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Acoustofluidic harvesting of microalgae on a single chip

Park, Jee Woong; Kim, Soo Hyeon; Ito, Takuro; Fujii, Teruo; Kim, So Youn; Laurell, Thomas LU ; Lee, Sang Wook LU and Goda, Keisuke (2016) In Biomicrofluidics 10(3).
Abstract

We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76,... (More)

We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomicrofluidics
volume
10
issue
3
publisher
American Institute of Physics
external identifiers
  • scopus:84975452243
  • wos:000379162400033
ISSN
1932-1058
DOI
10.1063/1.4954744
language
English
LU publication?
yes
id
a0714132-8b94-43a0-bf4b-e40d6adf0a7c
date added to LUP
2017-02-02 13:53:17
date last changed
2017-09-18 11:35:29
@article{a0714132-8b94-43a0-bf4b-e40d6adf0a7c,
  abstract     = {<p>We present an on-chip acoustofluidic platform for harvesting a target microalgal species from a heterogeneous population of cells and particles based on their size, density, and compressibility in a rapid, non-invasive, energy-efficient, continuously running, and automated manner. For our proof-of-principle demonstration, we use Euglena gracilis as a target species. Specifically, we show the simultaneous separation and enrichment of E. gracilis from a mixed population of E. gracilis in pond water (consisting of other microalgae and various kinds of particles as contaminants) on a single acoustofluidic chip with a recovery ratio of 92.6%, a target separation ratio of 90.1%, a concentration factor of 3.43, an enrichment factor of 12.76, and a cell viability rate of 98.3% at a high volume rate of 500 μl/min. Our results indicate that the on-chip acoustofluidic platform is an effective tool for harvesting target microalgae from mixed populations of microalgae and other contaminants.</p>},
  articleno    = {034119},
  author       = {Park, Jee Woong and Kim, Soo Hyeon and Ito, Takuro and Fujii, Teruo and Kim, So Youn and Laurell, Thomas and Lee, Sang Wook and Goda, Keisuke},
  issn         = {1932-1058},
  language     = {eng},
  month        = {05},
  number       = {3},
  publisher    = {American Institute of Physics},
  series       = {Biomicrofluidics},
  title        = {Acoustofluidic harvesting of microalgae on a single chip},
  url          = {http://dx.doi.org/10.1063/1.4954744},
  volume       = {10},
  year         = {2016},
}