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A Biomimetic, Copolymeric Membrane for Cell-Stretch Experiments with Pulmonary Epithelial Cells at the Air-Liquid Interface

Doryab, Ali ; Taskin, Mehmet Berat ; Stahlhut, Philipp ; Schröppel, Andreas ; Wagner, Darcy E. LU ; Groll, Jürgen and Schmid, Otmar (2021) In Advanced Functional Materials 31(10).
Abstract

Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 μm) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air–liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for... (More)

Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 μm) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air–liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
alveolar-capillary barrier, cyclic mechanical stretch, hybrid polymers, in vitro cell-stretch model, tunable ultra-thin biphasic membrane
in
Advanced Functional Materials
volume
31
issue
10
article number
2004707
publisher
Wiley-VCH Verlag
external identifiers
  • scopus:85096761686
ISSN
1616-301X
DOI
10.1002/adfm.202004707
language
English
LU publication?
yes
id
ac64c02d-eb3c-45a8-abd5-e5184227d965
date added to LUP
2020-12-28 21:16:17
date last changed
2021-04-17 22:58:42
@article{ac64c02d-eb3c-45a8-abd5-e5184227d965,
  abstract     = {<p>Chronic respiratory diseases are among the leading causes of death worldwide, but only symptomatic therapies are available for terminal illness. This in part reflects a lack of biomimetic in vitro models that can imitate the complex environment and physiology of the lung. Here, a copolymeric membrane consisting of poly(ε-)caprolactone and gelatin with tunable properties, resembling the main characteristics of the alveolar basement membrane is introduced. The thin bioinspired membrane (0.5 μm) is stretchable (up to 25% linear strain) with appropriate surface wettability and porosity for culturing lung epithelial cells under air–liquid interface conditions. The unique biphasic concept of this membrane provides optimum characteristics for initial cell growth (phase I) and then switch to biomimetic properties for cyclic cell-stretch experiments (phase II). It is showed that physiologic cyclic mechanical stretch improves formation of F-actin cytoskeleton filaments and tight junctions while non-physiologic over-stretch induces cell apoptosis, activates inflammatory response (IL-8), and impairs epithelial barrier integrity. It is also demonstrated that cyclic physiologic stretch can enhance the cellular uptake of nanoparticles. Since this membrane offers considerable advantages over currently used membranes, it may lead the way to more biomimetic in vitro models of the lung for translation of in vitro response studies into clinical outcome.</p>},
  author       = {Doryab, Ali and Taskin, Mehmet Berat and Stahlhut, Philipp and Schröppel, Andreas and Wagner, Darcy E. and Groll, Jürgen and Schmid, Otmar},
  issn         = {1616-301X},
  language     = {eng},
  number       = {10},
  publisher    = {Wiley-VCH Verlag},
  series       = {Advanced Functional Materials},
  title        = {A Biomimetic, Copolymeric Membrane for Cell-Stretch Experiments with Pulmonary Epithelial Cells at the Air-Liquid Interface},
  url          = {http://dx.doi.org/10.1002/adfm.202004707},
  doi          = {10.1002/adfm.202004707},
  volume       = {31},
  year         = {2021},
}