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Complex formation between chymotrypsin and ethyl cellulose as a means to solubilize the enzyme in active form in toluene

Otamiri, Marina ; Adlercreutz, Patrick LU and Mattiasson, Bo LU (1992) In Biocatalysis and Biotransformation 6(4). p.291-305
Abstract

Chymotryspin and ethyl cellulose were mixed in an aqueous phosphate buffer solution and freeze dried. Due to complex formation between the substances it was possible to dissolve or at least finely disperse these preparations in toluene. The chymotrypsin-ethyl cellulose complexes were characterized by light scattering measurements. Complexes were also formed by mixing enzyme powder in toluene containing ethyl cellulose and buffer but this was a slow process. Experiments with radioactively labelled bovine serum albumin showed that this protein was also solubilized in toluene in the presence of ethyl cellulose and buffer salts. Chymotrypsin complexes were used to catalyze the esterification of N-acetyl-L-phenylalanine with ethanol in... (More)

Chymotryspin and ethyl cellulose were mixed in an aqueous phosphate buffer solution and freeze dried. Due to complex formation between the substances it was possible to dissolve or at least finely disperse these preparations in toluene. The chymotrypsin-ethyl cellulose complexes were characterized by light scattering measurements. Complexes were also formed by mixing enzyme powder in toluene containing ethyl cellulose and buffer but this was a slow process. Experiments with radioactively labelled bovine serum albumin showed that this protein was also solubilized in toluene in the presence of ethyl cellulose and buffer salts. Chymotrypsin complexes were used to catalyze the esterification of N-acetyl-L-phenylalanine with ethanol in toluene. The presence of buffer salts greatly increased the initial reaction rate of the esterification reaction. The complexes were consideraly more active and stable than enzyme powder in toluene.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bioorganic synthesis, Ethyl cellulose, Light scattering, Modified chymotrypsin, Polystyrene
in
Biocatalysis and Biotransformation
volume
6
issue
4
pages
15 pages
publisher
Taylor & Francis
external identifiers
  • scopus:0000714375
ISSN
1024-2422
DOI
10.3109/10242429209065249
language
English
LU publication?
yes
id
a2f667c2-3af7-44d8-93b3-4693013bc7fb
date added to LUP
2019-06-22 18:41:48
date last changed
2020-01-13 02:05:05
@article{a2f667c2-3af7-44d8-93b3-4693013bc7fb,
  abstract     = {<p>Chymotryspin and ethyl cellulose were mixed in an aqueous phosphate buffer solution and freeze dried. Due to complex formation between the substances it was possible to dissolve or at least finely disperse these preparations in toluene. The chymotrypsin-ethyl cellulose complexes were characterized by light scattering measurements. Complexes were also formed by mixing enzyme powder in toluene containing ethyl cellulose and buffer but this was a slow process. Experiments with radioactively labelled bovine serum albumin showed that this protein was also solubilized in toluene in the presence of ethyl cellulose and buffer salts. Chymotrypsin complexes were used to catalyze the esterification of N-acetyl-L-phenylalanine with ethanol in toluene. The presence of buffer salts greatly increased the initial reaction rate of the esterification reaction. The complexes were consideraly more active and stable than enzyme powder in toluene.</p>},
  author       = {Otamiri, Marina and Adlercreutz, Patrick and Mattiasson, Bo},
  issn         = {1024-2422},
  language     = {eng},
  month        = {01},
  number       = {4},
  pages        = {291--305},
  publisher    = {Taylor & Francis},
  series       = {Biocatalysis and Biotransformation},
  title        = {Complex formation between chymotrypsin and ethyl cellulose as a means to solubilize the enzyme in active form in toluene},
  url          = {http://dx.doi.org/10.3109/10242429209065249},
  doi          = {10.3109/10242429209065249},
  volume       = {6},
  year         = {1992},
}