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Molecularly imprinted microspheres as antibody binding mimics

Ye, Lei LU and Mosbach, Klaus LU (2001) 9th International Conference on Polymer-Based Technology In Reactive and Functional Polymers 48(1-3). p.149-157
Abstract

Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of... (More)

Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.

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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Enzyme-linked immunosorbent assay, Immunoassay, Microspheres, Molecular imprinting, Precipitation polymerization
in
Reactive and Functional Polymers
volume
48
issue
1-3
pages
9 pages
publisher
Elsevier
conference name
9th International Conference on Polymer-Based Technology
conference location
China
conference dates
2000-05-21 - 2000-05-21
external identifiers
  • scopus:0035795570
ISSN
1381-5148
DOI
10.1016/S1381-5148(01)00050-5
language
English
LU publication?
yes
id
aa07a0a4-ac38-4d3d-9391-227f44e6959b
date added to LUP
2019-06-18 09:00:00
date last changed
2020-12-15 02:53:39
@article{aa07a0a4-ac38-4d3d-9391-227f44e6959b,
  abstract     = {<p>Molecular imprinting is an emerging technique for preparing artificial antibodies that have potential applications in affinity-based separations, biomimetic sensors and assays. The conventional imprinting methods either deliver a monolith that is mechanically ground to irregular particles - a time-consuming process with low yield, or involves complicated polymerization process by which spherical beads may be obtained, but with frequently compromised binding specificity. We have recently developed a novel molecular imprinting method that can deliver microspheres bearing imprinted binding sites, quickly, cleanly and in good yield. Imprinted uniform microspheres are synthesized by precipitation polymerization of a dilute solution of functional monomer and crosslinker in the presence of a print molecule. The method is generally applicable to a large variety of target molecules. For the first time, an enzyme probe is successfully used in a competitive herbicide assay using molecularly imprinted microspheres as the affinity adsorbents.</p>},
  author       = {Ye, Lei and Mosbach, Klaus},
  issn         = {1381-5148},
  language     = {eng},
  month        = {05},
  number       = {1-3},
  pages        = {149--157},
  publisher    = {Elsevier},
  series       = {Reactive and Functional Polymers},
  title        = {Molecularly imprinted microspheres as antibody binding mimics},
  url          = {http://dx.doi.org/10.1016/S1381-5148(01)00050-5},
  doi          = {10.1016/S1381-5148(01)00050-5},
  volume       = {48},
  year         = {2001},
}