Identification of protein biomarkers associated with congenital diaphragmatic hernia in human amniotic fluid
(2023) In Scientific Reports 13(1).- Abstract
Congenital diaphragmatic hernia (CDH) is a severe birth defect frequently associated with pulmonary hypoplasia, pulmonary hypertension, and heart failure. Since amniotic fluid comprises proteins of both fetal and maternal origin, its analysis could provide insights on mechanisms underlying CDH and provide biomarkers for early diagnosis, severity of pulmonary changes and treatment response. The study objective was to identify proteomic changes in amniotic fluid consistently associated with CDH. Amniotic fluid was obtained at term (37–39 weeks) from women with normal pregnancies (n = 5) or carrying fetuses with CDH (n = 5). After immuno-depletion of the highest abundance proteins, off-line fractionation and high-resolution tandem mass... (More)
Congenital diaphragmatic hernia (CDH) is a severe birth defect frequently associated with pulmonary hypoplasia, pulmonary hypertension, and heart failure. Since amniotic fluid comprises proteins of both fetal and maternal origin, its analysis could provide insights on mechanisms underlying CDH and provide biomarkers for early diagnosis, severity of pulmonary changes and treatment response. The study objective was to identify proteomic changes in amniotic fluid consistently associated with CDH. Amniotic fluid was obtained at term (37–39 weeks) from women with normal pregnancies (n = 5) or carrying fetuses with CDH (n = 5). After immuno-depletion of the highest abundance proteins, off-line fractionation and high-resolution tandem mass spectrometry were performed and quantitative differences between the proteomes of the groups were determined. Of 1036 proteins identified, 218 were differentially abundant. Bioinformatics analysis showed significant changes in GP6 signaling, in the MSP–RON signaling in macrophages pathway and in networks associated with cardiovascular system development and function, connective tissue disorders and dermatological conditions. Differences in selected proteins, namely pulmonary surfactant protein B, osteopontin, kallikrein 5 and galectin-3 were validated by orthogonal testing using ELISA in larger cohorts and showed statistically significant differences aiding in the diagnosis and prediction of CDH. The findings provide potential tools for clinical management of CDH.
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- author
- Bhutada, Sumit ; Tran-Lundmark, Karin LU ; Kramer, Benjamin ; Conner, Peter ; Lowry, Ashley M. ; Blackstone, Eugene ; Frenckner, Bjorn ; Mesas-Burgos, Carmen and Apte, Suneel S.
- organization
- publishing date
- 2023-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 13
- issue
- 1
- article number
- 15483
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:37726509
- scopus:85171436416
- ISSN
- 2045-2322
- DOI
- 10.1038/s41598-023-42576-2
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2023, Springer Nature Limited.
- id
- 9a72afe2-9c0f-4b62-b8a6-f0451922d1b3
- date added to LUP
- 2023-12-04 15:06:17
- date last changed
- 2024-04-17 11:59:33
@article{9a72afe2-9c0f-4b62-b8a6-f0451922d1b3,
abstract = {{<p>Congenital diaphragmatic hernia (CDH) is a severe birth defect frequently associated with pulmonary hypoplasia, pulmonary hypertension, and heart failure. Since amniotic fluid comprises proteins of both fetal and maternal origin, its analysis could provide insights on mechanisms underlying CDH and provide biomarkers for early diagnosis, severity of pulmonary changes and treatment response. The study objective was to identify proteomic changes in amniotic fluid consistently associated with CDH. Amniotic fluid was obtained at term (37–39 weeks) from women with normal pregnancies (n = 5) or carrying fetuses with CDH (n = 5). After immuno-depletion of the highest abundance proteins, off-line fractionation and high-resolution tandem mass spectrometry were performed and quantitative differences between the proteomes of the groups were determined. Of 1036 proteins identified, 218 were differentially abundant. Bioinformatics analysis showed significant changes in GP6 signaling, in the MSP–RON signaling in macrophages pathway and in networks associated with cardiovascular system development and function, connective tissue disorders and dermatological conditions. Differences in selected proteins, namely pulmonary surfactant protein B, osteopontin, kallikrein 5 and galectin-3 were validated by orthogonal testing using ELISA in larger cohorts and showed statistically significant differences aiding in the diagnosis and prediction of CDH. The findings provide potential tools for clinical management of CDH.</p>}},
author = {{Bhutada, Sumit and Tran-Lundmark, Karin and Kramer, Benjamin and Conner, Peter and Lowry, Ashley M. and Blackstone, Eugene and Frenckner, Bjorn and Mesas-Burgos, Carmen and Apte, Suneel S.}},
issn = {{2045-2322}},
language = {{eng}},
number = {{1}},
publisher = {{Nature Publishing Group}},
series = {{Scientific Reports}},
title = {{Identification of protein biomarkers associated with congenital diaphragmatic hernia in human amniotic fluid}},
url = {{http://dx.doi.org/10.1038/s41598-023-42576-2}},
doi = {{10.1038/s41598-023-42576-2}},
volume = {{13}},
year = {{2023}},
}