What do photons do to fluorescently stained DNA in confinement?
Beech, J. P. LU ; Nyberg, Lena LU ; Fritzsche, Joachim ; Westerlund, Fredrik and Tegenfeldt, J. O. LU
(2013)
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
1.
p.5-7
- Abstract
We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.
Please use this url to cite or link to this publication:
http://lup.lub.lu.se/record/a7cd51af-9595-496d-baae-f5aa98b2e8d0
- author
- Beech, J. P.
LU
; Nyberg, Lena
LU
; Fritzsche, Joachim
; Westerlund, Fredrik
and Tegenfeldt, J. O.
LU
- organization
- publishing date
- 2013-01-01
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- Confinement, DNA, Intercalating dye, Nano-channels, Photobleaching
- host publication
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- volume
- 1
- pages
- 3 pages
- publisher
- Chemical and Biological Microsystems Society
- conference name
- 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
- conference location
- Freiburg, Germany
- conference dates
- 2013-10-27 - 2013-10-31
- external identifiers
-
- scopus:84907318000
- ISBN
- 9781632666246
- language
- English
- LU publication?
- yes
- id
- a7cd51af-9595-496d-baae-f5aa98b2e8d0
- date added to LUP
- 2018-10-20 08:44:49
- date last changed
- 2020-01-16 11:15:42
@inproceedings{a7cd51af-9595-496d-baae-f5aa98b2e8d0,
abstract = {<p>We have studied a selection of factors influencing the damage of DNA in nanochannels during fluorescence imaging. For cutting and nicking of DNA we show that the DNA is shortened during imaging. To avoid photodamage over the course of several hours of a typical experiment, we demonstrate the importance of an oxygen free gas to propel the buffer solution through the device. Finally, by varying the size of the channels, we show indications that higher DNA concentrations lead to higher rates of photodamage necessitating a balance between needs for highly stretched DNA and needs for long measurement times.</p>},
author = {Beech, J. P. and Nyberg, Lena and Fritzsche, Joachim and Westerlund, Fredrik and Tegenfeldt, J. O.},
booktitle = {17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013},
isbn = {9781632666246},
language = {eng},
month = {01},
pages = {5--7},
publisher = {Chemical and Biological Microsystems Society},
title = {What do photons do to fluorescently stained DNA in confinement?},
volume = {1},
year = {2013},
}