
pages
7 pages
publisher
Taylor & Francis
external identifiers
  • pmid:32812807
  • scopus:85089652778
ISSN
0300-9734
DOI
10.1080/03009734.2020.1804496
language
English
LU publication?
yes
id
99f99b31-fdef-4ded-80c0-4e1993f20628
date added to LUP
2021-01-15 16:12:25
date last changed
2024-04-03 23:14:43
@article{99f99b31-fdef-4ded-80c0-4e1993f20628,
  abstract     = {{<p>Background: The most profound effect of vasopressin on the kidney is to increase water reabsorption through V<sub>2</sub>-receptor (V<sub>2</sub>R) stimulation, but there are also data suggesting effects on calcium transport. To address this issue, we have established an isolated perfused kidney model with accurate pressure control, to directly study the effects of V<sub>2</sub>R stimulation on kidney function, isolated from systemic effects. Methods: The role of V<sub>2</sub>R in renal calcium handling was studied in isolated rat kidneys using a new pressure control system that uses a calibration curve to compensate for the internal pressure drop up to the tip of the perfusion cannula. Results: Kidneys subjected to V<sub>2</sub>R stimulation using desmopressin (DDAVP) displayed stable osmolality and calcium reabsorption throughout the experiment, whereas kidneys not administered DDAVP exhibited a simultaneous fall in urine osmolality and calcium reabsorption. Epithelial sodium channel (ENaC) inhibition using amiloride resulted in a marked increase in potassium reabsorption along with decreased sodium reabsorption. Conclusions: A stable isolated perfused kidney model with computer-controlled pressure regulation was developed, which retained key physiological functions. The preparation responds to pharmacological inhibition of ENaC channels and activation of V<sub>2</sub>R. Using the model, the dynamic effects of V<sub>2</sub>R stimulation on calcium handling and urine osmolality could be visualised. The study thereby provides evidence for a stimulatory role of V<sub>2</sub>R in renal calcium reabsorption.</p>}},
  author       = {{Bamberg, Krister and William-Olsson, Lena and Johansson, Ulrika and Arner, Anders and Hartleib-Geschwindner, Judith and Sällström, Johan}},
  issn         = {{0300-9734}},
  keywords     = {{AVP; ENaC; kidney; vasopressin}},
  language     = {{eng}},
  month        = {{10}},
  number       = {{4}},
  pages        = {{274--280}},
  publisher    = {{Taylor & Francis}},
  series       = {{Upsala Journal of Medical Sciences}},
  title        = {{Electrolyte handling in the isolated perfused rat kidney : demonstration of vasopressin V2-receptor-dependent calcium reabsorption}},
  url          = {{http://dx.doi.org/10.1080/03009734.2020.1804496}},
  doi          = {{10.1080/03009734.2020.1804496}},
  volume       = {{125}},
  year         = {{2020}},
}