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Multiscale Characterization of Embryonic Long Bone Mineralization in Mice

Silva Barreto, Isabella LU ; Le Cann, Sophie LU ; Ahmed, Saima ; Sotiriou, Vivien ; Turunen, Mikael J. LU ; Johansson, Ulf LU ; Rodriguez-Fernandez, Angel LU ; Grünewald, Tilman A. ; Liebi, Marianne LU and Nowlan, Niamh C. , et al. (2020) In Advanced Science 7(21).
Abstract

Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone-like tissue with a hierarchical organization from the whole bone-scale down to sub-nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high-resolution synchrotron-based imaging techniques at several length scales. With micro- and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs... (More)

Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone-like tissue with a hierarchical organization from the whole bone-scale down to sub-nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high-resolution synchrotron-based imaging techniques at several length scales. With micro- and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
bone development, Fourier transform infra-red microspectroscopy, small- and wide-angle X-ray scattering, X-ray fluorescence spectroscopy, X-ray tomography
in
Advanced Science
volume
7
issue
21
article number
2002524
publisher
John Wiley and Sons
external identifiers
  • pmid:33173750
  • scopus:85091358771
ISSN
2198-3844
DOI
10.1002/advs.202002524
language
English
LU publication?
yes
id
a8c0267b-cdd5-4ee5-bbcd-dd5b150b22dc
date added to LUP
2020-11-20 14:32:50
date last changed
2021-06-08 01:27:10
@article{a8c0267b-cdd5-4ee5-bbcd-dd5b150b22dc,
  abstract     = {<p>Long bone mineralization occurs through endochondral ossification, where a cartilage template mineralizes into bone-like tissue with a hierarchical organization from the whole bone-scale down to sub-nano scale. Whereas this process has been extensively studied at the larger length scales, it remains unexplored at some of the smaller length scales. In this study, the changes in morphology, composition, and structure during embryonic mineralization of murine humeri are investigated using a range of high-resolution synchrotron-based imaging techniques at several length scales. With micro- and nanometer spatial resolution, the deposition of elements and the shaping of mineral platelets are followed. Rapid mineralization of the humeri occurs over approximately four days, where mineral to matrix ratio and calcium content in the most mineralized zone reach adult values shortly before birth. Interestingly, zinc is consistently found to be localized at the sites of ongoing new mineralization. The mineral platelets in the most recently mineralized regions are thicker, longer, narrower, and less aligned compared to those further into the mineralized region. In summary, this study demonstrates a specific spatial distribution of zinc, with highest concentration where new mineral is being deposited and that the newly formed mineral platelets undergo slight reshaping and reorganization during embryonic development.</p>},
  author       = {Silva Barreto, Isabella and Le Cann, Sophie and Ahmed, Saima and Sotiriou, Vivien and Turunen, Mikael J. and Johansson, Ulf and Rodriguez-Fernandez, Angel and Grünewald, Tilman A. and Liebi, Marianne and Nowlan, Niamh C. and Isaksson, Hanna},
  issn         = {2198-3844},
  language     = {eng},
  month        = {11},
  number       = {21},
  publisher    = {John Wiley and Sons},
  series       = {Advanced Science},
  title        = {Multiscale Characterization of Embryonic Long Bone Mineralization in Mice},
  url          = {http://dx.doi.org/10.1002/advs.202002524},
  doi          = {10.1002/advs.202002524},
  volume       = {7},
  year         = {2020},
}