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Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons

Edbladh, M. LU ; Fex-Svenningsen, Å LU ; Ekström, Per LU and Edström, A. LU (1994) In Brain Research 641(1). p.76-82
Abstract

We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of... (More)

We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of culturing. Furthermore, both insulin and IGF-II were found to inhibit proliferation of support cells in the outgrowth region in a manner suggesting effects via their individual receptors. The inhibition, about 30%, was observable after 4 but not 11 days in culture. It is not clear if this reflects a stimulated differentiation of some cells. By contrast, IGF-I lacked effects on both regeneration and proliferation. In conclusion, the results suggest that insulin and IGF-II are involved in the regulation of peripheral nerve regeneration.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Growth factor, Inhibition, Proliferation, Protein synthesis, Sciatic nerve
in
Brain Research
volume
641
issue
1
pages
76 - 82
publisher
Elsevier
external identifiers
  • scopus:0028267494
ISSN
0006-8993
DOI
10.1016/0006-8993(94)91817-1
language
English
LU publication?
yes
id
a666a9d8-bb65-4563-8652-a6410cc62d28
date added to LUP
2016-12-07 14:42:50
date last changed
2017-01-27 16:11:40
@article{a666a9d8-bb65-4563-8652-a6410cc62d28,
  abstract     = {<p>We used the in vitro regenerating frog sciatic nerve to look for effects of insulin and insulin-like growth factors I and II (IGF-I, IGF-II) on regeneration of sensory axons and on injury induced support cell proliferation in the outgrowth region. In nerves cultured for 11 days, a physiological dose (10 ng/ml, ≈ nM) of insulin or IGF-II increased ganglionic protein synthesis (by 20% and 50%, respectively) as well as the level of newly formed, radiolabelled axonal material distal to a crush injury (both by 80%), compared to untreated, paired controls. In addition, insulin increased the outgrowth distance of the furthest regenerating sensory axons by 10%. The preparation was particularly sensitive to insulin during the first 5 days of culturing. Furthermore, both insulin and IGF-II were found to inhibit proliferation of support cells in the outgrowth region in a manner suggesting effects via their individual receptors. The inhibition, about 30%, was observable after 4 but not 11 days in culture. It is not clear if this reflects a stimulated differentiation of some cells. By contrast, IGF-I lacked effects on both regeneration and proliferation. In conclusion, the results suggest that insulin and IGF-II are involved in the regulation of peripheral nerve regeneration.</p>},
  author       = {Edbladh, M. and Fex-Svenningsen, Å and Ekström, Per and Edström, A.},
  issn         = {0006-8993},
  keyword      = {Growth factor,Inhibition,Proliferation,Protein synthesis,Sciatic nerve},
  language     = {eng},
  month        = {03},
  number       = {1},
  pages        = {76--82},
  publisher    = {Elsevier},
  series       = {Brain Research},
  title        = {Insulin and IGF-II, but not IGF-I, stimulate the in vitro regeneration of adult frog sciatic sensory axons},
  url          = {http://dx.doi.org/10.1016/0006-8993(94)91817-1},
  volume       = {641},
  year         = {1994},
}