Klk4t2 is a hormonally regulated transcript from the klk4 locus
(2021) In International Journal of Molecular Sciences 22(23).- Abstract
The human kallikrein-related peptidase 4 (KLK4) and the transcribed pseudogene KLKP1 are reported to be highly expressed in the prostate. When trying to clone transcripts of KLKP1, we partly failed. Instead, we identified an androgen-regulated transcript, KLK4T2, which appeared to be a splice variant of KLK4 that also contained exons of KLKP1. Expression analysis of KLK4, KLK4T2, and KLKP1 transcripts in prostate cancer cell lines showed high levels of KLKP1 transcripts in the nucleus and in unfractionated cell extract, whereas it was almost completely absent in the cytoplasmatic fraction. This was in contrast to KLK4 and KLK4T2, which displayed high to mod-erate levels in the cytoplasm. In patient cohorts we found significantly higher... (More)
The human kallikrein-related peptidase 4 (KLK4) and the transcribed pseudogene KLKP1 are reported to be highly expressed in the prostate. When trying to clone transcripts of KLKP1, we partly failed. Instead, we identified an androgen-regulated transcript, KLK4T2, which appeared to be a splice variant of KLK4 that also contained exons of KLKP1. Expression analysis of KLK4, KLK4T2, and KLKP1 transcripts in prostate cancer cell lines showed high levels of KLKP1 transcripts in the nucleus and in unfractionated cell extract, whereas it was almost completely absent in the cytoplasmatic fraction. This was in contrast to KLK4 and KLK4T2, which displayed high to mod-erate levels in the cytoplasm. In patient cohorts we found significantly higher expression of both KLK4T2 and KLK4 in benign prostatic hyperplasia compared to both primary prostate cancer and bone metastasis. Analysis of tissue panels demonstrated the highest expression of KLK4T2 in the prostate, but in contrast to the classical KLK4, relatively high levels were also found in placenta. So far, the function of KLK4T2 is still to be explored, but the structure of the translation product indicated that it generates a 17.4 kDa intracellular protein with possible regulatory function.
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- author
- Lundwall, Åke LU ; Ylitalo, Erik Bovinder ; Wikström, Pernilla and Brattsand, Maria
- organization
- publishing date
- 2021-12
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Bone metastasis, Kallikrein-related peptidases, KLK4, KLK4T2, KLKP1, Prostate cancer, Splice variant, Transcripts
- in
- International Journal of Molecular Sciences
- volume
- 22
- issue
- 23
- article number
- 13023
- publisher
- MDPI AG
- external identifiers
-
- scopus:85120163078
- pmid:34884832
- ISSN
- 1661-6596
- DOI
- 10.3390/ijms222313023
- language
- English
- LU publication?
- yes
- id
- a8d664cd-702c-4746-af2e-2fd7af64fcbf
- date added to LUP
- 2021-12-15 11:17:35
- date last changed
- 2024-06-15 22:44:59
@article{a8d664cd-702c-4746-af2e-2fd7af64fcbf,
abstract = {{<p>The human kallikrein-related peptidase 4 (KLK4) and the transcribed pseudogene KLKP1 are reported to be highly expressed in the prostate. When trying to clone transcripts of KLKP1, we partly failed. Instead, we identified an androgen-regulated transcript, KLK4T2, which appeared to be a splice variant of KLK4 that also contained exons of KLKP1. Expression analysis of KLK4, KLK4T2, and KLKP1 transcripts in prostate cancer cell lines showed high levels of KLKP1 transcripts in the nucleus and in unfractionated cell extract, whereas it was almost completely absent in the cytoplasmatic fraction. This was in contrast to KLK4 and KLK4T2, which displayed high to mod-erate levels in the cytoplasm. In patient cohorts we found significantly higher expression of both KLK4T2 and KLK4 in benign prostatic hyperplasia compared to both primary prostate cancer and bone metastasis. Analysis of tissue panels demonstrated the highest expression of KLK4T2 in the prostate, but in contrast to the classical KLK4, relatively high levels were also found in placenta. So far, the function of KLK4T2 is still to be explored, but the structure of the translation product indicated that it generates a 17.4 kDa intracellular protein with possible regulatory function.</p>}},
author = {{Lundwall, Åke and Ylitalo, Erik Bovinder and Wikström, Pernilla and Brattsand, Maria}},
issn = {{1661-6596}},
keywords = {{Bone metastasis; Kallikrein-related peptidases; KLK4; KLK4T2; KLKP1; Prostate cancer; Splice variant; Transcripts}},
language = {{eng}},
number = {{23}},
publisher = {{MDPI AG}},
series = {{International Journal of Molecular Sciences}},
title = {{Klk4t2 is a hormonally regulated transcript from the klk4 locus}},
url = {{http://dx.doi.org/10.3390/ijms222313023}},
doi = {{10.3390/ijms222313023}},
volume = {{22}},
year = {{2021}},
}