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Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant

Cunha, André B. ; Schuelke, Christin ; Mesri, Alireza ; Ruud, Simen K. ; Aizenshtadt, Aleksandra ; Ferrari, Giorgio ; Heiskanen, Arto ; Asif, Afia ; Keller, Stephan S. and Ramos-Moreno, Tania LU orcid , et al. (2024) In Sensors 24(2).
Abstract

Implantable cell replacement therapies promise to completely restore the function of neural structures, possibly changing how we currently perceive the onset of neurodegenerative diseases. One of the major clinical hurdles for the routine implementation of stem cell therapies is poor cell retention and survival, demanding the need to better understand these mechanisms while providing precise and scalable approaches to monitor these cell-based therapies in both pre-clinical and clinical scenarios. This poses significant multidisciplinary challenges regarding planning, defining the methodology and requirements, prototyping and different stages of testing. Aiming toward an optogenetic neural stem cell implant controlled by a smart wireless... (More)

Implantable cell replacement therapies promise to completely restore the function of neural structures, possibly changing how we currently perceive the onset of neurodegenerative diseases. One of the major clinical hurdles for the routine implementation of stem cell therapies is poor cell retention and survival, demanding the need to better understand these mechanisms while providing precise and scalable approaches to monitor these cell-based therapies in both pre-clinical and clinical scenarios. This poses significant multidisciplinary challenges regarding planning, defining the methodology and requirements, prototyping and different stages of testing. Aiming toward an optogenetic neural stem cell implant controlled by a smart wireless electronic frontend, we show how an iterative development methodology coupled with a modular design philosophy can mitigate some of these challenges. In this study, we present a miniaturized, wireless-controlled, modular multisensor platform with fully interfaced electronics featuring three different modules: an impedance analyzer, a potentiostat and an optical stimulator. We show the application of the platform for electrical impedance spectroscopy-based cell monitoring, optical stimulation to induce dopamine release from optogenetically modified neurons and a potentiostat for cyclic voltammetry and amperometric detection of dopamine release. The multisensor platform is designed to be used as an opto-electric headstage for future in vivo animal experiments.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
amperometry, brain implant, cyclic voltammetry, dopamine, electrochemistry, impedance, neural stem cells, optogenetics, Parkinson’s disease, PSoC
in
Sensors
volume
24
issue
2
article number
575
publisher
MDPI AG
external identifiers
  • pmid:38257668
  • scopus:85183266026
ISSN
1424-8220
DOI
10.3390/s24020575
language
English
LU publication?
yes
id
9f30473f-e635-46ba-9961-1483fa454508
date added to LUP
2024-02-21 13:59:14
date last changed
2024-04-21 09:41:39
@article{9f30473f-e635-46ba-9961-1483fa454508,
  abstract     = {{<p>Implantable cell replacement therapies promise to completely restore the function of neural structures, possibly changing how we currently perceive the onset of neurodegenerative diseases. One of the major clinical hurdles for the routine implementation of stem cell therapies is poor cell retention and survival, demanding the need to better understand these mechanisms while providing precise and scalable approaches to monitor these cell-based therapies in both pre-clinical and clinical scenarios. This poses significant multidisciplinary challenges regarding planning, defining the methodology and requirements, prototyping and different stages of testing. Aiming toward an optogenetic neural stem cell implant controlled by a smart wireless electronic frontend, we show how an iterative development methodology coupled with a modular design philosophy can mitigate some of these challenges. In this study, we present a miniaturized, wireless-controlled, modular multisensor platform with fully interfaced electronics featuring three different modules: an impedance analyzer, a potentiostat and an optical stimulator. We show the application of the platform for electrical impedance spectroscopy-based cell monitoring, optical stimulation to induce dopamine release from optogenetically modified neurons and a potentiostat for cyclic voltammetry and amperometric detection of dopamine release. The multisensor platform is designed to be used as an opto-electric headstage for future in vivo animal experiments.</p>}},
  author       = {{Cunha, André B. and Schuelke, Christin and Mesri, Alireza and Ruud, Simen K. and Aizenshtadt, Aleksandra and Ferrari, Giorgio and Heiskanen, Arto and Asif, Afia and Keller, Stephan S. and Ramos-Moreno, Tania and Kalvøy, Håvard and Martínez-Serrano, Alberto and Krauss, Stefan and Emnéus, Jenny and Sampietro, Marco and Martinsen, Ørjan G.}},
  issn         = {{1424-8220}},
  keywords     = {{amperometry; brain implant; cyclic voltammetry; dopamine; electrochemistry; impedance; neural stem cells; optogenetics; Parkinson’s disease; PSoC}},
  language     = {{eng}},
  number       = {{2}},
  publisher    = {{MDPI AG}},
  series       = {{Sensors}},
  title        = {{Development of a Smart Wireless Multisensor Platform for an Optogenetic Brain Implant}},
  url          = {{http://dx.doi.org/10.3390/s24020575}},
  doi          = {{10.3390/s24020575}},
  volume       = {{24}},
  year         = {{2024}},
}