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Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase

Fu, Tian-Min; Li, Dan; Nan, Jie LU ; Li, Lanfen; Xue, Yafeng and Su, Xiao-Dong LU (2007) In Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00 63(Pt 12). p.8-1026
Abstract

As a substrate of extracellular signal-related kinase (ERK), the p90 ribosome S6 kinase 1 (RSK1) is at the terminus of the Ras/ERK pathway. Residues 411-735 of human RSK1, covering the C-terminal serine/threonine kinase catalytic domain and the functionally important tail, were cloned into an Escherichia coli expression vector. The protein was expressed, purified and crystallized. The crystals diffracted to 2.7 A and belonged to space group P2(1), with unit-cell parameters a = 39.8, b = 143.8, c = 59.9 A, beta = 95.7 degrees.

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author
organization
publishing date
type
Contribution to journal
publication status
published
keywords
Crystallization, Crystallography, X-Ray, Humans, Protein-Serine-Threonine Kinases, Ribosomal Protein S6 Kinases, 90-kDa
in
Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00
volume
63
issue
Pt 12
pages
3 pages
publisher
Wiley-Blackwell
external identifiers
  • scopus:36849085969
ISSN
2053-230X
DOI
10.1107/S1744309107051329
language
English
LU publication?
yes
id
ac1dbddd-5583-4042-a39e-8c9e1673f595
date added to LUP
2016-09-07 22:53:56
date last changed
2017-01-01 08:33:32
@article{ac1dbddd-5583-4042-a39e-8c9e1673f595,
  abstract     = {<p>As a substrate of extracellular signal-related kinase (ERK), the p90 ribosome S6 kinase 1 (RSK1) is at the terminus of the Ras/ERK pathway. Residues 411-735 of human RSK1, covering the C-terminal serine/threonine kinase catalytic domain and the functionally important tail, were cloned into an Escherichia coli expression vector. The protein was expressed, purified and crystallized. The crystals diffracted to 2.7 A and belonged to space group P2(1), with unit-cell parameters a = 39.8, b = 143.8, c = 59.9 A, beta = 95.7 degrees.</p>},
  author       = {Fu, Tian-Min and Li, Dan and Nan, Jie and Li, Lanfen and Xue, Yafeng and Su, Xiao-Dong},
  issn         = {2053-230X},
  keyword      = {Crystallization,Crystallography, X-Ray,Humans,Protein-Serine-Threonine Kinases,Ribosomal Protein S6 Kinases, 90-kDa},
  language     = {eng},
  month        = {12},
  number       = {Pt 12},
  pages        = {8--1026},
  publisher    = {Wiley-Blackwell},
  series       = {Acta Crystallographica. Section F: Structural Biology and Crystallization Communications2005-01-01+01:002014-01-01+01:00},
  title        = {Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase},
  url          = {http://dx.doi.org/10.1107/S1744309107051329},
  volume       = {63},
  year         = {2007},
}