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Elevated circulating effector memory T cells but similar levels of regulatory T cells in patients with type 2 diabetes mellitus and cardiovascular disease

Rattik, Sara LU ; Engelbertsen, Daniel LU ; Wigren, Maria LU ; Ljungcrantz, Irena LU ; Östling, Gerd LU ; Persson, Margaretha LU orcid ; Nordin Fredrikson, Gunilla LU ; Bengtsson, Eva LU orcid ; Nilsson, Jan LU and Björkbacka, Harry LU orcid (2019) In Diabetes and Vascular Disease Research 16(3). p.270-280
Abstract

Type 2 diabetes mellitus is associated with an elevated risk of cardiovascular disease, but the mechanism through which diabetes contributes to cardiovascular disease development remains incompletely understood. In this study, we compared the association of circulating regulatory T cells, naïve T cells, effector memory T cells or central memory T cells with cardiovascular disease in patients with and without type 2 diabetes mellitus. Percentage of circulating T cell subsets was analysed by flow cytometry in type 2 diabetes mellitus subjects with and without prevalent cardiovascular disease as well as in non-diabetic subjects with and without prevalent cardiovascular disease from the Malmö SUMMIT cohort. Subjects with type 2 diabetes... (More)

Type 2 diabetes mellitus is associated with an elevated risk of cardiovascular disease, but the mechanism through which diabetes contributes to cardiovascular disease development remains incompletely understood. In this study, we compared the association of circulating regulatory T cells, naïve T cells, effector memory T cells or central memory T cells with cardiovascular disease in patients with and without type 2 diabetes mellitus. Percentage of circulating T cell subsets was analysed by flow cytometry in type 2 diabetes mellitus subjects with and without prevalent cardiovascular disease as well as in non-diabetic subjects with and without prevalent cardiovascular disease from the Malmö SUMMIT cohort. Subjects with type 2 diabetes mellitus had elevated percentages of effector memory T cells (CD4+CD45RO+CD62L–; 21.8% ± 11.2% vs 17.0% ± 9.2% in non-type 2 diabetes mellitus, p < 0.01) and central memory T cells (CD4+CD45RO+CD62L+; 38.0% ± 10.7% vs 36.0% ± 9.5% in non-type 2 diabetes mellitus, p < 0.01). In contrast, the frequency of naïve T cells was reduced (CD4+CD45RO–CD62L+, 35.0% ± 16.5% vs 42.9% ± 14.4% in non-type 2 diabetes mellitus, p < 0.001). The proportion of effector memory T cells was increased in type 2 diabetes mellitus subjects with cardiovascular disease as compared to those without (26.4% ± 11.5% vs 18.4% ± 10.2%, p < 0.05), while no difference in regulatory T cells was observed between these two patient groups. This study identifies effector memory T cells as a potential cellular biomarker for cardiovascular disease among subjects with type 2 diabetes mellitus, suggesting a state of exacerbated immune activation in type 2 diabetes mellitus patients with cardiovascular disease.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
adaptive immunity, cardiovascular disease, memory T cells, regulatory T cells, Type 2 diabetes
in
Diabetes and Vascular Disease Research
volume
16
issue
3
pages
270 - 280
publisher
SAGE Publications
external identifiers
  • scopus:85059016898
  • pmid:30574794
ISSN
1479-1641
DOI
10.1177/1479164118817942
language
English
LU publication?
yes
id
aae7cd27-264f-4a59-980a-a8aee57cd754
date added to LUP
2019-01-07 14:06:09
date last changed
2024-03-18 21:02:32
@article{aae7cd27-264f-4a59-980a-a8aee57cd754,
  abstract     = {{<p>Type 2 diabetes mellitus is associated with an elevated risk of cardiovascular disease, but the mechanism through which diabetes contributes to cardiovascular disease development remains incompletely understood. In this study, we compared the association of circulating regulatory T cells, naïve T cells, effector memory T cells or central memory T cells with cardiovascular disease in patients with and without type 2 diabetes mellitus. Percentage of circulating T cell subsets was analysed by flow cytometry in type 2 diabetes mellitus subjects with and without prevalent cardiovascular disease as well as in non-diabetic subjects with and without prevalent cardiovascular disease from the Malmö SUMMIT cohort. Subjects with type 2 diabetes mellitus had elevated percentages of effector memory T cells (CD4<sup>+</sup>CD45RO<sup>+</sup>CD62L<sup>–</sup>; 21.8% ± 11.2% vs 17.0% ± 9.2% in non-type 2 diabetes mellitus, p &lt; 0.01) and central memory T cells (CD4<sup>+</sup>CD45RO<sup>+</sup>CD62L<sup>+</sup>; 38.0% ± 10.7% vs 36.0% ± 9.5% in non-type 2 diabetes mellitus, p &lt; 0.01). In contrast, the frequency of naïve T cells was reduced (CD4<sup>+</sup>CD45RO<sup>–</sup>CD62L<sup>+</sup>, 35.0% ± 16.5% vs 42.9% ± 14.4% in non-type 2 diabetes mellitus, p &lt; 0.001). The proportion of effector memory T cells was increased in type 2 diabetes mellitus subjects with cardiovascular disease as compared to those without (26.4% ± 11.5% vs 18.4% ± 10.2%, p &lt; 0.05), while no difference in regulatory T cells was observed between these two patient groups. This study identifies effector memory T cells as a potential cellular biomarker for cardiovascular disease among subjects with type 2 diabetes mellitus, suggesting a state of exacerbated immune activation in type 2 diabetes mellitus patients with cardiovascular disease.</p>}},
  author       = {{Rattik, Sara and Engelbertsen, Daniel and Wigren, Maria and Ljungcrantz, Irena and Östling, Gerd and Persson, Margaretha and Nordin Fredrikson, Gunilla and Bengtsson, Eva and Nilsson, Jan and Björkbacka, Harry}},
  issn         = {{1479-1641}},
  keywords     = {{adaptive immunity; cardiovascular disease; memory T cells; regulatory T cells; Type 2 diabetes}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{270--280}},
  publisher    = {{SAGE Publications}},
  series       = {{Diabetes and Vascular Disease Research}},
  title        = {{Elevated circulating effector memory T cells but similar levels of regulatory T cells in patients with type 2 diabetes mellitus and cardiovascular disease}},
  url          = {{http://dx.doi.org/10.1177/1479164118817942}},
  doi          = {{10.1177/1479164118817942}},
  volume       = {{16}},
  year         = {{2019}},
}