The effects of lipophilic substances on the shape of erythrocytes demonstrated by a new in vitro-method
Söderberg, Lars LU ; Haag, Linnea ; Höglund, Peter LU ; Stenberg, Pål and Wahlgren, Marie LU
(2009)
In European Journal of Pharmaceutical Sciences
36(4-5).
p.458-464
- Abstract
- Abstract
Low aqueous solubility of lipophilic agents, such as free fatty acids, hampers proper in vitro demonstration of biological effects, yielding an ambiguous in vitro-in vivo correlation. We have therefore developed a method for evaluating the acute effects of lipophilic substances on the shape of erythrocytes and estimated EC50 and Hill coefficient according to the sigmoidal Emax model.
The test substance dissolved in medium-chain triglyceride is coated on a polycarbonate slide which serves as a cover sheet of a Bürker chamber. Freshly collected finger-tip blood is diluted with autologous EDTA-plasma and introduced into the chamber. After ten min at 37 C, the cells are photographed under... (More) - Abstract
Low aqueous solubility of lipophilic agents, such as free fatty acids, hampers proper in vitro demonstration of biological effects, yielding an ambiguous in vitro-in vivo correlation. We have therefore developed a method for evaluating the acute effects of lipophilic substances on the shape of erythrocytes and estimated EC50 and Hill coefficient according to the sigmoidal Emax model.
The test substance dissolved in medium-chain triglyceride is coated on a polycarbonate slide which serves as a cover sheet of a Bürker chamber. Freshly collected finger-tip blood is diluted with autologous EDTA-plasma and introduced into the chamber. After ten min at 37 C, the cells are photographed under microscope and the fractions of normal and defect cells are evaluated. No staining is needed and the cells are kept viable during the test period.
With increasing chain length, fatty acids, aliphatic amines and alcohols all increased the fraction of defect erythrocytes in a concentration-dependent manner. The results indicate that several fatty acids are very potent in their acute actions on erythrocytes, and that this effect is due to chain length rather than conformation.
Conclusion: The technique offers a screening method for testing the harmful effects of small amounts of lipophilic substances on erythrocytes. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1241394
- author
- Söderberg, Lars
LU
; Haag, Linnea
; Höglund, Peter
LU
; Stenberg, Pål
and Wahlgren, Marie
LU
- organization
- publishing date
- 2009
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Journal of Pharmaceutical Sciences
- volume
- 36
- issue
- 4-5
- pages
- 458 - 464
- publisher
- Elsevier
- external identifiers
-
- wos:000264008600010
- scopus:59349090288
- pmid:19103286
- ISSN
- 1879-0720
- DOI
- 10.1016/j.ejps.2008.11.011
- language
- English
- LU publication?
- yes
- additional info
- Under fliken "Context/Affiliation": "Hospital Pharmacy, University Hospital, Malmö" är den enhet där huvudelen av arbetet har utförts, tillsammans med "Food technology"
- id
- a881de00-56d1-43f8-81ef-21e79e958e10 (old id 1241394)
- date added to LUP
- 2016-04-01 11:40:07
- date last changed
- 2023-09-15 03:09:25
@article{a881de00-56d1-43f8-81ef-21e79e958e10,
abstract = {{Abstract<br/><br>
<br/><br>
Low aqueous solubility of lipophilic agents, such as free fatty acids, hampers proper in vitro demonstration of biological effects, yielding an ambiguous in vitro-in vivo correlation. We have therefore developed a method for evaluating the acute effects of lipophilic substances on the shape of erythrocytes and estimated EC50 and Hill coefficient according to the sigmoidal Emax model. <br/><br>
<br/><br>
The test substance dissolved in medium-chain triglyceride is coated on a polycarbonate slide which serves as a cover sheet of a Bürker chamber. Freshly collected finger-tip blood is diluted with autologous EDTA-plasma and introduced into the chamber. After ten min at 37 C, the cells are photographed under microscope and the fractions of normal and defect cells are evaluated. No staining is needed and the cells are kept viable during the test period.<br/><br>
<br/><br>
With increasing chain length, fatty acids, aliphatic amines and alcohols all increased the fraction of defect erythrocytes in a concentration-dependent manner. The results indicate that several fatty acids are very potent in their acute actions on erythrocytes, and that this effect is due to chain length rather than conformation.<br/><br>
<br/><br>
Conclusion: The technique offers a screening method for testing the harmful effects of small amounts of lipophilic substances on erythrocytes.}},
author = {{Söderberg, Lars and Haag, Linnea and Höglund, Peter and Stenberg, Pål and Wahlgren, Marie}},
issn = {{1879-0720}},
language = {{eng}},
number = {{4-5}},
pages = {{458--464}},
publisher = {{Elsevier}},
series = {{European Journal of Pharmaceutical Sciences}},
title = {{The effects of lipophilic substances on the shape of erythrocytes demonstrated by a new in vitro-method}},
url = {{http://dx.doi.org/10.1016/j.ejps.2008.11.011}},
doi = {{10.1016/j.ejps.2008.11.011}},
volume = {{36}},
year = {{2009}},
}