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Immune profile in blood following non-convulsive epileptic seizures in rats

Avdic, Una LU ; Ahl, Matilda LU ; Öberg, Maria and Ekdahl, Christine T. LU (2019) In Frontiers in Neurology 10(JUL).
Abstract

Non-convulsive status epilepticus (NCSE) is a prolonged epileptic seizure with subtle symptoms that may delay clinical diagnosis. Emerging experimental evidence shows brain pathology and epilepsy development following NCSE. New diagnostic/prognostic tools are therefore needed for earlier and better stratification of treatment. Here we examined whether NCSE initiates a peripheral immune response in blood serum from rats that experienced electrically-induced NCSE. ELISA analysis showed an acute transient increase in serum protein levels including interleukin-6 6 h post-NCSE, similar to the immune reaction in the brain. At 4 weeks post-NCSE, when 75% of rats subjected to NCSE had also developed spontaneous seizures, several immune proteins... (More)

Non-convulsive status epilepticus (NCSE) is a prolonged epileptic seizure with subtle symptoms that may delay clinical diagnosis. Emerging experimental evidence shows brain pathology and epilepsy development following NCSE. New diagnostic/prognostic tools are therefore needed for earlier and better stratification of treatment. Here we examined whether NCSE initiates a peripheral immune response in blood serum from rats that experienced electrically-induced NCSE. ELISA analysis showed an acute transient increase in serum protein levels including interleukin-6 6 h post-NCSE, similar to the immune reaction in the brain. At 4 weeks post-NCSE, when 75% of rats subjected to NCSE had also developed spontaneous seizures, several immune proteins were altered. In particular, markers associated with microglia, macrophages and antigen presenting cells, such as CD68, MHCII, and galectin-3, were increased and the T-cell marker CD4 was decreased in serum compared to both non-stimulated controls and NCSE rats without spontaneous seizures, without correlation to interictal epileptiform activity. Analyses of serum following intracerebral injection of lipopolysaccharide (LPS) showed an acute increase in interleukin-6, but at 4 weeks unaltered levels of MHCII and galectin-3, an increase in CD8 and CD11b and a decrease in CD68. None of the increased serum protein levels after NCSE or LPS could be confirmed in spleen tissue. Our data identifies the possibility to detect peripheral changes in serum protein levels following NCSE, which may be related to the development of subsequent spontaneous seizures.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Epilepsy, Non-convulsive status epilepticus, Peripheral immune response, Seizures, Serum, Spleen
in
Frontiers in Neurology
volume
10
issue
JUL
publisher
Frontiers
external identifiers
  • scopus:85069794164
ISSN
1664-2295
DOI
10.3389/fneur.2019.00701
language
English
LU publication?
yes
id
a36f51c0-5763-4780-855b-d919fc9e4332
date added to LUP
2019-08-23 11:35:58
date last changed
2019-08-28 04:58:26
@article{a36f51c0-5763-4780-855b-d919fc9e4332,
  abstract     = {<p>Non-convulsive status epilepticus (NCSE) is a prolonged epileptic seizure with subtle symptoms that may delay clinical diagnosis. Emerging experimental evidence shows brain pathology and epilepsy development following NCSE. New diagnostic/prognostic tools are therefore needed for earlier and better stratification of treatment. Here we examined whether NCSE initiates a peripheral immune response in blood serum from rats that experienced electrically-induced NCSE. ELISA analysis showed an acute transient increase in serum protein levels including interleukin-6 6 h post-NCSE, similar to the immune reaction in the brain. At 4 weeks post-NCSE, when 75% of rats subjected to NCSE had also developed spontaneous seizures, several immune proteins were altered. In particular, markers associated with microglia, macrophages and antigen presenting cells, such as CD68, MHCII, and galectin-3, were increased and the T-cell marker CD4 was decreased in serum compared to both non-stimulated controls and NCSE rats without spontaneous seizures, without correlation to interictal epileptiform activity. Analyses of serum following intracerebral injection of lipopolysaccharide (LPS) showed an acute increase in interleukin-6, but at 4 weeks unaltered levels of MHCII and galectin-3, an increase in CD8 and CD11b and a decrease in CD68. None of the increased serum protein levels after NCSE or LPS could be confirmed in spleen tissue. Our data identifies the possibility to detect peripheral changes in serum protein levels following NCSE, which may be related to the development of subsequent spontaneous seizures.</p>},
  articleno    = {701},
  author       = {Avdic, Una and Ahl, Matilda and Öberg, Maria and Ekdahl, Christine T.},
  issn         = {1664-2295},
  keyword      = {Epilepsy,Non-convulsive status epilepticus,Peripheral immune response,Seizures,Serum,Spleen},
  language     = {eng},
  month        = {01},
  number       = {JUL},
  publisher    = {Frontiers},
  series       = {Frontiers in Neurology},
  title        = {Immune profile in blood following non-convulsive epileptic seizures in rats},
  url          = {http://dx.doi.org/10.3389/fneur.2019.00701},
  volume       = {10},
  year         = {2019},
}