Anti-endotoxic and antibacterial effects of a dermal substitute coated with host defense peptides.
(2015) In Biomaterials 53. p.415-425- Abstract
- Biomaterials used during surgery and wound treatment are of increasing importance in modern medical care. In the present study we set out to evaluate the addition of thrombin-derived host defense peptides to human acellular dermis (hAD, i.e. epiflex(®)). Antimicrobial activity of the functionalized hAD was demonstrated using radial diffusion and viable count assays against Gram-negative Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Electron microscopy analyses showed that peptide-mediated bacterial killing led to reduced hAD degradation. Furthermore, peptide-functionalized hAD displayed endotoxin-binding activity in vitro, as evidenced by inhibition of NF-κB activation in human monocytic cells... (More)
- Biomaterials used during surgery and wound treatment are of increasing importance in modern medical care. In the present study we set out to evaluate the addition of thrombin-derived host defense peptides to human acellular dermis (hAD, i.e. epiflex(®)). Antimicrobial activity of the functionalized hAD was demonstrated using radial diffusion and viable count assays against Gram-negative Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Electron microscopy analyses showed that peptide-mediated bacterial killing led to reduced hAD degradation. Furthermore, peptide-functionalized hAD displayed endotoxin-binding activity in vitro, as evidenced by inhibition of NF-κB activation in human monocytic cells (THP-1 cells) and a reduction of pro-inflammatory cytokine production in whole blood in response to lipopolysaccharide stimulation. The dermal substitute retained its anti-endotoxic activity after washing, compatible with results showing that the hAD bound a significant amount of peptide. Furthermore, bacteria-induced contact activation was inhibited by peptide addition to the hAD. E. coli infected hAD, alone, or after treatment with the antiseptic substance polyhexamethylenebiguanide (PHMB), yielded NF-κB activation in THP-1 cells. The activation was abrogated by peptide addition. Thus, thrombin-derived HDPs should be of interest in the further development of new biomaterials with combined antimicrobial and anti-endotoxic functions for use in surgery and wound treatment. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5341307
- author
- Kasetty, Gopinath LU ; Kalle, Martina LU ; Mörgelin, Matthias LU ; Brune, Jan Claas LU and Schmidtchen, Artur LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Biomaterials
- volume
- 53
- pages
- 415 - 425
- publisher
- Elsevier
- external identifiers
-
- pmid:25890739
- wos:000353929800039
- scopus:84927938090
- ISSN
- 1878-5905
- DOI
- 10.1016/j.biomaterials.2015.02.111
- language
- English
- LU publication?
- yes
- id
- 9cc1215b-5d8c-40dc-8919-025bc4ce0aff (old id 5341307)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25890739?dopt=Abstract
- date added to LUP
- 2016-04-01 10:32:08
- date last changed
- 2022-08-20 03:42:11
@article{9cc1215b-5d8c-40dc-8919-025bc4ce0aff,
abstract = {{Biomaterials used during surgery and wound treatment are of increasing importance in modern medical care. In the present study we set out to evaluate the addition of thrombin-derived host defense peptides to human acellular dermis (hAD, i.e. epiflex(®)). Antimicrobial activity of the functionalized hAD was demonstrated using radial diffusion and viable count assays against Gram-negative Escherichia coli, Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus bacteria. Electron microscopy analyses showed that peptide-mediated bacterial killing led to reduced hAD degradation. Furthermore, peptide-functionalized hAD displayed endotoxin-binding activity in vitro, as evidenced by inhibition of NF-κB activation in human monocytic cells (THP-1 cells) and a reduction of pro-inflammatory cytokine production in whole blood in response to lipopolysaccharide stimulation. The dermal substitute retained its anti-endotoxic activity after washing, compatible with results showing that the hAD bound a significant amount of peptide. Furthermore, bacteria-induced contact activation was inhibited by peptide addition to the hAD. E. coli infected hAD, alone, or after treatment with the antiseptic substance polyhexamethylenebiguanide (PHMB), yielded NF-κB activation in THP-1 cells. The activation was abrogated by peptide addition. Thus, thrombin-derived HDPs should be of interest in the further development of new biomaterials with combined antimicrobial and anti-endotoxic functions for use in surgery and wound treatment.}},
author = {{Kasetty, Gopinath and Kalle, Martina and Mörgelin, Matthias and Brune, Jan Claas and Schmidtchen, Artur}},
issn = {{1878-5905}},
language = {{eng}},
pages = {{415--425}},
publisher = {{Elsevier}},
series = {{Biomaterials}},
title = {{Anti-endotoxic and antibacterial effects of a dermal substitute coated with host defense peptides.}},
url = {{https://lup.lub.lu.se/search/files/1925841/8227743}},
doi = {{10.1016/j.biomaterials.2015.02.111}},
volume = {{53}},
year = {{2015}},
}