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Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.

Andersson, Alexandra LU ; Edlund, Ulrica ; Sjöberg, John ; Albertsson, Ann-Christine and Stålbrand, Henrik LU (2008) In Biomacromolecules 9(8). p.2104-2110
Abstract
O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95%... (More)
O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery. (Less)
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author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biomacromolecules
volume
9
issue
8
pages
2104 - 2110
publisher
The American Chemical Society (ACS)
external identifiers
  • wos:000258400200003
  • pmid:18590309
  • scopus:52649167335
ISSN
1526-4602
DOI
10.1021/bm701399m
language
English
LU publication?
yes
id
a7ef8330-b365-4fdf-ba81-896cb5a04f70 (old id 1181741)
date added to LUP
2016-04-01 12:27:28
date last changed
2022-02-18 22:49:25
@article{a7ef8330-b365-4fdf-ba81-896cb5a04f70,
  abstract     = {{O-Acetyl-galactoglucomannan (AcGGM) is the major soft-wood hemicellulose. Structurally modified AcGGM and hydrogels of AcGGM were prepared. The degree of substitution (DS) of AcGGM was modified enzymatically with alpha-galactosidase, and chemically with an acrylate derivative, 2-hydroxyethylmethacrylate (HEMA). The hydrolysis of AcGGM with beta-mannanase was shown to increase with decreasing DS. AcGGM hydrogels were prepared from chemically modified AcGGM with varying DS of HEMA. Bovine serum albumin (BSA) was encapsulated in hydrogels. A spontaneous burst release of BSA was decreased with increased DS of HEMA. The addition of beta-mannanase significantly enhanced the BSA release from hydrogels with a DS of 0.36, reaching a maximum of 95% released BSA after eight hours compared to 60% without enzyme. Thus, both the pendant group composition and the enzyme action are valuable tools in the tailoring of hydrogel release profiles of potential interest for intestine drug delivery.}},
  author       = {{Andersson, Alexandra and Edlund, Ulrica and Sjöberg, John and Albertsson, Ann-Christine and Stålbrand, Henrik}},
  issn         = {{1526-4602}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{2104--2110}},
  publisher    = {{The American Chemical Society (ACS)}},
  series       = {{Biomacromolecules}},
  title        = {{Protein Release from Galactoglucomannan Hydrogels: Influence of Substitutions and Enzymatic Hydrolysis by beta-Mannanase.}},
  url          = {{http://dx.doi.org/10.1021/bm701399m}},
  doi          = {{10.1021/bm701399m}},
  volume       = {{9}},
  year         = {{2008}},
}