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Somatic mutation of immunoglobulin V genes in vitro

Källberg, Eva LU ; Jainandunsing, Sandra ; Gray, David and Leanderson, Tomas LU (1996) In Science (New York, N.Y.) 271(5253). p.9-1285
Abstract

The molecular mechanism behind affinity maturation is the introduction of point mutations in immunoglobulin (Ig) V genes, followed by the selective proliferation of B cells expressing mutants with increased affinity for antigen. An in vitro culture system was developed in which somatic hypermutation of Ig V genes was sustained in primed B cells. Cognate T cell help and cross-linking of the surface Ig were required, whereas the addition of lipopolysaccharide or a CD40 ligand to drive proliferation was insufficient. This system should facilitate understanding of the molecular and cellular mechanisms that regulate somatic mutation and B cell selection.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Amino Acid Sequence, Animals, B-Lymphocytes/immunology, Base Sequence, CD40 Antigens, CD40 Ligand, Cells, Cultured, Coculture Techniques, Genes, Immunoglobulin, Haptens/immunology, Hybridomas, Immunoglobulin Variable Region/genetics, Lipopolysaccharides/pharmacology, Membrane Glycoproteins/metabolism, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Mutation, Ovalbumin/immunology, Oxazolone/analogs & derivatives, Receptors, Antigen, B-Cell/immunology, Th2 Cells/immunology, Transfection
in
Science (New York, N.Y.)
volume
271
issue
5253
pages
9 - 1285
publisher
American Association for the Advancement of Science (AAAS)
external identifiers
  • scopus:0029879794
  • pmid:8638111
ISSN
0036-8075
DOI
10.1126/science.271.5253.1285
language
English
LU publication?
yes
id
a5263569-87e7-4ad6-ae8f-1865c22b4e9b
date added to LUP
2021-10-07 10:46:01
date last changed
2024-01-05 17:48:00
@article{a5263569-87e7-4ad6-ae8f-1865c22b4e9b,
  abstract     = {{<p>The molecular mechanism behind affinity maturation is the introduction of point mutations in immunoglobulin (Ig) V genes, followed by the selective proliferation of B cells expressing mutants with increased affinity for antigen. An in vitro culture system was developed in which somatic hypermutation of Ig V genes was sustained in primed B cells. Cognate T cell help and cross-linking of the surface Ig were required, whereas the addition of lipopolysaccharide or a CD40 ligand to drive proliferation was insufficient. This system should facilitate understanding of the molecular and cellular mechanisms that regulate somatic mutation and B cell selection.</p>}},
  author       = {{Källberg, Eva and Jainandunsing, Sandra and Gray, David and Leanderson, Tomas}},
  issn         = {{0036-8075}},
  keywords     = {{Amino Acid Sequence; Animals; B-Lymphocytes/immunology; Base Sequence; CD40 Antigens; CD40 Ligand; Cells, Cultured; Coculture Techniques; Genes, Immunoglobulin; Haptens/immunology; Hybridomas; Immunoglobulin Variable Region/genetics; Lipopolysaccharides/pharmacology; Membrane Glycoproteins/metabolism; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Mutation; Ovalbumin/immunology; Oxazolone/analogs & derivatives; Receptors, Antigen, B-Cell/immunology; Th2 Cells/immunology; Transfection}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{5253}},
  pages        = {{9--1285}},
  publisher    = {{American Association for the Advancement of Science (AAAS)}},
  series       = {{Science (New York, N.Y.)}},
  title        = {{Somatic mutation of immunoglobulin V genes in vitro}},
  url          = {{http://dx.doi.org/10.1126/science.271.5253.1285}},
  doi          = {{10.1126/science.271.5253.1285}},
  volume       = {{271}},
  year         = {{1996}},
}