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Impaired glucose transport in inguinal adipocytes after short-term high-sucrose feeding in mice

Fryklund, Claes LU ; Borg, Madelene ; Svensson, Tobias ; Schumacher, Sara ; Negoita, Florentina LU ; Morén, Björn LU and Stenkula, Karin G. LU (2020) In Journal of Nutritional Biochemistry 78.
Abstract

Diets enriched in sucrose severely impair metabolic regulation and are associated with obesity, insulin resistance and glucose intolerance. In the current study, we investigated the effect of 4 weeks high-sucrose diet (HSD) feeding in C57BL6/J mice, with specific focus on adipocyte function. Mice fed HSD had slightly increased adipose tissue mass but displayed similar hepatic triglycerides, glucose and insulin levels, and glucose clearance capacity as chow-fed mice. Interestingly, we found adipose depot-specific differences, where both the non- and insulin-stimulated glucose transports were markedly impaired in primary adipocytes isolated from the inguinal fat depot from HSD-fed mice. This was accompanied by decreased protein levels of... (More)

Diets enriched in sucrose severely impair metabolic regulation and are associated with obesity, insulin resistance and glucose intolerance. In the current study, we investigated the effect of 4 weeks high-sucrose diet (HSD) feeding in C57BL6/J mice, with specific focus on adipocyte function. Mice fed HSD had slightly increased adipose tissue mass but displayed similar hepatic triglycerides, glucose and insulin levels, and glucose clearance capacity as chow-fed mice. Interestingly, we found adipose depot-specific differences, where both the non- and insulin-stimulated glucose transports were markedly impaired in primary adipocytes isolated from the inguinal fat depot from HSD-fed mice. This was accompanied by decreased protein levels of both GLUT4 and AS160. A similar but much less pronounced trend was observed in the retroperitoneal depot. In contrast, both GLUT4 expression and insulin-stimulated glucose uptake were preserved in adipocytes isolated from epididymal adipose tissue with HSD. Further, we found a slight shift in cell size distribution towards larger cells with HSD and a significant decrease of ACC and PGC-1α expression in the inguinal adipose tissue depot. Moreover, fructose alone was sufficient to decrease GLUT4 expression in cultured, mature adipocytes. Altogether, we demonstrate that short-term HSD feeding has deleterious impact on insulin response and glucose transport in the inguinal adipose tissue depot, specifically. These changes occur before the onset of systemic glucose dysmetabolism and therefore could provide a mechanistic link to overall impaired energy metabolism reported after prolonged HSD feeding, alone or in combination with HFD.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Adipocytes, Adipose tissue, Fructose, Glucose transport, GLUT4, High-sucrose diet
in
Journal of Nutritional Biochemistry
volume
78
article number
108338
publisher
Elsevier
external identifiers
  • scopus:85078284051
  • pmid:32004930
ISSN
0955-2863
DOI
10.1016/j.jnutbio.2019.108338
language
English
LU publication?
yes
id
a0c87550-39c5-4af3-8b78-4e06f58e404f
date added to LUP
2020-02-04 11:10:32
date last changed
2020-02-12 10:20:32
@article{a0c87550-39c5-4af3-8b78-4e06f58e404f,
  abstract     = {<p>Diets enriched in sucrose severely impair metabolic regulation and are associated with obesity, insulin resistance and glucose intolerance. In the current study, we investigated the effect of 4 weeks high-sucrose diet (HSD) feeding in C57BL6/J mice, with specific focus on adipocyte function. Mice fed HSD had slightly increased adipose tissue mass but displayed similar hepatic triglycerides, glucose and insulin levels, and glucose clearance capacity as chow-fed mice. Interestingly, we found adipose depot-specific differences, where both the non- and insulin-stimulated glucose transports were markedly impaired in primary adipocytes isolated from the inguinal fat depot from HSD-fed mice. This was accompanied by decreased protein levels of both GLUT4 and AS160. A similar but much less pronounced trend was observed in the retroperitoneal depot. In contrast, both GLUT4 expression and insulin-stimulated glucose uptake were preserved in adipocytes isolated from epididymal adipose tissue with HSD. Further, we found a slight shift in cell size distribution towards larger cells with HSD and a significant decrease of ACC and PGC-1α expression in the inguinal adipose tissue depot. Moreover, fructose alone was sufficient to decrease GLUT4 expression in cultured, mature adipocytes. Altogether, we demonstrate that short-term HSD feeding has deleterious impact on insulin response and glucose transport in the inguinal adipose tissue depot, specifically. These changes occur before the onset of systemic glucose dysmetabolism and therefore could provide a mechanistic link to overall impaired energy metabolism reported after prolonged HSD feeding, alone or in combination with HFD.</p>},
  author       = {Fryklund, Claes and Borg, Madelene and Svensson, Tobias and Schumacher, Sara and Negoita, Florentina and Morén, Björn and Stenkula, Karin G.},
  issn         = {0955-2863},
  language     = {eng},
  publisher    = {Elsevier},
  series       = {Journal of Nutritional Biochemistry},
  title        = {Impaired glucose transport in inguinal adipocytes after short-term high-sucrose feeding in mice},
  url          = {http://dx.doi.org/10.1016/j.jnutbio.2019.108338},
  doi          = {10.1016/j.jnutbio.2019.108338},
  volume       = {78},
  year         = {2020},
}