Decomplexing biofluids using microchip based acoustophoresis
Augustsson, Per LU ; Persson, Jonas LU ; Ekström, Simon LU ; Ohlin, Mats LU
and Laurell, Thomas
LU
(2009)
In Lab on a Chip
9(6).
p.810-818
- Abstract
- Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart... (More)
- Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10(-6) of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1265242
- author
- Augustsson, Per
LU
; Persson, Jonas
LU
; Ekström, Simon
LU
; Ohlin, Mats
LU
and Laurell, Thomas
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Lab on a Chip
- volume
- 9
- issue
- 6
- pages
- 810 - 818
- publisher
- Royal Society of Chemistry
- external identifiers
-
- wos:000263847000009
- pmid:19255663
- scopus:61849089803
- ISSN
- 1473-0189
- DOI
- 10.1039/B811027A
- language
- English
- LU publication?
- yes
- id
- a2a63fe1-5922-4ca7-89d9-7281b2d6f44d (old id 1265242)
- date added to LUP
- 2016-04-01 11:51:20
- date last changed
- 2022-04-28 21:01:13
@article{a2a63fe1-5922-4ca7-89d9-7281b2d6f44d,
abstract = {{Highly efficient washing and extraction of microbeads to decomplex analytes ranging from small peptides to large viruses was realised in a microscaled continuous flow format. The bead washing principle reported herein is based on acoustophoresis, i.e. the primary acoustic radiation force in an ultrasonic standing wave and laminar flow properties are utilised to translate bioanalytes trapped on functionalised microbeads from one carrier fluid to another. The carry-over of non-specific material ranges from 1 to 50 ppm relative to input levels depending on application, making acoustophoresis suitable for extraction of rare species from complex environments. Selective extraction of a phosphopeptide relative to its unphosphorylated counterpart is demonstrated using metal oxide affinity capture (MOAC) beads and MALDI-TOF MS readout. Acoustophoresis of microbeads activated with specific binders could be used to capture phage viral particles. The efficiency of the acoustophoretic washing principle was demonstrated by an unspecific phage cross contamination level of only 10(-6) of that in the input bead/phage mixture. The continuous flow format makes acoustophoretic washing flexible regarding sample volume and also allows for easy integration into a sequence of particle handling and analytical unit operations.}},
author = {{Augustsson, Per and Persson, Jonas and Ekström, Simon and Ohlin, Mats and Laurell, Thomas}},
issn = {{1473-0189}},
language = {{eng}},
number = {{6}},
pages = {{810--818}},
publisher = {{Royal Society of Chemistry}},
series = {{Lab on a Chip}},
title = {{Decomplexing biofluids using microchip based acoustophoresis}},
url = {{http://dx.doi.org/10.1039/B811027A}},
doi = {{10.1039/B811027A}},
volume = {{9}},
year = {{2009}},
}