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Ontogeny of vesicular monoamine transporter mRNAs VMAT1 and VMAT2 II. Expression in neural crest derivatives and their target sites in the rat

Hansson, Stefan R. LU orcid ; Mezey, Eva and Hoffman, Beth J. (1998) In Developmental Brain Research 110(1). p.159-174
Abstract

We used in situ hybridization histochemistry to study the expression of the two vesicular monoamine transporters (VMAT1 and VMAT2) during embryonic development in the rat. In the adult rat VMAT2 is present exclusively in neuronal tissues and VMAT1 is present in the adrenal medulla and in certain intestinal endocrine cells. We found that both transporter molecules are more widely expressed during development. We demonstrate a complete overlap of the two VMAT mRNAs in the sympathetic nervous system between E13 and E21 days. In addition, VMAT2 (and to some extent VMAT1) mRNA is expressed in ganglionic cells of the parasympathetic nervous system and in cranial ganglia (trigeminal, vestibular and spiral ganglia) between E12 and E21. The... (More)

We used in situ hybridization histochemistry to study the expression of the two vesicular monoamine transporters (VMAT1 and VMAT2) during embryonic development in the rat. In the adult rat VMAT2 is present exclusively in neuronal tissues and VMAT1 is present in the adrenal medulla and in certain intestinal endocrine cells. We found that both transporter molecules are more widely expressed during development. We demonstrate a complete overlap of the two VMAT mRNAs in the sympathetic nervous system between E13 and E21 days. In addition, VMAT2 (and to some extent VMAT1) mRNA is expressed in ganglionic cells of the parasympathetic nervous system and in cranial ganglia (trigeminal, vestibular and spiral ganglia) between E12 and E21. The sensory neurons of the dorsal root ganglia, which are also neural crest derivatives, express VMAT2 mRNA (E11-E21), exclusively. Both VMAT mRNAs are found in the developing GI system, but in different cells. VMAT1 mRNA was detected in organs of the endocrine system (pituitary gland, adrenal gland, testis, seminal vesicle), some connective tissue cells, and the thymus. We observed expression of both VMAT mRNAs in two separate cell groups in the placenta (E8-E10). Based on their distribution during development we suggest that monoamines, released in a controlled fashion, might affect migration and differentiation of neural crest derivatives.

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author
; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Biogenic amines, Dorsal root ganglia, Endocrine, Gastrointestinal tract, Heart, Placenta, Sympathetic ganglia
in
Developmental Brain Research
volume
110
issue
1
pages
16 pages
publisher
Elsevier
external identifiers
  • pmid:9733958
  • scopus:0032505028
ISSN
0165-3806
DOI
10.1016/S0165-3806(98)00103-5
language
English
LU publication?
no
id
9d3541e0-ac3d-4c3c-a579-7391694eba69
date added to LUP
2021-02-12 10:48:47
date last changed
2024-01-18 02:51:20
@article{9d3541e0-ac3d-4c3c-a579-7391694eba69,
  abstract     = {{<p>We used in situ hybridization histochemistry to study the expression of the two vesicular monoamine transporters (VMAT1 and VMAT2) during embryonic development in the rat. In the adult rat VMAT2 is present exclusively in neuronal tissues and VMAT1 is present in the adrenal medulla and in certain intestinal endocrine cells. We found that both transporter molecules are more widely expressed during development. We demonstrate a complete overlap of the two VMAT mRNAs in the sympathetic nervous system between E13 and E21 days. In addition, VMAT2 (and to some extent VMAT1) mRNA is expressed in ganglionic cells of the parasympathetic nervous system and in cranial ganglia (trigeminal, vestibular and spiral ganglia) between E12 and E21. The sensory neurons of the dorsal root ganglia, which are also neural crest derivatives, express VMAT2 mRNA (E11-E21), exclusively. Both VMAT mRNAs are found in the developing GI system, but in different cells. VMAT1 mRNA was detected in organs of the endocrine system (pituitary gland, adrenal gland, testis, seminal vesicle), some connective tissue cells, and the thymus. We observed expression of both VMAT mRNAs in two separate cell groups in the placenta (E8-E10). Based on their distribution during development we suggest that monoamines, released in a controlled fashion, might affect migration and differentiation of neural crest derivatives.</p>}},
  author       = {{Hansson, Stefan R. and Mezey, Eva and Hoffman, Beth J.}},
  issn         = {{0165-3806}},
  keywords     = {{Biogenic amines; Dorsal root ganglia; Endocrine; Gastrointestinal tract; Heart; Placenta; Sympathetic ganglia}},
  language     = {{eng}},
  month        = {{09}},
  number       = {{1}},
  pages        = {{159--174}},
  publisher    = {{Elsevier}},
  series       = {{Developmental Brain Research}},
  title        = {{Ontogeny of vesicular monoamine transporter mRNAs VMAT1 and VMAT2 II. Expression in neural crest derivatives and their target sites in the rat}},
  url          = {{http://dx.doi.org/10.1016/S0165-3806(98)00103-5}},
  doi          = {{10.1016/S0165-3806(98)00103-5}},
  volume       = {{110}},
  year         = {{1998}},
}