Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application
(1982) In Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology 90(6). p.423-433- Abstract
- Antibody response to Campylobacter jejuni/coli (CJC) was investigated, using an enzyme-linked immunosorbent assay, ELISA. With a mixture of lipopolysaccharide from two CJC strains as antigen in ELISA, all 24 tested rabbit anti-CJC sera showed high antibody levels. However, only 70% of sera from patients with Campylobacter enteritis demonstrated an antibody response against the combined LPS antigen, using paired sera. In addition, the results obtained suggested non-specific binding of human immunoglobulin. When 24 formalinized whole CJC bacteria were used as antigen in ELISA, all corresponding rabbit antisera reacted with one strain (M 14). Essentially no unspecific binding of human immunoglobulin was obtained. Antibodies were detected in... (More)
- Antibody response to Campylobacter jejuni/coli (CJC) was investigated, using an enzyme-linked immunosorbent assay, ELISA. With a mixture of lipopolysaccharide from two CJC strains as antigen in ELISA, all 24 tested rabbit anti-CJC sera showed high antibody levels. However, only 70% of sera from patients with Campylobacter enteritis demonstrated an antibody response against the combined LPS antigen, using paired sera. In addition, the results obtained suggested non-specific binding of human immunoglobulin. When 24 formalinized whole CJC bacteria were used as antigen in ELISA, all corresponding rabbit antisera reacted with one strain (M 14). Essentially no unspecific binding of human immunoglobulin was obtained. Antibodies were detected in sera from healthy blood donors and at a lower level in sera from children, suggesting early immunization. In 67 enteritis patients with positive stool cultures for CJC, a significantly increased level of IgG antibodies could be detected in single or paired serum samples from 82% of the patients. An IgG titre increase occurred early in the course of infection, suggesting a boosting of an earlier immunization. IgM antibodies could be detected in the same sera in 77% of the patients. Considering both IgG and IgM analyses of the enteritis sera, 94% of the patients were positive in Campylobacter ELISA serology compared with only 5% of healthy controls. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1102979
- author
- Walder, Mats LU and Forsgren, Arne LU
- organization
- publishing date
- 1982
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology
- volume
- 90
- issue
- 6
- pages
- 423 - 433
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:7168317
- scopus:0020464760
- ISSN
- 0108-0180
- language
- English
- LU publication?
- yes
- id
- a7deac69-415d-4575-b2b2-84e97eaec4dd (old id 1102979)
- date added to LUP
- 2016-04-01 16:26:28
- date last changed
- 2021-01-24 04:59:43
@article{a7deac69-415d-4575-b2b2-84e97eaec4dd,
abstract = {{Antibody response to Campylobacter jejuni/coli (CJC) was investigated, using an enzyme-linked immunosorbent assay, ELISA. With a mixture of lipopolysaccharide from two CJC strains as antigen in ELISA, all 24 tested rabbit anti-CJC sera showed high antibody levels. However, only 70% of sera from patients with Campylobacter enteritis demonstrated an antibody response against the combined LPS antigen, using paired sera. In addition, the results obtained suggested non-specific binding of human immunoglobulin. When 24 formalinized whole CJC bacteria were used as antigen in ELISA, all corresponding rabbit antisera reacted with one strain (M 14). Essentially no unspecific binding of human immunoglobulin was obtained. Antibodies were detected in sera from healthy blood donors and at a lower level in sera from children, suggesting early immunization. In 67 enteritis patients with positive stool cultures for CJC, a significantly increased level of IgG antibodies could be detected in single or paired serum samples from 82% of the patients. An IgG titre increase occurred early in the course of infection, suggesting a boosting of an earlier immunization. IgM antibodies could be detected in the same sera in 77% of the patients. Considering both IgG and IgM analyses of the enteritis sera, 94% of the patients were positive in Campylobacter ELISA serology compared with only 5% of healthy controls.}},
author = {{Walder, Mats and Forsgren, Arne}},
issn = {{0108-0180}},
language = {{eng}},
number = {{6}},
pages = {{423--433}},
publisher = {{John Wiley & Sons Inc.}},
series = {{Acta pathologica, microbiologica, et immunologica Scandinavica. Section B, Microbiology}},
title = {{Enzyme-linked immunosorbent assay (ELISA) for antibodies against Campylobacter jejuni, and its clinical application}},
volume = {{90}},
year = {{1982}},
}