Dependency of Glucose Homeostasis on Pancreatic Enzymes with Special Reference to Amylase; Study on Healthy and Exocrine Pancreatic Insufficient Pigs
Wychowański, Piotr ; Pierzynowski, Stefan G. LU ; Zaworski, Kamil LU ; Gallotto, Robert ; Szkopek, Dominika ; Woliński, Jarosław LU ; Donaldson, Janine ; Jacek, Tomasz and Pierzynowska, Kateryna LU
(2026)
In Biomolecules
16(1).
- Abstract
We aimed to highlight the roles of the pancreatic enzymes, with special reference to amylase, on glucose homeostasis in healthy pigs and in pigs with exocrine pancreatic insufficiency (EPI). Healthy pigs fed a high-fat diet (HFD) were subjected to mixed meal tolerance tests (MMTTs) and pancreatic enzyme treatments, and then blood glucose and insulin concentrations were determined. Following the development of surgically induced EPI, the same experiment was then repeated on the pigs. A significantly lower net postprandial glycemic response was observed in pigs with EPI compared to healthy pigs. Net postprandial glycemic response was not affected by enzyme supplementation during the MMTTs in healthy pigs, but it was affected by adaptation... (More)
We aimed to highlight the roles of the pancreatic enzymes, with special reference to amylase, on glucose homeostasis in healthy pigs and in pigs with exocrine pancreatic insufficiency (EPI). Healthy pigs fed a high-fat diet (HFD) were subjected to mixed meal tolerance tests (MMTTs) and pancreatic enzyme treatments, and then blood glucose and insulin concentrations were determined. Following the development of surgically induced EPI, the same experiment was then repeated on the pigs. A significantly lower net postprandial glycemic response was observed in pigs with EPI compared to healthy pigs. Net postprandial glycemic response was not affected by enzyme supplementation during the MMTTs in healthy pigs, but it was affected by adaptation to macronutrient components of the MMTT test meal, both in healthy and EPI pigs. Net postprandial glycemic response and insulin release curves reached higher levels in Creon-treated EPI pigs compared to amylase-treated EPI pigs. In summary, glucose homeostasis mechanisms in EPI pigs were downregulated compared to healthy animals. Creon supplementation during EPI significantly increased postprandial glucose level, while amylase treatment had the opposite effect, which could be explained by its metabolic actions.
(Less)
- author
- Wychowański, Piotr
; Pierzynowski, Stefan G.
LU
; Zaworski, Kamil
LU
; Gallotto, Robert
; Szkopek, Dominika
; Woliński, Jarosław
LU
; Donaldson, Janine
; Jacek, Tomasz
and Pierzynowska, Kateryna
LU
- organization
- publishing date
- 2026-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- amylase, glucose metabolism, insulin release, mixed meal tolerance test, MMTT, pancreatic enzyme replacement therapy, PERT
- in
- Biomolecules
- volume
- 16
- issue
- 1
- article number
- 172
- publisher
- MDPI AG
- external identifiers
-
- pmid:41594712
- scopus:105028840685
- ISSN
- 2218-273X
- DOI
- 10.3390/biom16010172
- language
- English
- LU publication?
- yes
- id
- ab924553-626e-4c2a-bd0b-d6293267abfa
- date added to LUP
- 2026-02-25 10:54:30
- date last changed
- 2026-02-25 10:55:29
@article{ab924553-626e-4c2a-bd0b-d6293267abfa,
abstract = {{<p>We aimed to highlight the roles of the pancreatic enzymes, with special reference to amylase, on glucose homeostasis in healthy pigs and in pigs with exocrine pancreatic insufficiency (EPI). Healthy pigs fed a high-fat diet (HFD) were subjected to mixed meal tolerance tests (MMTTs) and pancreatic enzyme treatments, and then blood glucose and insulin concentrations were determined. Following the development of surgically induced EPI, the same experiment was then repeated on the pigs. A significantly lower net postprandial glycemic response was observed in pigs with EPI compared to healthy pigs. Net postprandial glycemic response was not affected by enzyme supplementation during the MMTTs in healthy pigs, but it was affected by adaptation to macronutrient components of the MMTT test meal, both in healthy and EPI pigs. Net postprandial glycemic response and insulin release curves reached higher levels in Creon-treated EPI pigs compared to amylase-treated EPI pigs. In summary, glucose homeostasis mechanisms in EPI pigs were downregulated compared to healthy animals. Creon supplementation during EPI significantly increased postprandial glucose level, while amylase treatment had the opposite effect, which could be explained by its metabolic actions.</p>}},
author = {{Wychowański, Piotr and Pierzynowski, Stefan G. and Zaworski, Kamil and Gallotto, Robert and Szkopek, Dominika and Woliński, Jarosław and Donaldson, Janine and Jacek, Tomasz and Pierzynowska, Kateryna}},
issn = {{2218-273X}},
keywords = {{amylase; glucose metabolism; insulin release; mixed meal tolerance test; MMTT; pancreatic enzyme replacement therapy; PERT}},
language = {{eng}},
number = {{1}},
publisher = {{MDPI AG}},
series = {{Biomolecules}},
title = {{Dependency of Glucose Homeostasis on Pancreatic Enzymes with Special Reference to Amylase; Study on Healthy and Exocrine Pancreatic Insufficient Pigs}},
url = {{http://dx.doi.org/10.3390/biom16010172}},
doi = {{10.3390/biom16010172}},
volume = {{16}},
year = {{2026}},
}