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A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27-IgMhigh B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation

Bemark, Mats LU orcid ; Friskopp, Linda ; Saghafian-Hedengren, Shanie ; Koethe, Susanne ; Fasth, Anders ; Abrahamsson, Jonas ; Sverremark-Ekström, Eva ; Andersson, Bengt A. and Mellgren, Karin (2013) In Clinical Immunology 149(3 Part B). p.421-431
Abstract

The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27+ B cells formed after transplantation with the number of CD27+IgMhigh cells more affected than class-switched ones. A previously unacknowledged population of CD27-IgMhigh cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27-IgMhigh B cells expressed markers typical for transitional B cells, and the non-transitional CD27-IgMhigh cells could be further... (More)

The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27+ B cells formed after transplantation with the number of CD27+IgMhigh cells more affected than class-switched ones. A previously unacknowledged population of CD27-IgMhigh cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27-IgMhigh B cells expressed markers typical for transitional B cells, and the non-transitional CD27-IgMhigh cells could be further divided into subpopulations based on their ability to extrude the dye Rhodamine 123 and their expression of CD45RBMEM55, a glycosylation-dependent epitope. Thus, we define several novel human CD27-IgMhigh B cell subpopulations in blood, all of which are present in higher frequencies and numbers in young children and after HSCT than in adults.

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author
; ; ; ; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
keywords
B cell, Hematopoietic stem cell transplantation, Immunological ontogeny, Lymphocyte development
in
Clinical Immunology
volume
149
issue
3 Part B
pages
421 - 431
publisher
Elsevier
external identifiers
  • pmid:24211716
  • scopus:84887151419
ISSN
1521-6616
DOI
10.1016/j.clim.2013.08.011
language
English
LU publication?
no
id
a67dbacc-410e-4caf-9b21-d0503994adc0
date added to LUP
2023-12-06 17:09:51
date last changed
2024-01-04 15:32:43
@article{a67dbacc-410e-4caf-9b21-d0503994adc0,
  abstract     = {{<p>The immune system is dysfunctional for years after hematopoietic stem cell transplantation (HSCT). A potential cause is an intrinsic B cell deficiency. In a cohort of pediatric HSCT patients few CD27<sup>+</sup> B cells formed after transplantation with the number of CD27<sup>+</sup>IgM<sup>high</sup> cells more affected than class-switched ones. A previously unacknowledged population of CD27<sup>-</sup>IgM<sup>high</sup> cells made up the majority of B cells and this population was also enlarged in healthy children compared to adults. Only a minority of these CD27<sup>-</sup>IgM<sup>high</sup> B cells expressed markers typical for transitional B cells, and the non-transitional CD27<sup>-</sup>IgM<sup>high</sup> cells could be further divided into subpopulations based on their ability to extrude the dye Rhodamine 123 and their expression of CD45RB<sup>MEM55</sup>, a glycosylation-dependent epitope. Thus, we define several novel human CD27<sup>-</sup>IgM<sup>high</sup> B cell subpopulations in blood, all of which are present in higher frequencies and numbers in young children and after HSCT than in adults.</p>}},
  author       = {{Bemark, Mats and Friskopp, Linda and Saghafian-Hedengren, Shanie and Koethe, Susanne and Fasth, Anders and Abrahamsson, Jonas and Sverremark-Ekström, Eva and Andersson, Bengt A. and Mellgren, Karin}},
  issn         = {{1521-6616}},
  keywords     = {{B cell; Hematopoietic stem cell transplantation; Immunological ontogeny; Lymphocyte development}},
  language     = {{eng}},
  number       = {{3 Part B}},
  pages        = {{421--431}},
  publisher    = {{Elsevier}},
  series       = {{Clinical Immunology}},
  title        = {{A glycosylation-dependent CD45RB epitope defines previously unacknowledged CD27<sup>-</sup>IgM<sup>high</sup> B cell subpopulations enriched in young children and after hematopoietic stem cell transplantation}},
  url          = {{http://dx.doi.org/10.1016/j.clim.2013.08.011}},
  doi          = {{10.1016/j.clim.2013.08.011}},
  volume       = {{149}},
  year         = {{2013}},
}