Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap
(2011) In Preparative Biochemistry & Biotechnology 41(2). p.166-174- Abstract
- A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We... (More)
- A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1918859
- author
- Gronlund, Hugo ; Moen, Birgitte ; Hoorfar, Jeffrey ; Rådström, Peter LU ; Malorny, Burkhard and Rudi, Knut
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- multiplex, Salmonella, SNP
- in
- Preparative Biochemistry & Biotechnology
- volume
- 41
- issue
- 2
- pages
- 166 - 174
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000288960600005
- scopus:79953229607
- pmid:21442552
- ISSN
- 1532-2297
- DOI
- 10.1080/10826068.2011.547366
- language
- English
- LU publication?
- yes
- id
- 9e3a534a-3150-4284-bfac-6d9c90eb047a (old id 1918859)
- date added to LUP
- 2016-04-01 13:10:56
- date last changed
- 2022-01-27 17:50:56
@article{9e3a534a-3150-4284-bfac-6d9c90eb047a,
abstract = {{A major challenge with single-nucleotide polymorphism (SNP) fingerprinting of bacteria and higher organisms is the combination of genome-wide screenings with the potential of multiplexing and accurate SNP detection. Single-nucleotide extension by the minisequencing principle represents a technology that both is highly accurate and enables multiplexing. A current bottleneck for direct genome analyses by minisequencing, however, is the sensitivity, since minisequencing relies on linear signal amplification. Here, we present SNPtrap, which is a novel approach that combines the specificity and possibility of multiplexing by minisequencing with the sensitivity obtained by logarithmic signal amplification by polymerase chain reaction (PCR). We show a SNPtrap proof of principle in a model system for two polymorphic SNP sites in the Salmonella tetrathionate reductase gene (ttrC).}},
author = {{Gronlund, Hugo and Moen, Birgitte and Hoorfar, Jeffrey and Rådström, Peter and Malorny, Burkhard and Rudi, Knut}},
issn = {{1532-2297}},
keywords = {{multiplex; Salmonella; SNP}},
language = {{eng}},
number = {{2}},
pages = {{166--174}},
publisher = {{Taylor & Francis}},
series = {{Preparative Biochemistry & Biotechnology}},
title = {{Direct Detection Of Single-Nucleotide Polymorphisms In Bacterial DNA By SNPtrap}},
url = {{http://dx.doi.org/10.1080/10826068.2011.547366}},
doi = {{10.1080/10826068.2011.547366}},
volume = {{41}},
year = {{2011}},
}