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Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals

Shipovskov, Stepan LU ; Ferapontova, Elena LU ; Gazaryan, I and Ruzgas, Tautgirdas LU (2004) In Bioelectrochemistry 63(1-2). p.277-280
Abstract
The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the... (More)
The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bi-electrode system, Recombinant horseradish peroxidase, Cytochrome c, Hydrogen peroxide, Superoxide radical
in
Bioelectrochemistry
volume
63
issue
1-2
pages
277 - 280
publisher
Elsevier
external identifiers
  • wos:000221786900050
  • pmid:15110287
  • scopus:2042456719
  • pmid:15110287
ISSN
1878-562X
DOI
10.1016/j.bioelechem.2003.09.026
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Biochemistry and Structural Biology (S) (000006142), Analytical Chemistry (S/LTH) (011001004)
id
a874e76e-3f7d-4ee2-8739-5cbdfe327fd9 (old id 138522)
date added to LUP
2016-04-01 11:46:16
date last changed
2022-04-05 04:50:07
@article{a874e76e-3f7d-4ee2-8739-5cbdfe327fd9,
  abstract     = {{The reliable detection of a superoxide anion radical O-2(.-) - is complicated by its spontaneous dismutation reaction to H2O2 at acidic pHs. To simultaneously detect both O-2(.-) and H2O2 produced in the course of its spontaneous dismutation, an electrochemical two-electrode system based on cytochrome c (cyt c) and recombinant horseradish peroxidase (rHRP) was applied. Therewith, a limited applicability of the cyt c system for the reliable monitoring of O-2(.-) in acidic and neutral solutions was shown. It was demonstrated that both the reaction of O-2(.-) dismutation to H2O2 and the reaction between the formed H2O2 and O-2(.-) chemically decrease the amount of the initially present O-2(.-) decreasing the sensitivity and reliability of the electrochemical detection at acidic pH. However, by appropriately varying solution pH, the concentration of O-2(.-) - initially injected in the system can be estimated from the analysis of calibration curves for H2O2 obtained with highly sensitive rHRP-modified electrode system at pH 6.0 and 7.0. (C) 2004 Elsevier B.V. All rights reserved.}},
  author       = {{Shipovskov, Stepan and Ferapontova, Elena and Gazaryan, I and Ruzgas, Tautgirdas}},
  issn         = {{1878-562X}},
  keywords     = {{Bi-electrode system; Recombinant horseradish peroxidase; Cytochrome c; Hydrogen peroxide; Superoxide radical}},
  language     = {{eng}},
  number       = {{1-2}},
  pages        = {{277--280}},
  publisher    = {{Elsevier}},
  series       = {{Bioelectrochemistry}},
  title        = {{Recombinant horseradish peroxidase- and cytochrome c-based two-electrode system for detection of superoxide radicals}},
  url          = {{http://dx.doi.org/10.1016/j.bioelechem.2003.09.026}},
  doi          = {{10.1016/j.bioelechem.2003.09.026}},
  volume       = {{63}},
  year         = {{2004}},
}