Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death
(2008) In Cell Biology International 32. p.66-74- Abstract
- The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin... (More)
- The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/744472
- author
- Holst, Martina LU ; Johansson, Veronica LU ; Alm, Kersti LU and Oredsson, Stina LU
- organization
- publishing date
- 2008
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Apoptosis, Spermine analogue, Cytochrome c, Human breast cancer, Bcl-2
- in
- Cell Biology International
- volume
- 32
- pages
- 66 - 74
- publisher
- Elsevier
- external identifiers
-
- wos:000260586200009
- scopus:39049171988
- pmid:17920946
- ISSN
- 1095-8355
- DOI
- 10.1016/j.cellbi.2007.08.011
- language
- English
- LU publication?
- yes
- id
- 9d500204-c53d-41df-9152-232b555aebea (old id 744472)
- date added to LUP
- 2016-04-01 11:44:31
- date last changed
- 2024-07-01 11:25:17
@article{9d500204-c53d-41df-9152-232b555aebea, abstract = {{The spermine analogue N(1),N(11)-diethylnorspermine (DENSPM) efficiently depletes the polyamine pools in the breast cancer cell line L56Br-C1 and induces apoptotic cell death via the mitochondrial pathway. In this study, we have over-expressed the anti-apoptotic protein Bcl-2 in L56Br-C1 cells and investigated the effect of DENSPM treatment. DENSPM-induced cell death was significantly reduced in Bcl-2 over-expressing cells. Bcl-2 over-expression reduced DENSPM-induced release of the pro-apoptotic proteins AIF, cytochrome c, and Smac/DIABLO from the mitochondria. Bcl-2 over-expression reduced the DENSPM-induced activation of caspase-3. Bcl-2 over-expression also prevented DENSPM-induced Bax cleavage and reduction of Bcl-X(L) and survivin levels. The DENSPM-induced activation of the polyamine catabolic enzyme spermidine/spermine N(1)-acetyltransferase was reduced by Bcl-2 over-expression, partly preventing polyamine depletion. Thus, Bcl-2 over-expression prevented a number of DENSPM-induced apoptotic effects}}, author = {{Holst, Martina and Johansson, Veronica and Alm, Kersti and Oredsson, Stina}}, issn = {{1095-8355}}, keywords = {{Apoptosis; Spermine analogue; Cytochrome c; Human breast cancer; Bcl-2}}, language = {{eng}}, pages = {{66--74}}, publisher = {{Elsevier}}, series = {{Cell Biology International}}, title = {{Novel anti-apoptotic effect of Bcl-2: Prevention of polyamine depletion-induced cell death}}, url = {{http://dx.doi.org/10.1016/j.cellbi.2007.08.011}}, doi = {{10.1016/j.cellbi.2007.08.011}}, volume = {{32}}, year = {{2008}}, }