Respiratory burst oxidases and apoplastic peroxidases facilitate ammonium syndrome development in Arabidopsis
(2021) In Environmental and Experimental Botany 181.- Abstract
Ammonium-nitrogen (NH4+) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH4+ nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH4+ conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH4+ as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and... (More)
Ammonium-nitrogen (NH4+) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH4+ nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH4+ conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH4+ as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and does not activate typical intracellular signalling pathways. In addition, elevated superoxide dismutase and apoplastic secretory peroxidase activities contributed to hydrogen peroxide (H2O2) accumulation in plants exposed to NH4+ nutrition. Consequently, higher H2O2 contents were determined in the extracellular space and were localised cytochemically. H2O2 is a substrate for cell wall cross-linking peroxidases, which showed enhanced activity in the presence of NH4+. Increase of cell wall polymerisation, could in turn inhibit cell elongation and slow down growth, as observed under NH4+ toxicity.
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- author
- Podgórska, Anna ; Burian, Maria ; Dobrzyńska, Katarzyna ; Rasmusson, Allan G. LU and Szal, Bożena
- organization
- publishing date
- 2021-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ammonium toxicity, Apoplastic reactive oxygen species, Cell wall peroxidase, Cell wall polymerisation, Respiratory burst oxidase homolog, Stress perception
- in
- Environmental and Experimental Botany
- volume
- 181
- article number
- 104279
- publisher
- Elsevier
- external identifiers
-
- scopus:85092202151
- ISSN
- 0098-8472
- DOI
- 10.1016/j.envexpbot.2020.104279
- language
- English
- LU publication?
- yes
- id
- a6045550-b4b8-472a-8bc2-dffc88bf28ba
- date added to LUP
- 2020-10-26 12:48:46
- date last changed
- 2024-05-15 19:26:20
@article{a6045550-b4b8-472a-8bc2-dffc88bf28ba, abstract = {{<p>Ammonium-nitrogen (NH<sub>4</sub><sup>+</sup>) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH<sub>4</sub><sup>+</sup> nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH<sub>4</sub><sup>+</sup> conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH<sub>4</sub><sup>+</sup> as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and does not activate typical intracellular signalling pathways. In addition, elevated superoxide dismutase and apoplastic secretory peroxidase activities contributed to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) accumulation in plants exposed to NH<sub>4</sub><sup>+</sup> nutrition. Consequently, higher H<sub>2</sub>O<sub>2</sub> contents were determined in the extracellular space and were localised cytochemically. H<sub>2</sub>O<sub>2</sub> is a substrate for cell wall cross-linking peroxidases, which showed enhanced activity in the presence of NH<sub>4</sub><sup>+</sup>. Increase of cell wall polymerisation, could in turn inhibit cell elongation and slow down growth, as observed under NH<sub>4</sub><sup>+</sup> toxicity.</p>}}, author = {{Podgórska, Anna and Burian, Maria and Dobrzyńska, Katarzyna and Rasmusson, Allan G. and Szal, Bożena}}, issn = {{0098-8472}}, keywords = {{Ammonium toxicity; Apoplastic reactive oxygen species; Cell wall peroxidase; Cell wall polymerisation; Respiratory burst oxidase homolog; Stress perception}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Environmental and Experimental Botany}}, title = {{Respiratory burst oxidases and apoplastic peroxidases facilitate ammonium syndrome development in Arabidopsis}}, url = {{http://dx.doi.org/10.1016/j.envexpbot.2020.104279}}, doi = {{10.1016/j.envexpbot.2020.104279}}, volume = {{181}}, year = {{2021}}, }