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Mutations in HPV16 L1 hypervariable surface-exposed loops affect L2 binding and DNA encapsidation.

Faust, Helena LU and Dillner, Joakim LU (2013) In Journal of General Virology 94(May,8). p.1841-1849
Abstract
Prophylactic vaccines against human papillomavirus (HPV) based on Virus-Like Particles (VLPs) induce type-specific neutralizing antibodies against a small number of hypervariable residues positioned in surface-exposed loops of the major capsid protein L1. To investigate the importance of these residues for neutralization, cross-neutralization, L2 incorporation and genome encapsidation, ten surface-exposed amino acid residues in 4 hypervariable loops of L1 were mutated. VLPs containing mutated or wild type L1, with or without wild type L2, were produced in 293TT cells using pseudovirion expression vectors. The mutations reduced the ability to induce neutralizing antibodies and to incorporate the L2 protein in the capsid. Ability to induce... (More)
Prophylactic vaccines against human papillomavirus (HPV) based on Virus-Like Particles (VLPs) induce type-specific neutralizing antibodies against a small number of hypervariable residues positioned in surface-exposed loops of the major capsid protein L1. To investigate the importance of these residues for neutralization, cross-neutralization, L2 incorporation and genome encapsidation, ten surface-exposed amino acid residues in 4 hypervariable loops of L1 were mutated. VLPs containing mutated or wild type L1, with or without wild type L2, were produced in 293TT cells using pseudovirion expression vectors. The mutations reduced the ability to induce neutralizing antibodies and to incorporate the L2 protein in the capsid. Ability to induce cross-neutralizing antibodies and to encapsidate pseudogenomes were completely abrogated. In summary, the surface-exposed L1 loops are important for the function of the HPV particle. (Less)
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author
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organization
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type
Contribution to journal
publication status
published
subject
in
Journal of General Virology
volume
94
issue
May,8
pages
1841 - 1849
publisher
Microbiology Society
external identifiers
  • wos:000322850200015
  • pmid:23658208
  • scopus:84880156023
  • pmid:23658208
ISSN
1465-2099
DOI
10.1099/vir.0.054205-0
language
English
LU publication?
yes
id
a1869edd-d89e-4ee3-ab05-b105e60f570f (old id 3804728)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23658208?dopt=Abstract
date added to LUP
2016-04-01 11:17:16
date last changed
2022-02-18 01:34:04
@article{a1869edd-d89e-4ee3-ab05-b105e60f570f,
  abstract     = {{Prophylactic vaccines against human papillomavirus (HPV) based on Virus-Like Particles (VLPs) induce type-specific neutralizing antibodies against a small number of hypervariable residues positioned in surface-exposed loops of the major capsid protein L1. To investigate the importance of these residues for neutralization, cross-neutralization, L2 incorporation and genome encapsidation, ten surface-exposed amino acid residues in 4 hypervariable loops of L1 were mutated. VLPs containing mutated or wild type L1, with or without wild type L2, were produced in 293TT cells using pseudovirion expression vectors. The mutations reduced the ability to induce neutralizing antibodies and to incorporate the L2 protein in the capsid. Ability to induce cross-neutralizing antibodies and to encapsidate pseudogenomes were completely abrogated. In summary, the surface-exposed L1 loops are important for the function of the HPV particle.}},
  author       = {{Faust, Helena and Dillner, Joakim}},
  issn         = {{1465-2099}},
  language     = {{eng}},
  number       = {{May,8}},
  pages        = {{1841--1849}},
  publisher    = {{Microbiology Society}},
  series       = {{Journal of General Virology}},
  title        = {{Mutations in HPV16 L1 hypervariable surface-exposed loops affect L2 binding and DNA encapsidation.}},
  url          = {{http://dx.doi.org/10.1099/vir.0.054205-0}},
  doi          = {{10.1099/vir.0.054205-0}},
  volume       = {{94}},
  year         = {{2013}},
}