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Targeted uptake of folic acid-functionalized iron oxide nanoparticles by ovarian cancer cells in the presence but not in the absence of serum

Krais, Annette LU ; Wortmann, Laura; Hermanns, Laura; Feliu, Neus; Vahter, Marie; Stucky, Stefan; Mathur, Sanjay and Fadeel, Bengt (2014) In Nanomedicine: Nanotechnology, Biology, and Medicine 10(7). p.31-1421
Abstract

Targeted delivery of nanoparticles to cells or tissues of interest is arguably the "holy grail" of nanomedicine. Using primary human macrophages and ovarian cancer cells, we evaluated the biocompatibility and specific targeting of folic acid (FA)-conjugated iron oxide nanoparticles with organic [poly(ethylene glycol), PEG] or inorganic (SiO2) intermediate surface coatings. Reduction of folate receptor-α expression using specific siRNA resulted in a significant decrease in cellular uptake of the SiO2-coated nanoparticles, but did not affect uptake of PEG-coated nanoparticles. Notably, specific (i.e. FA-dependent) uptake was observed only in the presence of serum proteins. The strategy presented here for receptor-mediated uptake of... (More)

Targeted delivery of nanoparticles to cells or tissues of interest is arguably the "holy grail" of nanomedicine. Using primary human macrophages and ovarian cancer cells, we evaluated the biocompatibility and specific targeting of folic acid (FA)-conjugated iron oxide nanoparticles with organic [poly(ethylene glycol), PEG] or inorganic (SiO2) intermediate surface coatings. Reduction of folate receptor-α expression using specific siRNA resulted in a significant decrease in cellular uptake of the SiO2-coated nanoparticles, but did not affect uptake of PEG-coated nanoparticles. Notably, specific (i.e. FA-dependent) uptake was observed only in the presence of serum proteins. The strategy presented here for receptor-mediated uptake of nanoparticles with pre-defined surface chemistry may enable targeting of nanoparticles for therapeutic and imaging applications. From the clinical editor: In this study the receptor specific uptake of folic acid-functionalized iron oxide nanoparticles was determined in ovarian cancer cells. It was found that the presence of serum proteins is an absolute requirement for the uptake of these nanoparticles. The described strategy for receptor-mediated uptake of nanoparticles with pre-defined surface chemistry may enable a better targeting of nanoparticles for additional therapeutic and imaging applications.

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published
keywords
Blood, Cell Line, Tumor, Female, Ferric Compounds, Flow Cytometry, Folic Acid, Humans, Metal Nanoparticles, Microscopy, Electron, Scanning, Microscopy, Electron, Transmission, Ovarian Neoplasms, RNA, Small Interfering, X-Ray Diffraction, Journal Article, Research Support, Non-U.S. Gov't
in
Nanomedicine: Nanotechnology, Biology, and Medicine
volume
10
issue
7
pages
11 pages
publisher
Elsevier
external identifiers
  • scopus:84908503564
ISSN
1549-9642
DOI
10.1016/j.nano.2014.01.006
language
English
LU publication?
no
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a70fc3ff-aa63-4d6c-8b77-f67de0cc69f8
date added to LUP
2017-10-17 15:30:12
date last changed
2017-10-30 15:42:15
@article{a70fc3ff-aa63-4d6c-8b77-f67de0cc69f8,
  abstract     = {<p>Targeted delivery of nanoparticles to cells or tissues of interest is arguably the "holy grail" of nanomedicine. Using primary human macrophages and ovarian cancer cells, we evaluated the biocompatibility and specific targeting of folic acid (FA)-conjugated iron oxide nanoparticles with organic [poly(ethylene glycol), PEG] or inorganic (SiO2) intermediate surface coatings. Reduction of folate receptor-α expression using specific siRNA resulted in a significant decrease in cellular uptake of the SiO2-coated nanoparticles, but did not affect uptake of PEG-coated nanoparticles. Notably, specific (i.e. FA-dependent) uptake was observed only in the presence of serum proteins. The strategy presented here for receptor-mediated uptake of nanoparticles with pre-defined surface chemistry may enable targeting of nanoparticles for therapeutic and imaging applications. From the clinical editor: In this study the receptor specific uptake of folic acid-functionalized iron oxide nanoparticles was determined in ovarian cancer cells. It was found that the presence of serum proteins is an absolute requirement for the uptake of these nanoparticles. The described strategy for receptor-mediated uptake of nanoparticles with pre-defined surface chemistry may enable a better targeting of nanoparticles for additional therapeutic and imaging applications.</p>},
  author       = {Krais, Annette and Wortmann, Laura and Hermanns, Laura and Feliu, Neus and Vahter, Marie and Stucky, Stefan and Mathur, Sanjay and Fadeel, Bengt},
  issn         = {1549-9642},
  keyword      = {Blood,Cell Line, Tumor,Female,Ferric Compounds,Flow Cytometry,Folic Acid,Humans,Metal Nanoparticles,Microscopy, Electron, Scanning,Microscopy, Electron, Transmission,Ovarian Neoplasms,RNA, Small Interfering,X-Ray Diffraction,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {7},
  pages        = {31--1421},
  publisher    = {Elsevier},
  series       = {Nanomedicine: Nanotechnology, Biology, and Medicine},
  title        = {Targeted uptake of folic acid-functionalized iron oxide nanoparticles by ovarian cancer cells in the presence but not in the absence of serum},
  url          = {http://dx.doi.org/10.1016/j.nano.2014.01.006},
  volume       = {10},
  year         = {2014},
}