A two-colour immunofluorescence test with a monoclonal human proinsulin antibody improves the assay for islet cell antibodies
Madsen, O D ; Landin Olsson, M LU ; Bille, G ; Sundkvist, G LU ; Lernmark, A LU
; Dahlqvist, G
and Ludvigsson, J
(1986)
In Diabetologia
29(2).
p.8-115
- Abstract
The conventional indirect immunofluorescence assay for islet cell antibodies was compared with a two-colour immunofluorescent assay to detect both islet cell antibodies with fluorescein isothiocyanate-labeled rabbit anti-human IgG and pancreatic B cells with a monoclonal human proinsulin antibody and Texas red-labeled sheep anti-mouse IgG. Determinations of end-point titres showed a correlation between the new two-colour immunofluorescent assay and the conventional indirect immunofluorescent assay in 1) selected sera positive for islet cell antibodies and insulin autoantibodies rs = 0.93 (p less than 0.01) or for islet cell antibodies alone rs = 0.99 (p less than 0.005) and 2) sera from children or young adults with newly diagnosed Type... (More)
The conventional indirect immunofluorescence assay for islet cell antibodies was compared with a two-colour immunofluorescent assay to detect both islet cell antibodies with fluorescein isothiocyanate-labeled rabbit anti-human IgG and pancreatic B cells with a monoclonal human proinsulin antibody and Texas red-labeled sheep anti-mouse IgG. Determinations of end-point titres showed a correlation between the new two-colour immunofluorescent assay and the conventional indirect immunofluorescent assay in 1) selected sera positive for islet cell antibodies and insulin autoantibodies rs = 0.93 (p less than 0.01) or for islet cell antibodies alone rs = 0.99 (p less than 0.005) and 2) sera from children or young adults with newly diagnosed Type 1 (insulin-dependent) diabetes rs = 0.95 (p less than 0.0001). No interference between the monoclonal human proinsulin antibodies and islet cell antibodies with or without insulin autoantibodies or between the two second fluorescent antibodies was detected. It is concluded that the two-colour immunofluorescence assay is advantageous since it is possible to mix the reagents to avoid a more time-consuming and technically complicated assay, the presence of B cells can be confirmed in each section to permit detection of B cell cytoplasmic antibodies and microscopic evaluation is easier and more accurate, particularly in islet cell antibody negative samples.
(Less)
- author
- Madsen, O D
; Landin Olsson, M
LU
; Bille, G
; Sundkvist, G
LU
; Lernmark, A
LU
; Dahlqvist, G
and Ludvigsson, J
- organization
- publishing date
- 1986
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Aged, Antibodies, Monoclonal, Autoantibodies/analysis, Female, Fluorescent Antibody Technique, Humans, Islets of Langerhans/immunology, Proinsulin/analysis
- in
- Diabetologia
- volume
- 29
- issue
- 2
- pages
- 8 - 115
- publisher
- Springer
- external identifiers
-
- scopus:0022591168
- pmid:3516766
- ISSN
- 0012-186X
- DOI
- 10.1007/BF00456121
- language
- English
- LU publication?
- yes
- id
- ab9b2750-64ff-40ec-993f-1c83ca04d392
- date added to LUP
- 2021-09-15 16:20:32
- date last changed
- 2024-03-13 08:13:45
@article{ab9b2750-64ff-40ec-993f-1c83ca04d392,
abstract = {{<p>The conventional indirect immunofluorescence assay for islet cell antibodies was compared with a two-colour immunofluorescent assay to detect both islet cell antibodies with fluorescein isothiocyanate-labeled rabbit anti-human IgG and pancreatic B cells with a monoclonal human proinsulin antibody and Texas red-labeled sheep anti-mouse IgG. Determinations of end-point titres showed a correlation between the new two-colour immunofluorescent assay and the conventional indirect immunofluorescent assay in 1) selected sera positive for islet cell antibodies and insulin autoantibodies rs = 0.93 (p less than 0.01) or for islet cell antibodies alone rs = 0.99 (p less than 0.005) and 2) sera from children or young adults with newly diagnosed Type 1 (insulin-dependent) diabetes rs = 0.95 (p less than 0.0001). No interference between the monoclonal human proinsulin antibodies and islet cell antibodies with or without insulin autoantibodies or between the two second fluorescent antibodies was detected. It is concluded that the two-colour immunofluorescence assay is advantageous since it is possible to mix the reagents to avoid a more time-consuming and technically complicated assay, the presence of B cells can be confirmed in each section to permit detection of B cell cytoplasmic antibodies and microscopic evaluation is easier and more accurate, particularly in islet cell antibody negative samples.</p>}},
author = {{Madsen, O D and Landin Olsson, M and Bille, G and Sundkvist, G and Lernmark, A and Dahlqvist, G and Ludvigsson, J}},
issn = {{0012-186X}},
keywords = {{Aged; Antibodies, Monoclonal; Autoantibodies/analysis; Female; Fluorescent Antibody Technique; Humans; Islets of Langerhans/immunology; Proinsulin/analysis}},
language = {{eng}},
number = {{2}},
pages = {{8--115}},
publisher = {{Springer}},
series = {{Diabetologia}},
title = {{A two-colour immunofluorescence test with a monoclonal human proinsulin antibody improves the assay for islet cell antibodies}},
url = {{http://dx.doi.org/10.1007/BF00456121}},
doi = {{10.1007/BF00456121}},
volume = {{29}},
year = {{1986}},
}