Increased Neointimal Thickening in Dystrophin-Deficient mdx Mice.
(2012) In PLoS ONE 7(1).- Abstract
- BACKGROUND:
The dystrophin gene, which is mutated in Duchenne muscular dystrophy (DMD), encodes a large cytoskeletal protein present in muscle fibers. While dystrophin in skeletal muscle has been extensively studied, the function of dystrophin in vascular smooth muscle is less clear. Here, we have analyzed the role of dystrophin in injury-induced arterial neointima formation.
METHODOLOGY/PRINCIPAL FINDINGS:
We detected a down-regulation of dystrophin, dystroglycan and β-sarcoglycan mRNA expression when vascular smooth muscle cells de-differentiate in vitro. To further mimic development of intimal lesions, we performed a collar-induced injury of the carotid artery in the mdx mouse, a model for DMD. As compared... (More) - BACKGROUND:
The dystrophin gene, which is mutated in Duchenne muscular dystrophy (DMD), encodes a large cytoskeletal protein present in muscle fibers. While dystrophin in skeletal muscle has been extensively studied, the function of dystrophin in vascular smooth muscle is less clear. Here, we have analyzed the role of dystrophin in injury-induced arterial neointima formation.
METHODOLOGY/PRINCIPAL FINDINGS:
We detected a down-regulation of dystrophin, dystroglycan and β-sarcoglycan mRNA expression when vascular smooth muscle cells de-differentiate in vitro. To further mimic development of intimal lesions, we performed a collar-induced injury of the carotid artery in the mdx mouse, a model for DMD. As compared with control mice, mdx mice develop larger lesions with increased numbers of proliferating cells. In vitro experiments demonstrate increased migration of vascular smooth muscle cells from mdx mice whereas the rate of proliferation was similar in cells isolated from wild-type and mdx mice.
CONCLUSIONS/SIGNIFICANCE:
These results show that dystrophin deficiency stimulates neointima formation and suggest that expression of dystrophin in vascular smooth muscle cells may protect the artery wall against injury-induced intimal thickening. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2336489
- author
- Rauch, Uwe LU ; Shami, Annelie LU ; Zhang, Feng LU ; Carmignac, Virginie LU ; Durbeej-Hjalt, Madeleine LU and Hultgårdh, Anna LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 7
- issue
- 1
- article number
- e29904
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- wos:000301070200057
- pmid:22238670
- scopus:84855388573
- pmid:22238670
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0029904
- language
- English
- LU publication?
- yes
- id
- 9f1a61d3-d5ba-48fd-8ded-6619ed1d1dd8 (old id 2336489)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/22238670?dopt=Abstract
- date added to LUP
- 2016-04-01 13:50:44
- date last changed
- 2023-01-04 01:12:58
@article{9f1a61d3-d5ba-48fd-8ded-6619ed1d1dd8, abstract = {{BACKGROUND:<br/><br> The dystrophin gene, which is mutated in Duchenne muscular dystrophy (DMD), encodes a large cytoskeletal protein present in muscle fibers. While dystrophin in skeletal muscle has been extensively studied, the function of dystrophin in vascular smooth muscle is less clear. Here, we have analyzed the role of dystrophin in injury-induced arterial neointima formation.<br/><br> METHODOLOGY/PRINCIPAL FINDINGS:<br/><br> We detected a down-regulation of dystrophin, dystroglycan and β-sarcoglycan mRNA expression when vascular smooth muscle cells de-differentiate in vitro. To further mimic development of intimal lesions, we performed a collar-induced injury of the carotid artery in the mdx mouse, a model for DMD. As compared with control mice, mdx mice develop larger lesions with increased numbers of proliferating cells. In vitro experiments demonstrate increased migration of vascular smooth muscle cells from mdx mice whereas the rate of proliferation was similar in cells isolated from wild-type and mdx mice.<br/><br> CONCLUSIONS/SIGNIFICANCE:<br/><br> These results show that dystrophin deficiency stimulates neointima formation and suggest that expression of dystrophin in vascular smooth muscle cells may protect the artery wall against injury-induced intimal thickening.}}, author = {{Rauch, Uwe and Shami, Annelie and Zhang, Feng and Carmignac, Virginie and Durbeej-Hjalt, Madeleine and Hultgårdh, Anna}}, issn = {{1932-6203}}, language = {{eng}}, number = {{1}}, publisher = {{Public Library of Science (PLoS)}}, series = {{PLoS ONE}}, title = {{Increased Neointimal Thickening in Dystrophin-Deficient mdx Mice.}}, url = {{https://lup.lub.lu.se/search/files/3622885/2369413.pdf}}, doi = {{10.1371/journal.pone.0029904}}, volume = {{7}}, year = {{2012}}, }