COMP acts as a catalyst in collagen fibrillogenesis
(2007) In Journal of Biological Chemistry 282(43). p.31166-31173- Abstract
- We have previously reported that COMP (cartilage oligomeric matrix protein) is prominent in cartilage but is also present in tendon and binds to collagens I and II with high affinity. Here we show that COMP influences the fibril formation of these collagens. Fibril formation in the presence of pentameric COMP was much faster, and the amount of collagen in fibrillar form was markedly increased. Monomeric COMP, lacking the N-terminal coiled-coil linker domain, decelerated fibrillogenesis. The data show that stimulation of collagen fibrillogenesis depends on the pentameric nature of COMP and not only on collagen binding. COMP interacts primarily with free collagen I and II molecules, bringing several molecules to close proximity, apparently... (More)
- We have previously reported that COMP (cartilage oligomeric matrix protein) is prominent in cartilage but is also present in tendon and binds to collagens I and II with high affinity. Here we show that COMP influences the fibril formation of these collagens. Fibril formation in the presence of pentameric COMP was much faster, and the amount of collagen in fibrillar form was markedly increased. Monomeric COMP, lacking the N-terminal coiled-coil linker domain, decelerated fibrillogenesis. The data show that stimulation of collagen fibrillogenesis depends on the pentameric nature of COMP and not only on collagen binding. COMP interacts primarily with free collagen I and II molecules, bringing several molecules to close proximity, apparently promoting further assembly. These assemblies further join in discrete steps to a narrow distribution of completed fibril diameters of 149 +/- 16 nm with a banding pattern of 67 nm. COMP is not found associated with the mature fibril and dissociates from the collagen molecules or their early assemblies. However, a few COMP molecules are found bound to more loosely associated molecules at the tip/end of the growing fibril. Thus, COMP appears to catalyze the fibril formation by promoting early association of collagen molecules leading to increased rate of fibrillogenesis and more distinct organization of the fibrils. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1141748
- author
- Halasz, Krisztina LU ; Kassner, Anja LU ; Mörgelin, Matthias LU and Heinegård, Dick LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 282
- issue
- 43
- pages
- 31166 - 31173
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:17716974
- wos:000250309200006
- scopus:35748950738
- pmid:17716974
- ISSN
- 1083-351X
- DOI
- 10.1074/jbc.M705735200
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Division III (013230700), Connective Tissue Biology (013230151), Department of Experimental Medical Science (013210000), Division of Infection Medicine (BMC) (013024020)
- id
- a93e384b-b5ed-4d17-ae5f-cdbdb653f9d3 (old id 1141748)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17716974&dopt=Abstract
- date added to LUP
- 2016-04-04 07:49:21
- date last changed
- 2022-03-15 07:25:17
@article{a93e384b-b5ed-4d17-ae5f-cdbdb653f9d3, abstract = {{We have previously reported that COMP (cartilage oligomeric matrix protein) is prominent in cartilage but is also present in tendon and binds to collagens I and II with high affinity. Here we show that COMP influences the fibril formation of these collagens. Fibril formation in the presence of pentameric COMP was much faster, and the amount of collagen in fibrillar form was markedly increased. Monomeric COMP, lacking the N-terminal coiled-coil linker domain, decelerated fibrillogenesis. The data show that stimulation of collagen fibrillogenesis depends on the pentameric nature of COMP and not only on collagen binding. COMP interacts primarily with free collagen I and II molecules, bringing several molecules to close proximity, apparently promoting further assembly. These assemblies further join in discrete steps to a narrow distribution of completed fibril diameters of 149 +/- 16 nm with a banding pattern of 67 nm. COMP is not found associated with the mature fibril and dissociates from the collagen molecules or their early assemblies. However, a few COMP molecules are found bound to more loosely associated molecules at the tip/end of the growing fibril. Thus, COMP appears to catalyze the fibril formation by promoting early association of collagen molecules leading to increased rate of fibrillogenesis and more distinct organization of the fibrils.}}, author = {{Halasz, Krisztina and Kassner, Anja and Mörgelin, Matthias and Heinegård, Dick}}, issn = {{1083-351X}}, language = {{eng}}, number = {{43}}, pages = {{31166--31173}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{COMP acts as a catalyst in collagen fibrillogenesis}}, url = {{http://dx.doi.org/10.1074/jbc.M705735200}}, doi = {{10.1074/jbc.M705735200}}, volume = {{282}}, year = {{2007}}, }