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What do photons do to fluorescently stained DNA in confinement?

Beech, J. P. LU ; Nyberg, Lena LU ; Fritzsche, Joachim; Westerlund, Fredrik and Tegenfeldt, J. O. LU (2013) 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 1. p.5-7
Abstract

We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.

Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
Confinement, DNA, Intercalating dye, Nano-channels, Photobleaching
host publication
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
volume
1
pages
3 pages
publisher
Chemical and Biological Microsystems Society
conference name
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
conference location
Freiburg, Germany
conference dates
2013-10-27 - 2013-10-31
external identifiers
  • scopus:84907318000
ISBN
9781632666246
language
English
LU publication?
yes
id
a7cd51af-9595-496d-baae-f5aa98b2e8d0
date added to LUP
2018-10-20 08:44:49
date last changed
2019-04-12 09:35:50
@inproceedings{a7cd51af-9595-496d-baae-f5aa98b2e8d0,
  abstract     = {<p>We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.</p>},
  author       = {Beech, J. P. and Nyberg, Lena and Fritzsche, Joachim and Westerlund, Fredrik and Tegenfeldt, J. O.},
  isbn         = {9781632666246},
  keyword      = {Confinement,DNA,Intercalating dye,Nano-channels,Photobleaching},
  language     = {eng},
  location     = {Freiburg, Germany},
  month        = {01},
  pages        = {5--7},
  publisher    = {Chemical and Biological Microsystems Society},
  title        = {What do photons do to fluorescently stained DNA in confinement?},
  volume       = {1},
  year         = {2013},
}