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Respiratory burst oxidases and apoplastic peroxidases facilitate ammonium syndrome development in Arabidopsis

Podgórska, Anna ; Burian, Maria ; Dobrzyńska, Katarzyna ; Rasmusson, Allan G. LU and Szal, Bożena (2021) In Environmental and Experimental Botany 181.
Abstract

Ammonium-nitrogen (NH4+) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH4+ nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH4+ conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH4+ as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and... (More)

Ammonium-nitrogen (NH4+) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH4+ nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH4+ conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH4+ as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and does not activate typical intracellular signalling pathways. In addition, elevated superoxide dismutase and apoplastic secretory peroxidase activities contributed to hydrogen peroxide (H2O2) accumulation in plants exposed to NH4+ nutrition. Consequently, higher H2O2 contents were determined in the extracellular space and were localised cytochemically. H2O2 is a substrate for cell wall cross-linking peroxidases, which showed enhanced activity in the presence of NH4+. Increase of cell wall polymerisation, could in turn inhibit cell elongation and slow down growth, as observed under NH4+ toxicity.

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organization
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type
Contribution to journal
publication status
published
subject
keywords
Ammonium toxicity, Apoplastic reactive oxygen species, Cell wall peroxidase, Cell wall polymerisation, Respiratory burst oxidase homolog, Stress perception
in
Environmental and Experimental Botany
volume
181
article number
104279
publisher
Elsevier
external identifiers
  • scopus:85092202151
ISSN
0098-8472
DOI
10.1016/j.envexpbot.2020.104279
language
English
LU publication?
yes
id
a6045550-b4b8-472a-8bc2-dffc88bf28ba
date added to LUP
2020-10-26 12:48:46
date last changed
2024-05-15 19:26:20
@article{a6045550-b4b8-472a-8bc2-dffc88bf28ba,
  abstract     = {{<p>Ammonium-nitrogen (NH<sub>4</sub><sup>+</sup>) nutrition is linked to metabolic over-reduction for plants. The characteristic symptom of sole NH<sub>4</sub><sup>+</sup> nutrition is growth suppression, signifying this condition as the ammonium syndrome. In the present study, we investigated the mechanism of perception of high NH<sub>4</sub><sup>+</sup> conditions in Arabidopsis thaliana plants by examining apoplastic reactive oxygen species (ROS) metabolism. Major enzyme activity and a special pattern of expression of NADPH-dependent respiratory burst oxidases (RBOH) was found in Arabidopsis individuals cultured under NH<sub>4</sub><sup>+</sup> as the sole nitrogen source. This oxidative burst is independent of RBOHD/F expression and does not activate typical intracellular signalling pathways. In addition, elevated superoxide dismutase and apoplastic secretory peroxidase activities contributed to hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) accumulation in plants exposed to NH<sub>4</sub><sup>+</sup> nutrition. Consequently, higher H<sub>2</sub>O<sub>2</sub> contents were determined in the extracellular space and were localised cytochemically. H<sub>2</sub>O<sub>2</sub> is a substrate for cell wall cross-linking peroxidases, which showed enhanced activity in the presence of NH<sub>4</sub><sup>+</sup>. Increase of cell wall polymerisation, could in turn inhibit cell elongation and slow down growth, as observed under NH<sub>4</sub><sup>+</sup> toxicity.</p>}},
  author       = {{Podgórska, Anna and Burian, Maria and Dobrzyńska, Katarzyna and Rasmusson, Allan G. and Szal, Bożena}},
  issn         = {{0098-8472}},
  keywords     = {{Ammonium toxicity; Apoplastic reactive oxygen species; Cell wall peroxidase; Cell wall polymerisation; Respiratory burst oxidase homolog; Stress perception}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Environmental and Experimental Botany}},
  title        = {{Respiratory burst oxidases and apoplastic peroxidases facilitate ammonium syndrome development in Arabidopsis}},
  url          = {{http://dx.doi.org/10.1016/j.envexpbot.2020.104279}},
  doi          = {{10.1016/j.envexpbot.2020.104279}},
  volume       = {{181}},
  year         = {{2021}},
}