Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Molecularly imprinted microspheres as antibody binding mimics

Ye, Lei LU orcid and Mosbach, Klaus LU (2001) 9th International Conference on Polymer-Based Technology In Reactive and Functional Polymers 48(1-3). p.149-157
Abstract

Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of... (More)

Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.

(Less)
Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme-linked immunosorbent assay, Immunoassay, Microspheres, Molecular imprinting, Precipitation polymerization
in
Reactive and Functional Polymers
volume
48
issue
1-3
pages
9 pages
publisher
Elsevier
conference name
9th International Conference on Polymer-Based Technology
conference location
China
conference dates
2000-05-21 - 2000-05-21
external identifiers
  • scopus:0035795570
ISSN
1381-5148
DOI
10.1016/S1381-5148(01)00050-5
language
English
LU publication?
yes
id
aa07a0a4-ac38-4d3d-9391-227f44e6959b
date added to LUP
2019-06-18 09:00:00
date last changed
2025-10-14 12:21:56
@article{aa07a0a4-ac38-4d3d-9391-227f44e6959b,
  abstract     = {{<p>Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.</p>}},
  author       = {{Ye, Lei and Mosbach, Klaus}},
  issn         = {{1381-5148}},
  keywords     = {{Enzyme-linked immunosorbent assay; Immunoassay; Microspheres; Molecular imprinting; Precipitation polymerization}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{1-3}},
  pages        = {{149--157}},
  publisher    = {{Elsevier}},
  series       = {{Reactive and Functional Polymers}},
  title        = {{Molecularly imprinted microspheres as antibody binding mimics}},
  url          = {{http://dx.doi.org/10.1016/S1381-5148(01)00050-5}},
  doi          = {{10.1016/S1381-5148(01)00050-5}},
  volume       = {{48}},
  year         = {{2001}},
}