Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons
(1994) In Brain Research 641(1). p.76-82- Abstract
We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of... (More)
We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of culturing. Furthermore, both insulin and IGF-II were found to inhibit proliferation of support cells in the outgrowth region in a manner suggesting effects via their individual receptors. The inhibition, about 30%, was observable after 4 but not 11 days in culture. It is not clear if this reflects a stimulated differentiation of some cells. By contrast, IGF-I lacked effects on both regeneration and proliferation. In conclusion, the results suggest that insulin and IGF-II are involved in the regulation of peripheral nerve regeneration.
(Less)
- author
- Edbladh, M. LU ; Fex-Svenningsen, Å LU ; Ekström, Per LU and Edström, A. LU
- organization
- publishing date
- 1994-03-28
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Growth factor, Inhibition, Proliferation, Protein synthesis, Sciatic nerve
- in
- Brain Research
- volume
- 641
- issue
- 1
- pages
- 76 - 82
- publisher
- Elsevier
- external identifiers
-
- scopus:0028267494
- pmid:8019854
- ISSN
- 0006-8993
- DOI
- 10.1016/0006-8993(94)91817-1
- language
- English
- LU publication?
- yes
- id
- a666a9d8-bb65-4563-8652-a6410cc62d28
- date added to LUP
- 2016-12-07 14:42:50
- date last changed
- 2025-01-12 17:07:12
@article{a666a9d8-bb65-4563-8652-a6410cc62d28,
abstract = {{<p>We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of culturing. Furthermore, both insulin and IGF-II were found to inhibit proliferation of support cells in the outgrowth region in a manner suggesting effects via their individual receptors. The inhibition, about 30%, was observable after 4 but not 11 days in culture. It is not clear if this reflects a stimulated differentiation of some cells. By contrast, IGF-I lacked effects on both regeneration and proliferation. In conclusion, the results suggest that insulin and IGF-II are involved in the regulation of peripheral nerve regeneration.</p>}},
author = {{Edbladh, M. and Fex-Svenningsen, Å and Ekström, Per and Edström, A.}},
issn = {{0006-8993}},
keywords = {{Growth factor; Inhibition; Proliferation; Protein synthesis; Sciatic nerve}},
language = {{eng}},
month = {{03}},
number = {{1}},
pages = {{76--82}},
publisher = {{Elsevier}},
series = {{Brain Research}},
title = {{Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons}},
url = {{http://dx.doi.org/10.1016/0006-8993(94)91817-1}},
doi = {{10.1016/0006-8993(94)91817-1}},
volume = {{641}},
year = {{1994}},
}