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Coimmobilization of substrate and biocatalyst : A method for bioconversion of poorly soluble substances in water milieu

Kaul, Rajni LU ; Adlercreutz, Patrick LU orcid and Mattiasson, Bo LU (1986) In Biotechnology and Bioengineering 28(9). p.1432-1437
Abstract

Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.

Please use this url to cite or link to this publication:
author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology and Bioengineering
volume
28
issue
9
pages
6 pages
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:0022787937
ISSN
0006-3592
DOI
10.1002/bit.260280917
language
English
LU publication?
yes
id
a13668f8-be86-4f90-9f45-3d1aecfa652a
date added to LUP
2019-06-22 19:02:18
date last changed
2021-01-03 07:59:00
@article{a13668f8-be86-4f90-9f45-3d1aecfa652a,
  abstract     = {{<p>Coimmobilization of biocatalyst and substrate was studied as a method to increase the conversion rate in systems with substrates of extremely low solubility in water. The system studied was the conversion of hydrocortisone to prednisolone by Arthrobacter simplex. As a matrix for coimmobilization, alginate turned out to be superior to agar and agarose. After the reaction was complete, the beads were solubilized, andthe cells recovered for reuse, by centrifugation, whereas the prednisolone was extracted from the supernatant.</p>}},
  author       = {{Kaul, Rajni and Adlercreutz, Patrick and Mattiasson, Bo}},
  issn         = {{0006-3592}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{9}},
  pages        = {{1432--1437}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Biotechnology and Bioengineering}},
  title        = {{Coimmobilization of substrate and biocatalyst : A method for bioconversion of poorly soluble substances in water milieu}},
  url          = {{http://dx.doi.org/10.1002/bit.260280917}},
  doi          = {{10.1002/bit.260280917}},
  volume       = {{28}},
  year         = {{1986}},
}